RNA aptamers
RNA aptamers specifically interact with the prion protein PrP.
... selected RNA aptamers in the clones sequenced were as follows: for motif I, 10%; for motif II, 15%; for motif III, ...selected RNA aptamers did not contain any G quartet ...these ...
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RNA aptamers selected to bind human immunodeficiency virus type 1 Rev in vitro are Rev responsive in vivo.
... RNA aptamers (binding sequences) that can interact tightly and specifically with the human immunodefi- ciency virus type 1 Rev protein have previously been selected from random sequence ...Two ...
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Random mutagenesis of NS1 protein of influenza a H1N1 and docking of RNA aptamers to wild type and mutant NS1 proteins
... to RNA aptamers to examine whether the structures of mutants affect docking properties as well as to select RNA aptamer for high affinity binding to NS1A ...
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First report of in vitro selection of RNA aptamers targeted to recombinant Loxosceles laeta spider toxins
... acid aptamers over- come these limitations and are thus a promising alter- native to ...antibodies. RNA or DNA oligonucleotides that have been selected for their ability to bind to other mol- ecules with ...
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Inhibition of Hepatitis C Virus (HCV) Replication by Specific RNA Aptamers against HCV NS5B RNA Replicase
... avid RNA aptamers consisting of 2=-hydroxyl- or 2=-fluoropyrimidines against hepatitis C virus (HCV) NS5B replicase, an enzyme that is essential for HCV ...These aptamers acted as potent decoys to ...
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Selection of RNA aptamers that bind HIV 1 LTR DNA; strand invaders
... between RNA and duplex DNA, we selected for RNA aptamers that can bind to the long terminal repeats (LTRs) of human immunodeficiency virus type 1 ...selected aptamers were classified into four ...
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Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers.
... characterized RNA aptamers selected to FMDV 3D pol that were able to inhibit the activity of the enzyme in vitro (Ellingham et ...the aptamers were termed 47 and ...form RNA–protein fibrils in ...
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RNA Aptamers Directed to Human Immunodeficiency Virus Type 1 Gag Polyprotein Bind to the Matrix and Nucleocapsid Domains and Inhibit Virus Production
... anti-Gag RNA aptamers to inhibit HIV-1 replication. In vitro, RNA aptamers raised against an HIV-1 Gag protein, lacking the N-terminal myristate and the C-terminal p6 (DP6-Gag), could bind to ...
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Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase
... individual aptamers belonging to each class was conserved, with the common sequence motifs as part of analogous struc- tural ...selected aptamers of A and B classes are reported in ...selected ...
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Characterization of In Vitro Selected RNA Aptamers for the RB69 RegA Translational Repressor Protein and Optimization of in vitro Selection Utilizing Complex Targets
... Nucleic acid selections are highly successful inside the laboratory, however, this success quickly coorelates into failure if the efficacy of the selected molecules is poor due to quick degradation, in real world ...
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Targeting the Variable Surface of African Trypanosomes with Variant Surface Glycoprotein-Specific, Serum-Stable RNA Aptamers
... Lastly, we verified that the N-Asn linked sugar side chain modifications of the sVSG protein do not contribute to the binding of the RNAs. Deglycosylated sVSG interacts with the aptamer RNAs indistinguishable from ...
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The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and its Perturba-tion by RNA Aptamers
... of aptamers from the endocytic ...of aptamers, transfection of siRNAs using cationic lipids, nanoparticles or cell-type-specific delivery reagents have shown that siRNAs are traf- ficked to the endosomal ...
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Oligonucleotide based systems: DNA, microRNAs, DNA/RNA aptamers
... Figure 1. Biogenesis and function of microRNAs. MicroRNAs are transcribed by RNA polymerase II into primary transcripts called pri‐miRNAs which fold into a stemloop structure. They are trimmed in the nucleus by a ...
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Aptamers in Education: Undergraduates Make Aptamers and Acquire 21 st Century Skills Along the Way
... anti-CIAP RNA aptamers with nM affinities for ...anti-CIAP RNA aptamers 4-3, 4-9, 3-6, and minimized 3-1 variant have the following Kds, respectively: 5 nM, ...anti-CIAP aptamers after ...
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1 DNA Aptamers for Functionalisation of DNA
... Figure 3. Examples of aptamer use in controlling DNA origami structural change. (A) Schematic for designing aptamer-based fluorescent reporters that function by switching structures from DNA/DNA duplex to DNA/target ...
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<p>Aptamers as Versatile Ligands for Biomedical and Pharmaceutical Applications</p>
... produced RNA aptamers that speci fi cally bound to mycolactone for diagnosis of buruli ulcer (BU) using the SELEX ...using RNA aptamers was feasible. Recently, aptamers have received ...
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Cross-Clade Inhibition of Recombinant Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus SIVcpz Reverse Transcriptases by RNA Pseudoknot Aptamers
... There is a growing appreciation that evaluation of the utility of novel antiviral compounds must include an assessment of their efficacies against RTs from phylogenetically diverse HIV strains. Although some inroads have ...
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The selection of aptamers specific for membrane molecular targets
... [1]. RNA aptamers are single-stranded oligonucleotides composed of ...cellular RNA aptamers, functioning for example as binding sites for free amino acids on the Tetrahymena self-splicing rRNA ...
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Neutralizing DNA Aptamers against Swine Influenza H3N2 Viruses
... Moreover, aptamers can be easily and inexpensively synthe- sized without batch-to-batch variation (37, ...antibodies, aptamers can also be labeled with reporter molecules such as fluorescein, biotin, and ...
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Aptamers—A Promising Approach for Sensing of Biothreats Using Different Bioinformatics Tools
... sensitivity. Aptamers are single stranded oligonucleotides binding species which are capable of tightly binding to their distinguishing ...design RNA aptamers for toxins of lectin ...
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