RK13 Cells
Evaluation of Changes in Actin Filaments of RK13 Cells Infected with Malassezia pachydermatis
... In conclusion, we were able to establish an infection model of RK13 cells by M. pachydermatis . Yeasts adhered themselves to culture cells forming grape-like colonies. The more relevant findings ...
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Reverse Genetics Analysis of Poxvirus Intermediate Transcription Factors
... (13). RK13 cells were infected with 10 PFU/cell of WT, vA8⌬, or vA23⌬ VACV, the cell pellets FIG 2 Replication of A8R and A23R gene deletion mutant viruses in the complementing RK13-A8/A23 cell ...to ...
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The Vaccinia Virus K1L Gene Product Inhibits Host NF-κB Activation by Preventing IκBα Degradation
... of RK13 cells individually infected with WR, MVA, or MVA/K1L were probed for IB␣ by immunoblotting, IB␣ was readily detected in WR- and MVA/K1L-infected ...and RK13 cells. (A and B) 293T ...
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Pathogenic Mutations within the Hydrophobic Domain of the Prion Protein Lead to the Formation of Protease-Sensitive Prion Species with Increased Lethality
... mutant cells), it was difficult to conclu- sively determine if prions were in fact being ...infection. Cells were lysed via passage through small-gauge needles and the resulting homogenate inoculated into ...
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Impact of ETIF Deletion on Safety and Immunogenicity of Equine Herpesvirus Type 1-Vectored Vaccines
... (A) RK13 cells were infected with an MOI of 1 of the indicated viruses or transfected with the indicated plasmids (pc-syngag or ...Infected cells were harvested 24 h postinfection, and transfected ...
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The Native Form and Maturation Process of Hepatitis C Virus Core Protein
... Western blot analysis of HCV core protein. The protein samples prepared from RVV-infected and DNA-transfected RK13 cells and in vitro translation reaction were separated by 10 to 20% gradient and SDS–15% ...
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The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids
... UL25F-infected RK13 cells, no extracellular virions or nucleocapsids in the cytoplasm were detectable ...UL25F-infected RK13 cells, numerous capsidless tegu- ment-containing particles (L, or ...
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Highly Efficient Induction of Protective Immunity by a Vaccinia Virus Vector Defective in Late Gene Expression
... complementing cells as effi- ciently as does the wt ...in RK13 cells. The cells were infected with an MOI of ...complementing cells, approximated that of the wt virus in RK13 ...
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Statin induced inhibition of the Rho signaling pathway activates PPARα and induces HDL apoA I
... HepG2 cells (Figure ...site. RK13 cells were transfected with a luciferase reporter vector containing six copies of the DR1 site cloned in front of the thymidine kinase promoter in pGL3 ...
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A Pseudorabies Virus Recombinant Simultaneously Lacking the Major Tegument Proteins Encoded by the UL46, UL47, UL48, and UL49 Genes Is Viable in Cultured Cells
... of RK13 cells (18) with mutated bacterial arti- ficial chromosome DNA and a plasmid containing the authen- tic gG gene of PrV ...of RK13 cells with the resulting plasmid pBl-B1B and virion DNA ...
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Identification of a 709-Amino-Acid Internal Nonessential Region within the Essential Conserved Tegument Protein (p)UL36 of Pseudorabies Virus
... complementation, RK13 cells were transfected either with pcDNA3 as a negative control, pcDNA-UL36 as a positive control, or the respective truncated versions of ...of RK13 cells with the ...
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The Pseudorabies Virus UL11 Protein Is a Virion Component Involved in Secondary Envelopment in the Cytoplasm
... infected cells, PrV UL11-specific labeling was predominantly detectable in speck- les in the cytoplasm indicative of Golgi or Golgi-derived mem- branes and at the plasma ...nonpermeabilized cells (data not ...
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Functional Analysis of the Pseudorabies Virus UL51 Protein
... (PSEK) cells in Eagle’s minimum essential medium supple- mented with 10 or 5% fetal calf serum, ...UL51-expressing cells, pcDNA-UL51 was prepared after PCR amplification of the complete UL51 ORF (see ...
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Nuclear Envelope Breakdown Can Substitute for Primary Envelopment-Mediated Nuclear Egress of Herpesviruses
... PrV-infected cells, genome-less capsids are only rarely ob- served in the cytoplasm, indicating a quality control at the stage of nuclear egress ...infected cells with NEBD numerous capsid forms lacking DNA ...
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Pseudorabies Virus Glycoprotein K Requires the UL20 Gene Product for Processing
... of RK13 cells by SuperFect (Qiagen, Hilden, Germa- ...designated RK13-gK1-3 (complete gK ORF) and RK13-gK2-3 (gK from the second possible translation ...
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The UL7 Gene of Pseudorabies Virus Encodes a Nonessential Structural Protein Which Is Involved in Virion Formation and Egress
... reverse (⫺48) primers (New England Biolabs). The obtained PCR product was used for Red recombinase-mediated mutagenesis of the BAC pPrV-⌬gB in E. coli as described previously (11, 32). After isolation of kanamy- ...
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Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.
... 293 cells as complementing ...These cells, however, were not ideal for supporting growth of vaccinia ...in RK13 cells (data not shown). In addition, plaques on 293 cells were small and ...
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Effects of Deletion or Stringent Repression of the H3L Envelope Gene on Vaccinia Virus Replication
... infected cells with the WR-derived virus vT7lacOI (1), already carrying the repressor and bacteriophage T7 RNA polymerase ...infected cells were then trans- fected with a plasmid transfer vector (44) ...
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Embryonic stem cells differentiate into insulin producing cells without selection of nestin expressing cells
... mesenchymal cells, called pancreatic stellate cells (Lardon et al ...progenitor cells in ...expressing cells were found to contribute mainly to the exocrine lineage (Delacour et al ...
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A review paper on stem cells
... stem cells (ESC) have unleashed new avenues in the field of developmental biology and emerged as a potential tool to understand the molecular mechanisms taking place during the process of differentiation from the ...
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