RNA sequences for in vitro duplex study
In Vitro Selection of RNA Sequences That Mediate the Formation of Iron Oxide Nanoparticles
... using RNA to mediate the formation of materials is the ability to employ an in vitro selection approach (Scheme ...Nucleotide sequences that catalyze certain reactions or specifically bind to ...
101
5′-Terminal Sequences of Spring Viremia of Carp Virus RNA Synthesized In Vitro
... Compared with the results ob- tained previously for VSV Indiana termini (10, 11), it appears from the pH 3.5 DEAE-cellulose elution characteristics obtained for SVCV prod- uct labeled in[r] ...
11
Coding Sequences Enhance Internal Initiation of Translation by Hepatitis A Virus RNA In Vitro
... by RNA sequences and structure have been focused within the borders of the ...present study of a series of constructs retaining the HAV 59NCR ex- tending into various lengths of HAV coding ...
7
Hepatitis B Virus Reverse Transcriptase and ε RNA Sequences Required for Specific Interaction In Vitro
... left-side sequences here caused an even more severe defect than the right-side substi- tution in RT binding, although the same left-side substitution mutant was active in pgRNA packaging ...in vitro binding ...
10
Diverging Affinity of Tospovirus RNA Silencing Suppressor Proteins, NSs, for Various RNA Duplex Molecules
... antiviral RNA silencing mechanism in ...double-stranded RNA molecules, i.e., small interfering RNAs (siRNAs) and micro-RNA (miRNA)/miRNA* ...double-stranded RNA (dsRNA), whereas tomato yellow ...
13
Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... virus RNA-dependent RNA polymerase, purified from virion ribonucleoprotein particles and from which endogenous genomic RNA (vRNA) has been depleted by treatment with micrococcal nuclease, was used to ...
9
Polyadenylation of Influenza Virus mRNA Transcribed In Vitro from Model Virion RNA Templates: Requirement for 5′ Conserved Sequences
... Whether a trans-acting polymerase complex which is associ- ated with a 59 terminus but is not covalently linked to the template being transcribed is used for cRNA synthesis in vivo is unknown. Whatever the mechanism for ...
7
The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure.
... For the present study, we constructed a series of mutant RNAs which contain the reporter CAT gene flanked by the terminal noncoding sequences of genomic RNA segment 8, which encodes the [r] ...
7
Vaccinia virus RNA helicase: nucleic acid specificity in duplex unwinding.
... present study of the sub- strate requirements for duplex unwinding, together with earlier work, provides a working model for helicase ...the duplex segment to be ...
5
In Vitro Selection of Functional Nucleic Acid Sequences
... Synthesis of Oligonucleotide Libraries The starting point for SELEX experiments, whether the library is to be composed of ssDNA, dsDNA, or RNA, is the generation of a pool of oligodeoxynucleotides with a standard ...
9
Nucleotide sequences important for translation initiation of enterovirus RNA.
... An analysis by Kuge and Nomoto (20) of the replication process of in vitro mutants has suggested that the nucleotide sequence from positions 564 to 599 in the 5' noncoding sequence of th[r] ...
7
Essential Role of Cyclization Sequences in Flavivirus RNA Replication
... nucleotide sequences per ...Flavivirus RNA replication will be possible when efficient systems are established for in vitro assays of Flavivirus RNA synthesis using specific RNA ...
10
RNAcentral: a hub of information for non-coding RNA sequences.
... or RNA type of interest are downloaded and then the novel ncRNAs are compared against the RNAcen- tral sequences to classify them or to determine if the ncR- NAs have been observed ...to study miRNA ...
9
RNAcentral : a hub of information for non-coding RNA sequences
... or RNA type of interest are downloaded and then the novel ncRNAs are compared against the RNAcen- tral sequences to classify them or to determine if the ncR- NAs have been observed ...to study miRNA ...
9
In Vitro Selection of RNA Binding Peptides
... In vitro peptide and protein selection using mRNA display* enables the discovery and directed evolution of new molecules from combinatorial ...in vitro selection of peptide and protein libraries of more ...
199
Binding specificities of human RNA-binding proteins toward structured and linear RNA sequences
... Analysis of the biological roles of the RBP motif matches further indicated that many motif matches were conserved and specifically located at genomic fea- tures such as splice junctions. In particu- lar, our analysis ...
13
Sequences at the 3′ Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase
... Ba3⬘⫹34 and Ba3⬘noA, were used to synthesize the DNA fragments for direct transcription to produce the RNAs r138/40A, r138/noA, r84/noA, rABC, r34/ 40A, and r34/noA, respectively. Transcript r34/20A was derived from ...
7
Analytical study of avian reticuloendotheliosis virus dimeric RNA generated in vivo and in vitro.
... Results obtained with Rous sarcoma virus, Moloney murine leukemia virus (MoMuLV), and human immunodeficiency virus type 1 (HIV-1) show that the DLS maps within a domain necessary for vir[r] ...
8
Alfalfa Mosaic Virus Coat Protein Bridges Rna and Rna-dependent Rna Polymerase in Vitro
... AMV RNA-dependent RNA polymerase recognizes the 3' terminus of the viral RNA for precise initiation of viral RNA ...in vitro, the AMV coat protein mediates indirect interactions between ...
25
RNA Sequences Involved in Transcriptional Termination of Respiratory Syncytial Virus
... this study, we used dicistronic RNA replicons of RS virus, which directed viral transcription when coexpressed in cells with the N, P, L, and M2-1 proteins by means of a vaccinia virus-T7 expression system ...
9