RPMI 1640
Interlaboratory evaluation of Etest method for testing antifungal susceptibilities of pathogenic yeasts to five antifungal agents by using Casitone agar and solidified RPMI 1640 medium with 2% glucose
... solidified RPMI 1640 medium with 2% glucose and Casitone ...with RPMI 1640 medium when testing amphotericin B (84 to 90% versus 1 to 4%), itraconazole (87% versus 63 to 74%), and ketoconazole ...
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Evaluation of the Anti-cancer Activity of most potent Ethanolic fraction of Prunus avium on EAC Cells in RPMI 1640
... on RPMI 1640 medium: Number of viable and non- viable cells found under microscope was counted on 2, 5 and 8 hours (n=10) for each concentration and Percentage Inhibition values were calculated by using ...
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Modulation of Morphogenesis in Candida albicans by Various Small Molecules
... Various inhibitors of actin dynamics, including latrunculin, jas- plakinolide, and cytochalasin A, also blocked the Y-H transi- tion induced in serum-containing, Spider, or M199 medium at 37°C (1, 50, 135, 147). ...
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DEVELOPMENT OF HERBAL FORMULATION WITH AJWA SEED (PHOENIX DACTYLIFERA L ) EXTRACT AND ZAM ZAM WATER FOR INVITRO ANTICANCER ACTIVITY
... in RPMI-1640 medium supplemented with FBS (fetal bovine serum), 100units/ml penicillin, 100μg/ml streptomycin, and were cultured in a humidified atmosphere of 5% CO2 and 95% air at ...in ...
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Attempted Isolation of Nanobacterium sp Microorganisms from Upper Urinary Tract Stones
... Cell coculture. Gimenez staining of inoculated 3T6 cells and on inoculated RPMI 1640 medium failed to reveal nanobacte- ria after 1 week of culture in any of the 10 stone samples (Table 1). However, after 3 ...
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VER-246608, a novel pan-isoform ATP competitive inhibitor of pyruvate dehydrogenase kinase, disrupts Warburg metabolism and induces context-dependent cytostasis in cancer cells
... The following day the cells were treated with the indicated concentrations of VER-246608 and Nov3r in RPMI-1640 media containing 0.25 g/L D-glucose and 2% dialyzed FCS, followed by the r[r] ...
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Limited response of NK92 cells to Plasmodium falciparum infected erythrocytes
... As controls, pure RPMI-1640 and supernatants of NK92 cells cultured in their normal growth medium + rIL-2 and under a 24 hours period of “starvation” cell medium without rIL-2 as well as[r] ...
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GPR101 mediates the pro resolving actions of RvD5n 3 DPA in arthritis and infections
... on human macrophages. (A) Human monocyte–derived macrophages were incubated with either an siRNA against GPR101 or a control sequence (CT siRNA) for 96 hours, and GPR101 expression was assessed using flow cytometry (n = ...
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Production, expansion and clonal analysis of T cells with specific HLA restricted male lysis.
... Oxnard, Cahf) kept at an angle of 45° in a humidified 5% CO 2 incubator The culture medrum was RPMI-1640 that was supplemented with 20 raM L-glutamine/ml (Flow Laboratories Lid , Irvine[r] ...
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Nano-Se crosstalks with nano-DOX/FU to selectively hack hepatic cancer cells and spare normal cells healthy: A mechanism-based study
... All drug groups were evaluated by MTT assay using HepG2/BHk-21 cells. Briefly, the cells were cultured in 96-well plates at a density of 5×10 3 cells/well. All drugs with their described concentrations were added in ...
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Plasmodium vivax: paroxysm associated lipids mediate leukocyte aggregation
... of RPMI 1640 [1] in a 24-well tissue culture plate (Flow Lab- oratories, UK) and 200 μ l of test (paroxysm plasma, pre- paroxysm plasma, post-paroxysm plasma, non-malarial fever plasma or plasma from ...
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Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT 8 cells
... of RPMI 1640 with L -glutamine (Sigma R-5382, lot 33H46291), 15 mM N-2-hydroxyethylpiperazine-N 9 -2-ethanesulfonic acid (HEPES) buffer, 100 U of penicillin per ml, 100 m g of streptomycin per ml, and ...
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Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
... USA). RPMI-1640 culture medium, fetal bovine serum (FBS), L-glutamine, and penicillin– streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, ...
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Cell mediated Immunity in Legionnaires' Disease
... Freshly explanted monocytes from a nonpatient were incubated in monolayer culture for 24 h at 37°C in 5% C02-95% air in 500 IAI RPMI 1640 medium containing 20% fresh nonpatient serum and[r] ...
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Axitinib Targeted Cancer Stemlike Cells to Enhance Efficacy of Chemotherapeutic Drugs via Inhibiting the Drug Transport Function of ABCG2
... diphenyllapatinibrazolium bromide (MTT), vincristine, topotecan, mitoxantrone, Dox, rhodamine 123, verapamil, MK571 and FTC were products of Sigma Chemical Co (St. Louis, MO, USA). Axitinib was pur- chased from Selleck ...
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Binding, uptake, and intracellular trafficking of phosphorothioate modified oligodeoxynucleotides
... In these studies, K562 cells 2 x 10' cells/ml suspended in RPMI 1640 with 2% bovine calf serum GIBCOBRL were incubated in the presence or absence of 100 nM c-myb antisense 'P-ODN at 4°C [r] ...
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Niclosamide enhances abiraterone treatment via inhibition of androgen receptor variants in castration resistant prostate cancer
... Cell growth assay C4-2B parental or C4-2B MDVR cells were seeded on 12-well plates at a density of 1×105 cells/well in RPMI 1640 media containing 10% FBS and treated with varying concent[r] ...
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High Glucose Induces Reactivation of Latent Kaposi's Sarcoma-Associated Herpesvirus
... To further confirm that high glucose levels induce H2O2 production, we cultured BCBL1-BAC36 cells in RPMI 1640 medium containing 1, 3, November 2016 Volume 90 Number 21 and 6 g/liter D-g[r] ...
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Prevalence, clinical relevance and characterization of circulating cytotoxic CD4+CD28 T cells in ankylosing spondylitis
... ment, fresh PBMCs 1 × 105 from three HLA-B27 positive AS patients were incubated in RPMI 1640 and 10% fetal calf serum together with the Tap 1 and 2 and MHC class II deficient HLA-B*2705[r] ...
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Transcriptional Profiling Identifies a Role for BrlA in the Response to Nitrogen Depletion and for StuA in the Regulation of Secondary Metabolite Clusters in Aspergillus fumigatus
... to RPMI 1640 medium (Sigma-Aldrich product number R6504) buffered at pH ...MOPS-buffered RPMI 1640 medium, an aliquot of each strain was removed and RNA was extracted with the Qiagen RNeasy ...
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