RNA segments
Functional Analysis of the Noncoding Regions of the Uukuniemi Virus (Bunyaviridae) RNA Segments
... Bunyaviridae RNA segments (L, M, S) in transcription, replication, and packaging was studied using the recently developed plasmid-driven RNA polymerase I minigenome system for Uukuniemi (UUK) virus, ...
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Rearranged Genomic RNA Segments Offer a New Approach to the Reverse Genetics of Rotaviruses
... rearranged segments 7 with a bovine RV as a helper virus, but whether this system can be extended to other rearranged segments or to different strains of helper viruses remains to be ...to segments ...
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Gene-Coding Assignments of Rotavirus Double-Stranded RNA Segments 10 and 11
... Extraction of dsRNA from purified virus, separation of the segments by polyacrylamide gel electrophoresis, and in vitro translation of individual denatured RNA segments were performed as[r] ...
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Mutational Analyses of Packaging Signals in Influenza Virus PA, PB1, and PB2 Genomic RNA Segments
... PB1 segments from diverse influenza A virus isolates (including all subtypes from various origins) were obtained from the NCBI influenza virus resource and ...
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Influenza B virus genome: assignment of viral polypeptides to RNA segments.
... Pulse-labeled infected cell polypeptides were separated on a 10 to 15% gradient polyacrylamide-SDS geL Lane 1, Uninfected cell polypeptides C; lane 2, polypeptides of B/Lee virus L-infec[r] ...
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Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis.
... 1 B that some isolates of T vaginalis were infected with an isometric virus containing a nonsegmented -5.5-kbp dsRNA genome.. However, close examination occasionally revealed the possibi[r] ...
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Capped and conserved terminal structures in human rotavirus genome double-stranded RNA segments.
... These terminal sequences, like those of reovirus and CPV, are capable of forming an end-to-end base-paired duplex structure: G ACC GmGC C CG CUGG Examination of terminal structures by S1[r] ...
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Transcriptional regulation of three double-stranded RNA segments of bacteriophage phi 6 in vitro.
... These two apparently different patterns produced in in vitro transcription presumably reflect the two distinct in vivo transcription patterns; i.e., i similar amounts of three single-str[r] ...
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Base Composition and Hybridization Studies of the Three Double-Stranded RNA Segments of Bacteriophage φ6
... Two possible explanations for this phenomenon are i the large dsRNA segment contained a lower G + C content than the two smaller segments and thus was the only segment that separated int[r] ...
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Roles of the Coding and Noncoding Regions of Rift Valley Fever Virus RNA Genome Segments in Viral RNA Packaging
... (FLUAV) RNA genome segments serve as minimal RNA replication signals (22), but the NCRs and the coding regions are both required for efficient genome packaging (5, 6, 13, 14, 16, 17, 20, 21, ...eight ...
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Generation of infectious RNA complexes in orbiviruses: RNA- RNA interactions of genomic segments
... genomic segments were needed to generate an infectious viral core and that in absence of certain segments ssRNAs were not packaged [7, ...through RNA-RNA interactions, thereby sorting the ...
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Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis
... Double-stranded RNA stranded RNA segments obtained from all three purified from reovirus type 3 was separated into reovirus serotypes have been resolved further by isolated distinct band[r] ...
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Rescue of Recombinant Thogoto Virus from Cloned cDNA
... gle-stranded RNA segments of negative polarity that are en- capsidated by the viral nucleoprotein (NP) and associate with the viral RNA polymerase complex to form ribonucleoprotein complexes (vRNPs) ...
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Effects of the Number of Genome Segments on Primary and Systemic Infections with a Multipartite Plant RNA Virus
... AMV RNA segments can achieve cell-to-cell movement (16–18), whereas for systemic movement, the formation of virus particles, each again encapsidating a single RNA segment, is required ...expressed ...
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Hierarchy among Viral RNA (vRNA) Segments in Their Role in vRNA Incorporation into Influenza A Virions
... negative-strand RNA segments. Although all eight segments must be present in cells for efficient viral replication, the mechanism(s) by which these viral RNA (vRNA) segments are ...
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Polyadenylation sites for influenza virus mRNA.
... The analysis described in this report demonstrates that in at least four of the eight RNA segments of the influenza virus genome, the tract of uridine residues common to each segment of [r] ...
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Polygenic control of neuroinvasiveness in California serogroup bunyaviruses.
... Studies of the sequential spread of virus after subcutaneous inoculation of 103 PFU into suckling mice indicated that reassortants with no attenuated RNA segments replicated to a high ti[r] ...
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Frequent Reassortments May Explain the Genetic Heterogeneity of Rotaviruses: Analysis of Finnish Rotavirus Strains
... gene segments of G1 and G4 rotavirus ...some RNA segments between these rotaviruses and the P[4], P[6], and P[9] G2 and G3 rotaviruses is not ruled ...other segments may be freer of restric- ...
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Two Unique RNA Species of the Nodavirus Black Beetle Virus
... MOORE2* Two-dimensional fingerprinting of RNase T1-derived oligonucleotides of the two individual RNA segments of the Nodavirus black beetle virus indicates that each RNA species possess[r] ...
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Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs
... negative-sense RNA genome which consists of the three segments L, M, and ...viral RNA segments in the antigenomic orientation, transcribed intracellularly by the T7 RNA polymerase and ...
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