RT-PCR, reverse transcription PCR.
Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... However, it should be noted that dPCR did not overcome all sequence mismatch- induced amplification inefficiencies, as evidenced by genotype sequence group N (Fig. 2), which has a single mismatch near the middle of the ...
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Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice
... interleukin; RT-PCR: reverse transcription PCR; DAVID: database for annotation, visualization and integrated discovery; DEGs: differentially expressed genes; GO: gene ontology; IBD: ...
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Transfer and Evaluation of an Automated, Low Cost Real Time Reverse Transcription PCR Test for Diagnosis and Monitoring of Human Immunodeficiency Virus Type 1 Infection in a West African Resource Limited Setting
... real-time reverse transcription- PCR (RT-PCR) assay was transferred to the laboratory of the Centre de Diagnostic et de Recherches sur le SIDA, Abidjan, Co ˆte d’Ivoire, and assessed ...
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Comparison of Commercial Real Time Reverse Transcription PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
... Active PRRSV surveillance in boar studs relies mainly on col- lection and testing of serum, semen, blood swabs, and, more re- cently, oral fluids (6, 7). The choice of sample type should take into consideration the ...
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Development of Multiplex PCR Ligase Detection Reaction Assay for Detection of West Nile Virus
... multiplex reverse transcription-PCR ligase detection reaction (RT-PCR/LDR) assay for the detection of West Nile virus (WNV) in both clinical and mosquito pool ...the PCR is ...
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Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... Other RT-PCR assays have used two primer sets specific for the H1N1 and H3N2 hemagglutinin genes to detect influenza A virus subtypes (24) or three primer sets to distinguish influenza A virus subtypes and ...
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... one-step reverse transcription-PCR (RT-PCR) assay specific for the detec- tion of influenza A H5 viruses from clades 1, 1 ⬘ , 2, and 3, together with the inclusion of a distinguishable ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... the PCR-ELISA system from Boehringer ...of PCR product, and the product was denatured by adding 25 ml of ...as PCR positive or negative according to a cutoff OD value of ...of PCR products ...
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Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one tube reverse transcription PCR assay
... pharmacy). RT-PCR was performed on a programmable thermocycler (MJR model PTC- 100-60; Fisher Scientific) as follows: 42 8 C for 30 min (cDNA synthesis), 95 8 C for 3 min (RT enzyme inactivation and ...
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A one tube method of reverse transcription PCR to efficiently amplify a 3 kilobase region from the RNA polymerase gene to the poly(A) tail of small round structured viruses (Norwalk like viruses)
... The RT-PCR products obtained from the RNA polymerase region are small (100 to 500 bp) (3, 11, 12, 14, 20, 31, 33, 44), whereas the distance from this region to the poly(A) tail is about 3 kb and includes ...
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Preclinical Evaluation of Two Real Time, Reverse Transcription PCR Assays for Detection of the Severe Acute Respiratory Syndrome Coronavirus
... We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR RT-PCR assay targeting the open reading frame ORF 1b region of the se[r] ...
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Performance and Cost Evaluation of One Commercial and Six In House Conventional and Real Time Reverse Transcription PCR Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus
... in-house reverse transcription-PCR (RT-PCR) as- says targeting several areas of the viral genome have been described (2, 4, 7, 9, ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... There is currently a global program attempting to eliminate lymphatic filariasis (LF) by administering drugs to affected communities with the goal of interrupting transmission of the parasite. At present the c ...
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Cloning of a muscle specific calpain from the American lobster Homarus americanus: expression associated with muscle atrophy and restoration during moulting
... Here we report the cloning of a crustacean calpain gene with features that distinguish it from calpains isolated from other species. The cDNA encodes a non-EF-hand calpain with a unique N-terminal sequence and an ...
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Hepatitis D virus RNA editing: specific modification of adenosine in the antigenomic RNA.
... subsequent transcription of the antigenomic RNA would yield cytidine at position 1012 in the genomic RNA, which would then be a template for the production of full-length antigenomic RNA with 1012G, as well as the ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... by reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the rapid ...
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Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... TaqMan RT-PCR detected DOBV RNA in 7 of 474 rodent tissue ...nested PCR yielded a 389-bp band for 2 of 474 rodent tissue ...TaqMan RT-PCR (Ta- ble ...nested PCR by use of the ...
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Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay
... usual PCR, after that, ELISA is performed to receive desired ...time. PCR-ELISA does not require using dangerous and carcinogenic material that have used in many of tech- niques, including of methylene blue ...
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Epidemiological and Genetic Overview of the Klebsiella pneumoniae Carbapenemases (KPCs) in K. pneumoniae Isolated from the Clinical Samples in Iran
... A reaction mixture, consisting of 5 µg of RNA, was incubated in DEPC-treated water and 1 µL of random hexamers (Pars Tous, Iran). Then, it was incubated for five minutes at 65 ºC and cooled down on the ice. After 10 µL ...
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Understanding protoplast technology as a tool to enhance biocontrol
... (bisulphite PCR), but not through multiple replicates as seen in this project, which suggests that the individual 5mCs had a higher proportion of methylation than what has occurred ...
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