RNA extraction from infected human cells
Small RNA analysis in Sindbis virus infected human HEK293 cells
... of human embryonic kidney 293 cells (HEK 293) and the changes in cellular miRNA profiles by high throughput ...derived from the human and not from the virus ...derive from Dicer ...
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Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells.
... The adenovirus type 12-specific mRNA and the stable nuclear RNA from pro- ductively infected KB cells, early postinfection, from abortively infected BHK-21 cells, and from the adenovirus[r] ...
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Phagocytosis of Picornavirus-Infected Cells Induces an RNA-Dependent Antiviral State in Human Dendritic Cells
... of infected cells. Our experiments identified RNA within infected Vero cell preparations as the crucial component for both ISG upregulation and resistance against ...noninfected cells ...
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A Virus-Encoded RNA Polymerase Purified from Baculovirus-Infected Cells
... host RNA poly- merase II for transcription of viral ...DNA-dependent RNA polymerases, and their need for a virus-encoded enzyme is usually attributed to the fact that they replicate in the cytoplasm and ...
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Characterization of polysome-associated RNA from influenza virus-infected cells.
... To assure saturating conditions, increasing concentrations (1 x, 2 x, 3 x, etc.) of unlabeled RNA species (either total cell RNA containing cRNA prepared as above, or unlabeled vRNA extr[r] ...
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Isolation and Properties of an RNA Polymerase from Influenza Virus-Infected Cells
... polymerase activity in fraction 4 from influenza virus-infected BHK21-F cells.. Batches of about 3 X 108 cells were harvested at each time point, and fractions were assayed for RNA polym[r] ...
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Kinetics of RNA synthesis in rotavirus infected cells
... d from b o v in e , c a n in e , fe lin e , simian and p o r c in e s o u r c e s (H o sh in o e t a l 1981, W yatt e t a l 1 9 8 2 ...le human R ota viu s b y g e n e re a ss ortm en t m ethods w h ere the ...
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Techniques for RNA extraction from cells cultured in starpeg heparin hydrogels
... the RNA. Both, the Norgen Total RNA Purification Plus kit and the Zymo Direct-zol RNA MiniPrep kit, yielded a good quantity of high-quality RNA suitable for cDNA synthesis and RT- ...to ...
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Rapid accumulation of measles virus leader RNA in the nucleus of infected HeLa cells and human lymphoid cells.
... RNA was also detected in total RNA isolated from measles virus-infected HeLa cells displaying 80 to 100% cell involve- ment; however, a time course for the accumulation of leader RNA was[r] ...
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Analysis of the In Vitro Product of an RNA-Dependent RNA Polymerase Isolated from Influenza Virus-Infected Cells
... Approximately 50% of the RNA synthesized in vitro remains associated with the 50 to 70S ribonucleoprotein complex containing polymerase activity; the remainder of the RNA polymerase prod[r] ...
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Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
... persistently infected cells were overlaid on uninfected cells, they readily fused ...cured cells prob- ably redistribute CD46 on their surfaces, reachieve the normal even distribution of ...
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Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells.
... periments with the R-FeLV cDNA were per- formed across polyribosome gradients to corre- late the binding of anti-FeLV to nascent virus- specific protein RNA within polyribosomes. The R-F[r] ...
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Transcription of the genome of adenovirus type 12. IV. Maps of stable late RNA from productively infected human cells.
... sized late in productive infection were deter- mined by saturation hybridization experi- ments, using specific restriction endonuclease fragments of Adl2 DNA.. We have also started.[r] ...
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Comparison of commercial RNA extraction kits for preparation of DNA free total RNA from Salmonella cells
... Salmonella RNA extraction. The greatest concentration of total RNA was obtained with the MasterPure kit; however, the sample contained high levels of genomic DNA, which interferes with invA mRNA ...
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Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells
... viral RNA colocalize along the rim of the ...nuclei from infected cells were, in fact, involved in viral RNA synthesis, we next attempted to localize de novo synthesized biotinylated ...
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Modulation of interleukin 1 beta RNA in monocytic cells infected with human immunodeficiency virus 1
... monocytic cells by using Northern blotting ...beta RNA even if the cells were infected with ...beta RNA as compared with their ...
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Isolation and Characterization of an RNA-Dependent RNA Polymerase from Black Beetle Virus-Infected Drosophila melanogaster Cells
... Crude lysates of black beetle virus (BBV)-infected cells of Drosophila mela-.. nogaster contain an RNA-dependent RNA polymerase activity not detectable in.[r] ...
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New procedure for isolation of Rous sarcoma virus-specific RNA from infected cells.
... cation of virus-specific RNA from chicken cells infected with avian sarcoma virus: identification of genome- length and subgenomic-length virol RNAs. An efficient mRNA dependent translat[r] ...
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Systematic identification of microRNA and messenger RNA profiles in hepatitis C virus-infected human hepatoma cells
... In this study, obtaining mRNA expression pro file together with miRNA pro filing cannot only provide a systematic data for miRNA regulatory network construction but also implicate the more accurate biological signi ficance ...
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Identification of Polysomal RNA in BHK Cells Infected by Sindbis Virus
... A portion of the RNA in the pooled peak of cores and polysomes and in the two peaks and pellet from the EDTA gradient was analyzed on polyacrylamide gels. The results (Table 1) showed th[r] ...
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