RIG-I
Paramyxovirus V Proteins Interact with the RIG-I/TRIM25 Regulatory Complex and Inhibit RIG-I Signaling
... (50). RIG-IN, RIG-IC, and full-length RIG-I constructs were described elsewhere (6, ...constructs). RIG-I, TRIM25, and the MAVS protein were amplified from template DNA described ...
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RIG I ATPase Activity and Discrimination of Self RNA versus Non Self RNA
... of RIG-I with or without dsRNA and a non-hydrolyzable form of ATP, the transition from autore- pressed to activated RIG-I involves closure of its helicase domains upon binding dsRNA and ATP, ...
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RIG-I contributes to the innate immune response after cerebral ischemia
... Since RIG-I protein expression is increased by ischemia in astrocytes ...in RIG-I and IFN-α at 3 and 4 h after OGD in astrocytes in ...type I IFNs such as ...
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Distinct RIG-I and MDA5 Signaling by RNA Viruses in Innate Immunity
... gene I (RIG-I) and melanoma differentiation- associated gene 5 ...for RIG-I, MDA5, and interferon promoter-stimulating factor 1 ...for RIG-I and MDA5 were ...whereas ...
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Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction
... against RIG-I (SC-48932), rabbit polyclonal antibodies against IRF-3 (SC-9082; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies against phospho-IRF-3 (Ser396) (4947S; Cell Signaling ...
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Cytoplasm and Beyond: Dynamic Innate Immune Sensing of Influenza A Virus by RIG-I
... on RIG-I for IFN induction, IAV differs from most RNA viruses in a way that its RNA synthesis takes place in the cell ...of RIG-I in IFN induc- tion has only been recently ...of ...
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Murine Coronavirus Induces Type I Interferon in Oligodendrocytes through Recognition by RIG-I and MDA5
... found on cells committed to the oligodendrocyte lineage as well as mature oligodendrocytes; and the anti-GC antibody, which recognizes the protein ex- pressed specifically by mature oligodendrocytes (43). The N20.1 cells ...
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Identification of a Natural Viral RNA Motif That Optimizes Sensing of Viral RNA by RIG I
... the RIG-I-like receptors (RLRs) RIG-I (retinoic acid-inducible gene I) and MDA5 (melanoma differentiation-associated protein 5) bind viral PAMPs present in the infected cell and ...
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Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I
... type I interferon ...on RIG-I involved in the signaling cascade for type I IFN production, leading to degradation of RIG-I upon bind- ...cleave RIG-I (33, 40), ...
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Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by SEC14L1
... with RIG-I, and had little effect on reducing the activation of the IFN-  and ISRE promoters induced by RIG-I-CARD ...with RIG-I and inhibitory func- ...the RIG-I- ...
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RIG I expression in perifascicular myofibers is a reliable biomarker of dermatomyositis
... Perifascicular RIG-I expression was found in 50% of patients with DM and in only 11% of patients with non- DM, demonstrating that this marker is significantly asso- ciated with DM (p < ...addition, ...
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Hijacking of RIG-I Signaling Proteins into Virus-Induced Cytoplasmic Structures Correlates with the Inhibition of Type I Interferon Responses
... the RIG-I/TRIM25 complex to cytoplasmic ...BiFC RIG-I/TRIM25 (left) or BiFC RIG-I/MAVS (right) construct pairs along with an empty plasmid or a plasmid carrying SFTSV ...
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Adenovirus Virus-Associated RNAs Induce Type I Interferon Expression through a RIG-I-Mediated Pathway
... or RIG-I siRNA ...and RIG-I was examined at 24 h posttransfection by ...of RIG-I knockdown on VAI- and VAII-induced IFN-  ...or RIG-I ...type I IFN ...and ...
6
Conventional Protein Kinase C-α (PKC-α) and PKC-β Negatively Regulate RIG-I Antiviral Signal Transduction
... type I in- terferons (IFN-␣/) is the key to a successful innate immune re- sponse to viral ...gene I (RIG-I)-like receptors (RLRs) (15, ...by RIG-I and melanoma ...
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Inhibition of Ongoing Influenza A Virus Replication Reveals Different Mechanisms of RIG-I Activation
... whether RIG-I activation in response to NP-free reconstitution could be attributable to these aberrant replication ...affecting RIG-I ...
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RIG-I Mediates an Antiviral Response to Crimean-Congo Hemorrhagic Fever Virus
... by RIG-I, an RLR involved in the detection of viruses producing 5=ppp or 5=pp ...recognition, RIG-I and MAVS mediated the upregula- tion of type-I IFN and ISGs in response to virus ...
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A Sendai Virus-Derived RNA Agonist of RIG-I as a Virus Vaccine Adjuvant
... viruses. RIG-I activation leads to the production of an appropriate cytokine and chemokine cocktail that stimulates an antiviral state and drives the adaptive immune system toward an efficient and specific ...
11
Nucleotide Sequences and Modifications That Determine RIG-I/RNA Binding and Signaling Activities
... through RIG-I, we compared the ability of the poly(U/ UC) RNA to induce IFN-  in wild-type and RIG-I knockout ...in RIG-I knockout MEFs, demonstrating that RIG-I ...
11
Arenaviral Nucleoproteins Suppress PACT-Induced Augmentation of RIG-I Function To Inhibit Type I Interferon Production
... Coimmunoprecipitation assay. HEK293T cells were grown overnight to 60% to 80% confluence in 10-cm tissue culture dishes in complete DMEM supplemented with 1% penicillin-streptomycin and 10% FBS. By using a standard ...
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Mismatches in the Influenza A Virus RNA Panhandle Prevent Retinoic Acid-Inducible Gene I (RIG-I) Sensing by Impairing RNA/RIG-I Complex Formation
... vivo RIG-I binding. 293T cells were first transfected with pEF-Bos-RIG-I (2 g) for 24 h and then transfected with 2 g of biotinylated perfect cRNA ...RNA-bound RIG-I. ...
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