RNA binding
Identification of the RNA-Binding, Dimerization, and eIF4GI-Binding Domains of Rotavirus Nonstructural Protein NSP3
... the RNA binding properties of NSP3A and that NSP3C did not form dimers under nonre- ducing conditions (33) prompted a reevaluation of the pres- ence of disulfide bonds in NSP3A produced in rotavirus-in- ...
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Biochemical and functional characterization of human RNA binding proteins
... functional RNA elements sub- stantially expands the repertoire of regulatory components encoded in the human ...DNA binding proteins mostly culminates in effects on gene expres- sion levels, RBP function ...
226
Versatility of RNA-Binding Proteins in Living Cells
... canonical RNA binding domains, which include the RNA recognition motif, the heterogeneous ribonucleoprotein K homology domain, and C3H1 zinc-finger domain [1] Some of the proteins have previously ...
7
RNA-Binding Domain Proteins in Kinetoplastids: a Comparative Analysis
... have binding spec- ificity for G- or U-rich RNA (9), and UBP1 and UBP2 have been implicated in posttranscriptional control of gene expres- sion in both ...promote RNA stabilization, forming a ...
9
RBP38, a Novel RNA-Binding Protein from Trypanosomatid Mitochondria, Modulates RNA Stability
... RNA-binding proteins are involved in the synthesis, process- ing, transport, translation, degradation, and stabilization of RNA (7, 24). The mitochondrial genome of trypanosomatids is composed of ...
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Identification of RNA Binding Proteins and RNA Binding Residues Using Effective Machine Learning Techniques
... of RNA Binding Proteins (RBPs) and RNA Binding residues from sequence information alone is one of the most challenging problems in computational ...and RNA metabolism ...and RNA ...
78
OST-HTH: a novel predicted RNA-binding domain
... other RNA-binding domains with the classical and circularly permuted versions of the wHTH fold ...S19AE, RNA 2 ’ phosphotransferase, La, the ribosomal protein S10E, a domain of the archaeal-type ...
8
RNA binding properties of poliovirus subviral particles.
... immobilized RNA molecules select RNA-binding proteins from protein preparations (5, 14) was used to detect interactions between putative viral assembly intermediates and the poliovirus RNA ...
10
Functional characterization of the RNA binding protein RALY
... however, is the fact that a signi fi cant portion of the identi fi ed interactors is implicated in mRNA translational control. Our data suggest that RALY might have different functions in mRNA metabolism that need further ...
97
Ribonomic approaches to study the RNA-binding proteome
... an RNA target to beads or to the surface of a microtiter plate well, large protein libraries can be screened against the RNA target and amplified in a process called in vitro ...the RNA-binding ...
16
RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence
... the RNA double helix, dsRBDs have been later found to be sensitive to additional RNA ...A-form RNA helix, other RNA features, such as apical loops and nucleotide ...dsRBD binding is ...
21
Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture method
... assigned RNA-binding activity to all the members of the Alba- and the Whirly (WHY)-domain-containing protein ...with RNA in the RBPome of etiolated seedlings [34], which strongly suggest that these ...
21
Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3
... KH4 binding the respective GGC ...unspecific binding caused by protein ...assayed RNA binding of full-length IMP3 protein to an additional mutant derivative of the 101-mer RNA, ...
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Double-Stranded RNA Binding by Human Cytomegalovirus pTRS1
... dsRNA- binding and dimerization domains while the two domains of pTRS1 may be ...double-stranded RNA-binding proteins like pTRS1 that may be less abundant or ...
9
Structure of noncoding RNA is a determinant of function of RNA binding proteins in transcriptional regulation
... the binding to G-quadruplex RNA but also that to G- quadruplex DNA ...acids binding protein that selec- tively target the G-quadruplex ...the binding RGG domain of EWS to G-quadruplexes but ...
8
An investigation into the RNA binding protein UNR and its interactors
... to RNA to protein has been referred to as the Central Dogma of Molecular Biology although the author of this term later qualified this simplistic concept of unidirectional flow (Crick, 1970) following the ...
407
Sequence-Based Prediction of RNA-Binding Residues in Proteins
... the RNA and protein components of pro- tein–RNA complexes [ 52 ] ( see Subheading ...each RNA binding part- ...these RNA-binding ...partner RNA sequence as input. If the ...
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Ebola Virus VP30 Is an RNA Binding Protein
... by matrix-assisted laser desorption ionization–time of flight spectroscopy. The protein match searches for each of the four major bands visualized by SDS-PAGE (Fig. 2A) matched the EBOV VP30 with a protein score of 101 ...
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The fate of RNA and RNA binding proteins in Sindbis virus infection
... Not only does iCLIP improves the resolution, but by introducing random tags on the adapters it reduces the effect of the PCR amplification preference. However, due to the stringent experimental conditions involved in ...
111
RNA Bind-n-Seq: Quantitative Assessment of the Sequence and Structural Binding Specificity of RNA Binding Proteins
... of binding affinities, particularly when several RBP concentrations are used, enabling detection of weaker but significant motifs, such as GCACG for ...with RNA representing canonical motif, UGCAUG (Auweter ...
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