RT, reverse transcriptase
Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... (Sharkey et al. 1994; Birch et al. 1996, Hermann and Patel 2000; Gening et al . 2006; Kermekchiev et al. 2003). The activation is obtained by heating up to ≥95 °C during the initial denaturation step of the PCR. In ...
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Decreased Processivity of Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) Containing Didanosine-Selected Mutation Leu74Val: a Comparative Analysis of RT Variants Leu74Val and Lamivudine-Selected Met184Val
... the reverse transcriptase (RT) of human immunodeficiency virus 1 (HIV-1) are converted to the triphosphates, and the nucleoside triphosphates (dNTPs) are incorporated into elongating DNA to greatly ...
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Two Coselected Distal Mutations in HIV-1 Reverse Transcriptase (RT) Alter Susceptibility to Nonnucleoside RT Inhibitors and Nucleoside Analogs
... Any RT that disassociates from the labeled T/P is more likely to bind to the cold trap, which is present in ...the RT is limited to one cycle of primer ...the RT remains bound to the labeled ...
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Nonnucleoside Reverse Transcriptase Inhibitor-Resistant HIV Is Stimulated by Efavirenz during Early Stages of Infection
... of RT in ...increase RT content and virus ...recombinant RT protein in a His-tagged p51 (His-p51) pull- down ...mutant RT subunits in ...mutant RT (G190S, G190S ⫹ K101E [ES], and L74V ⫹ ...
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Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity.
... activity without measurably affecting polymerase activity (2, 14). The X-ray crystal structures of HIV-1 RT heterodimers show that C280 lies near the middle of a helix I in the thumb subdomains of both p66 and ...
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HIV 1 associated PKA acts as a cofactor for genome reverse transcription
... p66 RT subunits and intrinsic RT activ- ity was unaffected as evidenced in exogenous RT assays performed using a synthetic poly(rA)/oligodT ...to reverse transcribe the viral genome into FL ...
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Prevalence of reverse transcriptase and protease mutations associated with antiretroviral drug resistance among drug-naïve HIV-1 infected pregnant women in Kagera and Kilimanjaro regions, Tanzania
... Only one primary mutation associated with NRTI resist- ance, V118I, which causes low-level resistance to Lamivu- dine (3TC) and possibly other NRTIs when present in combination with E44E/D, was observed in three strains ...
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COMPARING THE GENES OF Artemisia annua AND Artemisia ludoviciana WITH RESPECT TO THE ARTEMISININ BIOSYNTHETIC PATHWAY
... the reverse transcriptase polymerase chain reactions (RT-PCR) done on RNA extracts obtained from the fresh leaves of Artemisia ludoviciana with the Artemisia annua primers are summarized in table ...
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INSILICO SCREENING OF NNRTI AS POTENTIAL TARGETS FOR TUMOR CONTROL
... Non-nucleoside reverse transcriptase inhibitors (NNRTIs) were identified as a new class of antiretroviral drugs over the past twenty years by means of dedicated research and development towards HIV ...
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A Non-Long Terminal Repeat Retrotransposon Family Is Restricted to the Germ Line Micronucleus of the Ciliated Protozoan Tetrahymena thermophila
... a reverse transcriptase (RT) (reviewed in reference ...target-primed reverse transcription, re- verse transcribing a cDNA copy of their RNA directly into the nicked chromosomal target site ...
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Detection and identification of aquatic birnaviruses by PCR assay
... A reverse transcriptase polymerase chain reaction (RT-PCR) assay was developed for the detection and identification of aquatic ...the RT-PCR assay was evaluated by comparison of the results ...
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METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF EMTRICITABINE, TENOFOVIR AF, DOLUTEGRAVIR IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
... inhibiting reverse transcriptase, the enzyme that copies HIV RNA into new viral ...HIV-1 reverse transcriptase (RT) both by competing with the natural substrate deoxycytidine ...
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Sensitive reverse transcriptase assay to detect and quantitate human immunodeficiency virus
... A sensitive biochemical assay of viral reverse transcriptase RT was developed that is useful for both the detection and quantitation of human immunodeficiency virus HIV, the agent respon[r] ...
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Fully spliced HIV 1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT treated cells
... RNA Reverse transcription of FL RNA is inhibited in the pres- ence of AZT, an inhibitor of the reverse transcriptase (RT) widely used in the clinic for the treatment of HIV infec- ...inhibits ...
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Eye Findings in Infants With Suspected or Confirmed Antenatal Zika Virus Exposure
... with reverse transcriptase polymerase chain reaction (RT-PCR)– positive and unconfirmed case patients who were born during and soon after an outbreak period is used to justify the diagnosis of ZIKV ...
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Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... samples. Reverse hybridization yielded clear VP4 genotypes for approximately 98% of the PCR-positive samples, but all samples were positive for the VP4 universal ...both RT-PCR and reverse ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... Background: The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction ...
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Differential Sensitivities of Retroviruses to Integrase Strand Transfer Inhibitors
... Nonnucleoside reverse transcriptase (RT) inhibitors such as nevirapine are highly selective for HIV-1 (50), whereas the nucleoside RT inhibitor (NRTI) azidothymidine (AZT) inhibits infection ...
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Characterization of Hepatitis B Viral Drug Resistance in Indian Subcontinent Patients with Chronic Liver Disease.
... polymerase, reverse transcriptase (rt) and other accessory ...HBV reverse transcriptase (HBVrt) gene contains several regions that are homologous to other RNA- dependent polymerases and ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... using RT-qPCR entails the synthesis of cDNA by the reverse transcriptase (RT) enzyme, followed by an exponential amplification of the template by DNA polymerase, any chemical moiety, which is ...
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