RNA aptamer
Antimicrobial peptide LL-37 facilitates intracellular uptake of RNA aptamer Apt 21-2 without inducing an inflammatory or interferon response
... anti-IL-17A RNA aptamer Apt 21-2 has also been shown to effectively bind IL-17A, and we and others have previously illustrated that Apt 21-2 may be suitable for use in treating psoriatic plaques (24, ...
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Distinguishing closely related amyloid precursors using an RNA aptamer
... F-modified RNA aptamer able to distinguish between these very similar ...N30 RNA pool yielded an aptamer (B6) that binds h  2 m with an EC 50 of ⬃200 n M ...
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IL-17A RNA aptamer: possible therapeutic potential in some cells, more than we bargained for in others?
... of RNA aptamer uptake by keratinocytes and what cellular mechanisms are at ...the RNA aptamer into these ...method. RNA aptamers targeting inflammatory signalling pathways could present ...
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UCLA1, a Synthetic Derivative of a gp120 RNA Aptamer, Inhibits Entry of Human Immunodeficiency Virus Type 1 Subtype C
... UCLA1 RNA aptamer was examined for its an- tiviral activity against HIV-1 subtype C ...the aptamer binding sites revealed 8 res- idues that modulated neutralization resistance to the ...The ...
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An RNA aptamer targets the PDZ-binding motif of the HPV16 E6 oncoprotein.
... no aptamer control) is shown graphically below the ...of aptamer (lane ...no aptamer control) is shown graphically below the ...no aptamer control) is shown graphically below the gel, however, ...
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Effect of a calcitonin gene-related peptide-binding L-RNA aptamer on neuronal activity in the rat spinal trigeminal nucleus
... to mouse/rat αCGRP has been used to elucidate strain dif- ferences in rats regarding CGRP release and behavioral re- sponses to noxious heat [32]. It was furthermore reported to reduce CGRP-dependent vasodilatation and ...
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Aptamer-based therapeutic approaches to target cancer wtem cells
... a RNA aptamer against EGFR (E07) to escort GEM to pancreatic cancer ...EGFR aptamer to create a nucleic acid compound with two functional domains composed of EGFR aptamer and GEM-containing ...
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Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review
... function, RNA aptamer motifs have been widely modi- fied to bind adaptor domain fused ...between RNA aptamers and their corresponding protein adap- tors, the single-strand RNA containing ...
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Inhibition of Bone Marrow-Derived Mesenchymal Stem Cells Homing Towards Triple-Negative Breast Cancer Microenvironment Using an Anti-PDGFRβ Aptamer
... nuclease-resistant RNA aptamer against platelet-derived growth factor receptor β (PDGFRβ) causes the inhibition of receptor-dependent signaling pathways thus drastically hampering BM-MSC recruitment towards ...
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Identification of osteopontin dependent signaling pathways in a mouse model of human breast cancer
... RNA aptamer and systematic evolution of ligands by exponential enrichment (SELEX): The SELEX selection procedure was utilized to isolate candidate OPN aptam- ers, as described ...the aptamer pool's ...
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Aptamers—A Promising Approach for Sensing of Biothreats Using Different Bioinformatics Tools
... tion of both sequences and structure. Generally the pro- duct of last cycle of SELEX was taken and sequenced and examined for the possible sequence motifs using different motif binding programs [14,15]. Binding assays ...
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Claypool_unc_0153D_15736.pdf
... 2’-F RNA aptamer, ...either aptamer M9-5 or SQ-2. With the M9-5 aptamer, there were technical difficulties in synthesizing the RNA aptamer by ...the RNA transcription was ...
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Clinical potential of oligonucleotide therapeutics against pancreatic cancer
... 2'-fluropyrimidine RNA aptamers P19 and P1, which were selected by SELEX, resulting in tumor-suppressive effects ...hybridized aptamer-drug conjugates that involved pancreatic cancer- specific RNA ...
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RNA aptamers specifically interact with the prion protein PrP.
... preparation. RNA aptamer motifs I, II, and III were synthesized from individual aptamer clones by in vitro transcription with T7 RNA polymerase, or we amplified the relevant cDNA coding for ...
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HAPIscreen, a method for high-throughput aptamer identification
... digoxigenin-tagged aptamer (R06) and a biotinylated target RNA hairpin ...(TAR) RNA element of HIV-1 (biot-TAR), 5 ’ end-extended TAR (rA-TAR), RNA aptamer R06 (dig-R06), domain II of ...
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<p>Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus</p>
... the RNA aptamer by in vitro transcription (37°C 3 ...located RNA band was cut from the gel and ethanol precipitated using the crush and soak method, and the concentration was determined ...
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Theophylline IMAGEtags (Intracellular Multi Aptamer Genetic tags): the development and evaluation of an RNA reporter system based on the theophylline aptamer
... an RNA tag and reporter gene using the RNA aptamer for ...An RNA reporter could provide a more direct measure of transcriptional activity than enzyme based reporter genes, and potentially ...
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Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy
... strategy. RNA aptamer A10 has been used as a ligand, targeting prostate cancer cells that express prostate-specific membrane antigen ...second-generation RNA aptamer, ...
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Template Recognition Mechanisms by Replicase Proteins Differ between Bipartite Positive-Strand Genomic RNAs of a Plant Virus
... viral RNA fragments under our experimen- tal conditions ...(nt) RNA aptamer that binds to streptomycin with high ...viral RNA fragments that covered the entire RCNMV genome sequence ...
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Terahertz Detection of Zika Viruses
... studied using terahertz spectroscopy. In most sensing applications, structures to focus the terahertz electromagnetic signal in the form of bowtie antenna or plasmonic structures are used. Viruses are usually detected ...
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