RING E3 ubiquitin ligases
The Poxviral RING Protein p28 Is a Ubiquitin Ligase That Targets Ubiquitin to Viral Replication Factories
... the RING domain of ICP0 catalyzed the formation of multiubiquitin adducts in the presence of UbcH5a, whereas GST was inactive ...the RING domain was truncated or lacked crucial cysteines ...multiple ...
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Herpes Simplex Virus Type 1 Immediate-Early Protein ICP0 and Its Isolated RING Finger Domain Act as Ubiquitin E3 Ligases In Vitro
... single ubiquitin molecule to a lysine residue encoded by the intron sequences ...the E3 ligase activity, a plasmid was constructed that expresses GST-linked ICP0-241, a protein containing only residues ...
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Cullin-RING ubiquitin ligases: global regulation and activation cycles
... the RING finger motif of Rbx1 abolishes neddylation in vitro ...subunit E3 for the neddylation reaction, although it is possible that Rbx1 is the predominant E3 and DCN1 is a ...
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The Specificities of Kaposi's Sarcoma-Associated Herpesvirus-Encoded E3 Ubiquitin Ligases Are Determined by the Positions of Lysine or Cysteine Residues within the Intracytoplasmic Domains of Their Targets
... (16). RING-CH, RING finger domain; Tyr, tyrosine-based endocytosis motif; SH3B, potential SH3 binding site; DE1 and -2, acidic domains 1 and 2; CM, conserved ...
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Genetic and molecular characterization of Roc-Cullin interactions in Drosophila melanogaster E3 ubiquitin ligases
... spermatid differentiation [18]. Consequently, Roc1b mutants are male sterile [5]. Interestingly, the BNAR chimera, which binds well to Cul3, effectively rescues Roc1b mutant male sterility. This is consistent with our ...
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Integration of Fungus Specific CandA C1 into a Trimeric CandA Complex Allowed Splitting of the Gene for the Conserved Receptor Exchange Factor of CullinA E3 Ubiquitin Ligases in Aspergilli
... The ubiquitin-proteasome pathway includes the dynamic interplay of the substrate receptor exchange factor CandA and three macromolecular multiprotein complexes, SCF E3 ubiquitin RING ligase, ...
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Regulation of cell fate determination by Skp1 Cullin1 F box (SCF) E3 ubiquitin ligases
... Abbreviations used in this paper: APC/C, anaphase promoting complex/ cyclosome; Arm, armadillo; bHLH, basic helix-loop-helix; -TRCP, - transducin repeat containing protein; Ci, Cubitus interruptus; CKI, cyclin ...
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Arabidopsis BRUTUS-LIKE E3 ligases negatively regulate iron uptake by targeting transcription factor FIT for recycling
... different E3 ligases that are up-regulated dur- ing Fe deficiency (4, 14, 15) is a small gene family encoding E3 ubiquitin ligases with N-terminal hemerythrin ...motif-containing ...
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The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases
... catalytic RING domain is responsible for regulating TNFα-induced apoptosis [28]; thus, peptide-based inhibition can be a very specific way to block TNF signaling while preserving Pellino3 function in other immune ...
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Atlas on substrate recognition subunits of CRL2 E3 ligases
... Cullin2-type ubiquitin ligases belong to the Cullin-Ring Ligase (CRL) family, which is a crucial determinant of proteasome-based degradation processes in ...Cullin2-type ubiquitin ...
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Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2
... growth throughout its life cycle. Most aspects of polarized growth in budding yeast arise from a precise arrangement of the cortical actin cytoskeleton during the cell cycle. Three main actin structures can be found in ...
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E3 ubiquitin ligases and their control of T cell autoreactivity
... conserved RING) functions as a dominant negative mutant capable of inhibiting endogenous GRAIL function and blocking the development of anergy ...H2N2 RING mutants also fail to suppress IL-2 secretion in ...
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The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions
... Cullin-RING E3 ubiquitin ligase complexes; Cul2: Cullin-2; Rbx1: RING protein; ECS: Elongin B and C-Cul2 or Cul5-SOCS box protein; VHL: von Hip- pel–Lindau; HIFα: α subunits of hypoxia ...
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Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases
... Cullins are posttranslationally modified by the ubiquitin-like protein Nedd8. Neddylation of Cul3 occurs near the Rbx1 binding site and is required to form the binding interface that recruits the E2-conjugating ...
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Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3
... muscle RING finger 1 (MuRF1) and MuRF3 act as E3 ubiquitin ligases that cooperate with the E2 ubiquitin–conjugating enzymes UbcH5a, -b, and -c to mediate the degradation of β/slow ...
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Virus-Specific Effects of TRIM5αrh RING Domain Functions on Restriction of Retroviruses
... disassembly of the capsid (27). Some investigators have reported that proteasome inhibitors relieve the TRIM5␣-mediated block of viral reverse transcription but not infection, implying a two-step restriction mechanism ...
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Role of TRIM5α RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus
... TRIM5 RING E3 ubiquitin ligase activity (data not ...the ubiquitin-charged E1 enzyme and other potential cellular contaminants, the anti-HA Western blot was used for quantification of the ...
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Putative E3 Ubiquitin Ligase of Human Rotavirus Inhibits NF κB Activation by Using Molecular Mimicry To Target β TrCP
... intact RING domain, is sufficient to target SCF -TrCP and that SA11-4F and OSU NSP1 proteins conserve an architecture and mechanism similar enough to permit this transfer of ...
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Glasmacher, Elke (2010): Roquin binds to ICOS mRNA for P body-dependent and miRNA-independent posttranscriptional gene silencing. Dissertation, LMU München: Fakultät für Biologie
... the full length Roquin and MNAB proteins from our adenoviral vectors. Interestingly, cDNA-based MNAB expression not only resulted in the full-length protein, but also in a shortened version, which was detected by ...
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IRF3 Inhibition by Rotavirus NSP1 Is Host Cell and Virus Strain Dependent but Independent of NSP1 Proteasomal Degradation
... and E3 ligases (2, 7, 19, 24, 33, 42, 50, 51, 54, ...N-terminal RING finger domain of NSP1 reportedly result in increased NSP1 expression and a reduced IRF3 degradation function ...the RING ...
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