RNAi/siRNA
Targeted siRNA Delivery Methods for RNAi-Based Therapies
... the RNAi effector (siRNA) that was fully complementary with the normal B-RAF mRNA and transiently transfected rather than stably incorporated into target ...of siRNA offer the benefit of repeated ...
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RNAi mechanisms in Huntington’s disease therapy: siRNA versus shRNA
... patients. RNAi is a potentially curative therapy for ...employs siRNA or antisense oligonucleotides (ASO) to knock down mutant Huntingtin mRNA ...than siRNA when tested head-to- ...compare ...
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Decoupling the downstream effects of germline nuclear RNAi reveals that H3K9me3 is dispensable for heritable RNAi and the maintenance of endogenous siRNA-mediated transcriptional silencing in Caenorhabditis elegans
... dsRNA-triggered RNAi does not signifi- cantly change the overall Pol II level at the target gene, but causes a shift of the Pol II profile instead (a reduction at the 3 ′ end and a gain in the gene ...
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Confirming the RNAi mediated mechanism of action of siRNA based cancer therapeutics in mice
... modified siRNA targeting the essential cell-cycle proteins polo-like kinase 1 (PLK1) and kinesin spindle protein (KSP) in ...mice. siRNA formulated in stable nucleic acid lipid par- ticles (SNALP) displayed ...
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siRNA Targeting of Thymidylate Synthase, Thymidine Kinase 1 and Thymidine Kinase 2 as an Anticancer Therapy: A Combinatorial RNAi Approach
... with RNAi substrates from an exogenous source may be a method to optimize dosage or concentration, such that a balance is achieved between exogenous antisense (and on-target effects) and endogenous miRNAs (to ...
183
RNA Interference: An Evolutionary Pathway in Periodontal Tissue Engineering
... apoptosis, RNAi may favour tissue regeneration. Cathepsin B–targeted siRNA also prevents chondrocytes from dedifferentiation, provid- ing a potential tool to prevent cartilage ...factor-α-targeted ...
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Role of RNA Interference (RNAi) in Dengue Virus Replication and Identification of NS4B as an RNAi Suppressor
... A consensus in silico model for the topology of NS4B suggested that it contains five trans-membrane domains, designated TMD1, -2, -3, -4, and -5. Furthermore, expression studies using plasmids containing different NS4B ...
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Anti-angiogenic efficacy of 5′-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs
... with RNAi-mediated silencing of VEGF in one RNA molecule, we designed a siRNA targeting both human and murine VEGF, and generated a 5′-triphosphate-VEGF-specific siRNA (ppp-VEGF) by in vitro ...
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Original Article Mechanism of SKOV-3 inducing hMSC homing
... LV3-CXCR4 siRNA and LV3-CBLL1 RNAi, and hMSC, human ovarian cancer cell SKOV-3, as well as human cervical cancer Hela cells, to investigate the mechanism of hMSC homing induced by ...
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Defense Mechanisms against Viral Infection in Drosophila: RNAi versus Non-RNAi
... the RNAi machinery than ...the siRNA pathway factors Dcr-2 and Ago-2 also have a role in the nucleus to control the processivity of RNA polymerase II on euchromatic loci ...the siRNA pathway in ...
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Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes
... (final conc. 25 nM) and observed the cells using a con- focal laser scanning microscope (CLSM) following 24 and 48 h of incubation (Figures 8A and 8B). In the case of RNAiMAX, green fluorescence was rarely observed in ...
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Plant Resistance to Root-Knot Nematodes through RNA Interference Targeting a Parasitism Gene.
... similar RNAi pathways to ...for RNAi in ...on RNAi soaking of plant parasitic nematodes, including Meloidogyne incognita, (Urwin et ...using RNAi to alter host-parasite ...using RNAi is ...
153
Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana
... hpRNA-induced RNAi uses 21–23 bp siRNA fragments to degrade target transcripts, we developed a hypothesis that expression of a single hpRNA fragment consisting of different gene specific tags (GSTs) in ...
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Sequence Homology Required by Human Immunodeficiency Virus Type 1 To Escape from Short Interfering RNAs
... of RNAi is sequence specificity, the degree of homology between the siRNA and the target sequence is ...the siRNA duplex prevented target RNA cleavage ...to siRNA (46), even when the mismatch ...
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RNAi therapy to the wall of arteries and veins: anatomical, physiologic, and pharmacological considerations
... of RNAi therapy adjuncts can be significant and have to be carefully ...of RNAi delivery to the vascular wall, it remains to be seen if a single target can be sufficient to the achieve clinically desirable ...
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RNAi THERAPEUTICS
... of siRNA directs the endonuclease activity of the RISC to the homologous (target) site on the m-RNA resulting m-RNA cleavage 19 ...RISC siRNA is initially duplexed but unwinds in the activated mRNA cleavage ...
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Development of Non-Covalent Functionalization of Carbon Nanotubes for siRNA Delivery
... on RNAi therapy have been actively carried out [38, ...function, RNAi is ...on RNAi therapy is using siRNA “ALN-PCS” which inhibits PCSK9 synthesis and in turn reduces the blood concentration ...
203
Protein-based vehicles for biomimetic RNAi delivery
... cholesterol-conjugated siRNA and antisense oligonucle- otides ...philic siRNA conjugates and their relative efficacy in murine hepatic delivery ...therefore siRNA efficacy, of ApoE-rHDL over ...to ...
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The RNAi Inheritance Machinery of Caenorhabditis elegans
... nuclear RNAi to maintain germline immortality and the gene targets of HRDE-1 are known (Buckley et ...an siRNA-directed H3K9 methyltransferase and that siRNA-directed H3K9 methylation is an obligate ...
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Photoperiodic diapause under the control of circadian clock genes in an insect
... Background: Most organisms have evolved a circadian clock in order to anticipate daily environmental changes and many of these organisms are also capable of sophisticated measurement of daylength (photoperiodism) that is ...
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