RNA extraction, library construction and sequencing
The impact of RNA sequence library construction protocols on transcriptomic profiling of leukemia
... that RNA-seq and fusion gene analysis can sensitively detect fusions despite some previous claims to the con- trary ...Sanger sequencing may not be sensitive to detect low expressed ...
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An improved protocol for small RNA library construction using High Definition adapters
... libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules. One ...
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Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
... bacterial RNA sequencing as the cDNAs corresponding to the contami- nating genomic DNA will result in inaccurate representa- tion of the expressed transcripts ...the RNA isolation procedures, evalu- ...
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Big data from small tissue extraction of high-quality RNA for RNA-Sequencing from different oilseed Brassica seed tissues during seed development
... of RNA, DNA and proteins from a single ...separate RNA from DNA and proteins and isopropanol for RNA precipitation 5 was attempted, the RNA obtained from GSSC using this methodology presented ...
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An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
... improve RNA extraction from various plant ...for RNA precipitation which was eliminated in the current method to reduce the time of RNA extraction from two days to 3 ...seed RNA ...
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Efficient extraction of RNA from various Camellia species rich in secondary metabolites for deep transcriptome sequencing and gene expression analysis
... quality RNA isolation. Based on the RNA isolated from flower buds, deep transcriptome sequencing of Camellia oleifera, Camellia chekiangoleosa and Camellia brevistyla were successfully carried ...454 ...
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Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina
... chloroform extraction step and replacing the isopropanol precipitation with ethanol pre- cipitation slightly improved the A 260/280 ratio while the yield remained ...the RNA yield. When the RNA ...
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mRNA Extraction from Gill Tissue for RNA-sequencing
... mRNA extraction from fish gills and ...as RNA-seq and ...involving RNA quality-control and quantification, RNA-seq library preparation and data ...in RNA-seq study design and ...
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Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
... DNA extraction, two library preparation kits and two NGS Illumina sequencing ...similar sequencing and assembly param- eters statistics with reference to sequencing outputs, in millions ...
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Combining DNA methylation and RNA sequencing data of cancer for supervised knowledge extraction
... Combined BRCA 1114 127 40530 542,5 THCA 515 65 40534 303,9 KIRP 292 53 40534 171,4 resulting classification models do not only consider the genes and their associated values for a single experiment, but both from gene ...
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CiteSeerX — A mammalian microRNA expression atlas based on small RNA library sequencing.
... For the new miRNA array platform, we synthesized a uni- versal miRNA standard from which we also prepared small RNA libraries that we cloned and sequenced. Relative clone frequencies for a given miRNA correlated ...
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Extraction of total RNA from single oocytes and single cell mRNA sequencing of swine oocytes
... the RNA with glyclogen in a 200 μl micro-centrifuge ...the RNA profile from single oocytes showed distinct peaks for 18S and 28S ribosomal ...the extraction of small RNAs from single oocytes, which ...
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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
... representative result is shown in Figure 3. Panel A shows the fabricated Gel-seq device; false color has been added to distinguish the different gel regions. Panel B shows a close up of four different separations used ...
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Single-cell RNA-sequencing of the brain
... After sequencing, quality control is performed on raw reads, aligned reads, and across the collection of cells to identify low quality ...bulk RNA-seq, include: per base sequence quality, sequence ...
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RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction
... transcriptome library (WTL) and gene specific library ...transcriptome library and gene specific library including their computational ...in RNA-seq ...specific library showed ...
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A comparison between whole transcript and 3' RNA sequencing methods using Kapa and Lexogen library preparation methods.
... two RNA-Seq methods using the classic whole transcript method (Trad-KAPA) and the 3′ method ...when sequencing depth ...the sequencing depths we ...
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16S Metagenomic Sequencing Library Preparation Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System
... for sequencing the variable V3 and V4 regions of the 16S rRNA ...for sequencing other regions with different region‐specific ...benchtop sequencing system, on‐board primary analysis, and secondary ...
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RNA CoMPASS: RNA Comprehensive Multi-Processor Analysis System for Sequencing
... Next-generation sequencing (NGS) technology or High-throughput sequencing (HTS) technology is rapidly becoming transformative in many areas of biology because NGS has significantly improved throughput and ...
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Discovering cancer-associated transcripts by RNA sequencing
... Ongoing maintenance and improvement of ChimeraScan will be essential if it is to remain a useful tool for gene fusion discovery. We have identified a number of current issues, feature requests, and enhancements and track ...
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Experimental design for single-cell RNA sequencing
... and longer yielded good quality cDNA. Depending on the FACS sorter, index sorting might be available and is highly recom- mended for downstream analysis and QC steps, as the transcrip- tome of individual cells can be ...
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