Rho specific GTPase activating protein
Secretory Pathway-Dependent Localization of the Saccharomyces cerevisiae Rho GTPase-Activating Protein Rgd1p at Growth Sites
... to specific sites on the plasma mem- brane (11, 44, ...The GTPase Rho1p was found to be localized to the Golgi apparatus and post-Golgi vesicles, and its localization is disrupted in mutants acting at late ...
11
Purification and functional characterization of brain and recombinant chimaerin, a p21rac GTPase activating protein
... brain protein of 45 kDa was usually detected by western assay using anti-chimaerin ...the rho subfamily in rat tissues (Manser et al, ...brain specific pi 00 GAP acted on ra d and ...testis ...
243
Rho GTPase activating protein 18: a biomarker associated with good prognosis in invasive breast cancer
... single specific band at the predicted size (75 kDa) of ARHGAP18 protein, confirming the specificity of the antibody, Figure ...ARHGAP18 protein was localised in the nuclei and cytoplasm of tumour ...
29
Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p
... and Rho-type GTPases are likely to be components of a general mechanism used for growth guidance in many eukaryotic cell ...and Rho-type GTPases mediate extracellular signals that guide the migration of ...
14
The Rgd1p Rho GTPase-Activating Protein and the Mid2p Cell Wall Sensor Are Required at Low pH for Protein Kinase C Pathway Activation and Cell Survival in Saccharomyces cerevisiae
... interacts with RGD1. MID2 deletion exacerbates the rgd1 ⌬ strain mortality observed in stationary phase, whereas its over- expression suppresses the rgd1 ⌬ growth defect. In fact, the RGD1 gene, which encodes the ...
12
Biochemical characterisation of rho/rac GTPases and their GTPase activating proteins in humans and Dictyostelium discoideum
... the GTPase activity and interactions with target proteins have been ...intrinsic GTPase activity of the protein and renders the GTPase resistant to acceleration by GAP although the interaction ...
240
Ras GTPase-Activating Protein Regulation of Actin Cytoskeleton and Hyphal Polarity in Aspergillus nidulans
... Ras-like protein that does not appear to play a role in hyphal growth ...second protein (the AN3434 product [branch 4]) is more distantly related to RasA/B and, notably, appears to be specific of ...
13
Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast
... Not all cell fusion proteins are induced by pheromone; however, many localize to the tip of the mating projection (Trueheart et al. 1987; Gehrung and Snyder 1990; Elion et al. 1995; Valtz and Herskowitz 1996; Evange- ...
14
GTPase-Activating Proteins for Cdc42
... The Rho-type GTPase, Cdc42, has been implicated in a variety of functions in the yeast life cycle, including septin organization for cytokinesis, pheromone response, and haploid invasive ...called ...
12
Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia
... antibodies specific for IgG subclasses 1 to 4 (The Binding Site, Schwetzingen, Germany) were substituted for the FITC-labeled goat anti-human IgG antibody, and AF568-labeled donkey anti-sheep IgG (Invitrogen; ...
12
Lymphangioleiomyomatosis — a wolf in sheep’s clothing
... cells (56). Mice that are heterozygous for both TSC2 and Beclin-1 (an autophagy effector) show a decrease in the number of renal cystadenomas compared with mice heterozygous for Tsc2 alone, suggesting that inhibition of ...
11
The Role of the GTPase Rho in T cell Adhesion and Migration
... of Rho function changes the migration pattern of thymocytes such that the cells become more likely to encounter antigen in a ‘negatively’ selecting ...of Rho function has been shown to prevent T cells ...
210
Defining diverse mechanisms that regulate the activity of Dbl-family guanine nucleotide exchange factors
... the GTPase and is often sufficient for exchange in vitro ...regulation specific to each GEF and can participate in functions such as determining sub-cellular localization, auto-inhibiting the GEF by ...
158
Role of IQGAP3 in metastasis and epithelial–mesenchymal transition in human hepatocellular carcinoma
... and protein in HCC cell lines (Hep3B, SMMC-7721, HCCC-9810, HepG2, BEL-7404, HCCLM3, QGY-7701, Huh7, and MHCC97H) and normal liver epithelial cells LO 2 , as examined by western blotting and quantitative ...
14
Regulation and structure/function relationships of the Rac1 GTPase-activating protein, n-chimaerin
... bacterial protein expression system has been the use of fusion ...desired protein whereby its cDNA is cloned either 3' to the sequence o f a ...fusion protein is hence dependent on the ...
278
Regulation of RhoA GTPase signaling by guanine nucleotide exchange factors in response to extracellular matrix adhesion and DNA damage
... Figure 2.3: Knockdown of Lsc/p115 RhoGEF and LARG decreases stress fiber and focal adhesion formation and RhoA activity downstream of FN. (A) REF52 cells were transfected with either control or Lsc+LARG siRNA ...
160
C elegans Rab GTPase activating protein TBC 2 promotes cell corpse degradation by regulating the small GTPase RAB 5
... including protein-protein interactions, phospholipid interactions, phosphorylation and proteolytic degradation (Bernards and Settleman, ...domain-containing protein, has an absolute requirement for ...
11
Isolation and characterisation of α2-chimaerin cDNA encoding a p21rac GTPase-activating protein with an SH2 domain
... of novel sequence that has no counterpart in the database. 131 did not have a long open reading frame in either strands, bearing in mind that the sequence is not base- perfect. Because 131 is presumably directionally ...
263
Regulation of epithelial morphogenesis by Drosophila Rho GTPase and Abl kinase
... I evaluated the role of the small GTPase Rho1 as an adherens junction regulator in Drosophila and determined whether, as in cultured mammalian cells, Drosophila Rho1 is regulated by the [r] ...
160
Regulation of localization and function of the Rho family small GTPase, Rnd3
... of protein-protein ...a specific protein interacts have particular significance for the biological functions of that ...the specific signaling pathways in which it participates, and the ...
207