Ribosome Assembly
ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria
... in ribosome assembly, with an rsgA deletion strain, or a strain inactivated for GTPase activity, displaying decreased growth, a decrease in the amount of mature 70S ribosomes and an increased level of ...
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Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex
... splicing, ribosome biogenesis, translation, and mRNA decay ...the assembly of the West Nile virus in a RNA helicase activity-dependent manner ...the assembly of the large (60S) ribosomal subunit ...
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The Small-Subunit Processome Is a Ribosome Assembly Intermediate
... present results that expand upon these studies and validate the role of seven nonribosomal proteins in pre-rRNA processing. During the original purification, analysis was limited to pro- teins whose peptide sequences ...
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Disruption of rimP-SC, encoding a ribosome assembly cofactor, markedly enhances the production of several antibiotics in Streptomyces coelicolor
... RimP affects the translational efficiency and fidelity in E. coli As a ribosome assembly cofactor, RimP may affect transla- tional efficiency. Thus, the translational accuracy was measured using the mutated ...
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The impact of the stringent response on TRAFAC GTPases and prokaryotic ribosome assembly
... of ribosome biogenesis and function, including ribosomal RNA (rRNA) transcription, 70S assembly and protein translation, are negatively impacted upon induction of a nutrient stress-sensing signalling ...
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Ribosomal Protein Rps26 Influences 80S Ribosome Assembly in Saccharomyces cerevisiae
... IMPORTANCE Rps26 is an essential protein of the eukaryotic small ribosomal sub- unit. Previous experiments demonstrated an interaction between the eukaryote- specific Y62–K70 segment of Rps26 and the 5= untranslated ...
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Endurance training lowers ribosome density despite increasing ribosome biogenesis markers in rodent skeletal muscle
... (Nop56), ribosome assembly (Ncl), ribosome processing from the pre-45S rRNA transcript (28S rRNA and 18S rRNA) and nuclear ribosome export (Npm1 and Fbl) in order to examine if these targets ...
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The ribosome returned
... the ribosome are assembled into a complex that has an aminoacylated initiator tRNA and an mRNA bound, ready to make the first peptide bond of a protein ...the ribosome assembly dismantled so that its ...
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Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae
... mutiple assembly steps is blocked, a common substrate is not immediately ...ceosome assembly or pre-mRNA splicing fails to occur prop- erly (Burgess and Guthrie 1993; Staley and Guthrie ...the ...
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Ribosome Profiling of Synechocystis Reveals Altered Ribosome Allocation at Carbon Starvation
... that ribosome assembly may occur upstream of the classical SD- dependent ribosome assembly ...the ribosome release factor (sll0145) (Table S2) is consistent with increased UTR read ...
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Mechanism of ribosome translation through mRNA secondary structures
... A ribosome is a macromolecular machine responsible for the synthesis of protein chains by a process called translation, where the sequence of the amino acid residues is encoded by that of codons in messenger RNA ...
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Structure based hypothesis of a mitochondrial ribosome rescue mechanism
... It is exactly between Thr-115 and Gly-116, two resi- dues interacting with both the stop codon and the ribo- somal decoding center, that the RT insertion has taken place. Whereas the threonine side chain (T-RTi) was ...
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The influence of ribosomal proteins on the action of ribosome inactivating proteins
... The functional role of ribosomal RNA with its highly conserved structures, has been identified for containing: the decoding site, the peptidyl transferase active centre, the binding site[r] ...
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Protein folding on the ribosome studied using NMR spectroscopy
... To achieve selective isotopic labeling of the RNCs in vivo, the culturing conditions of the E. coli are manipulated, such that dur- ing growth a medium is used which permits the production of unlabeled ribosomes. The ...
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Structure and mechanism of 16S rRNA in the ribosome studied by photocrosslinking
... The small number of crosslinks observed here in the s 4 U-substituted and irradiated 16S rRNA indicates a restricted pattern of crosslinking in the 30S subunit. When the same s 4 U-containing 16S rRNA was irradiated ...
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The Ribosome Binding Site of Hepatitis C Virus mRNA
... fingerprint (1, 3), the sequence of each purified 80S ribosome- protected fragment was determined. The termini of these se- quences identified points of RNase cleavage within, or at the borders of, HCV RBS ...
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Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling
... There are many other examples showing different patterns of associations between translation of the uORFs with their main CDS regions. The potential underlying mechanisms are certainly worth further investigations. ...
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Mutational Analysis of the Bovine Hepacivirus Internal Ribosome Entry Site
... hepaciviruses has been recently proposed which included the suggestion to allocate the BovHepVs to the species Hepacivirus N (2, 3). HCV is one of the leading causes for chronic hepatitis, liver cirrhosis, and ...
13
Slowing ribosome velocity restores folding and function of mutant CFTR
... Using ribosome profiling, we found that rates of translation initiation and elongation were markedly slowed by RPL12 ...interdomain assembly, and baseline open-channel ...of ribosome velocity ...
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Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer
... highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain [11]. It has been shown to play an important role in pro- collagen biosynthesis in secretory tissues and in ...
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