S. flexneri
Genome sequence of Shigella flexneri strain SP1, a diarrheal isolate that encodes an extended-spectrum β-lactamase (ESBL)
... Shigella flexneri remains a public health concern throughout the world and its pathogenesis should be further ...Shigella flexneri (ipaD), serine pro- tease autotransporters of Enterobacteriaceae (pic), ...
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Shigella Flexneri Ghost Cells as a Vaccine with Correct Surface Antigens
... randomly divided into two groups (15 rats/each), intraperitoneally vaccinated group with SFGs (SFG group) at 70μg in 100μl PBS with 100μl complete Freund‘s adjuvant and the second group is control group (CG) received ...
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Shigella Outer Membrane Protein PSSP-1 Is Broadly Protective against Shigella Infection
... Cloning and purification of PSSP-1. A PCR-amplified fragment car- rying PSSP-1 (IcsP amino acids 171 to 300; GenBank accession no. AAP78966.1) (17) was cloned between the EcoRI and XhoI sites of pET21-d and pET24-d ...
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Transcriptional adaptation of shigella flexneri during infection of macrophages and epithelial cells: insights into the strategies of a cytosolic bacterial pathogen
... of S. flexneri metabolism during infection, and our intracellular gene expression data offer the first insight into this ...(29), S. flexneri is unable to utilize two-carbon compounds via the ...
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Comparing Invasive and Non-Invasive of Isolated Shigella flexneri by Electron Microscopy of Cell Culture, SDS-PAGE and Congo Red Method
... of S. flexneri into the HeLa cell culture, demonstrated by EM and also, this band protein was found in all ...S. flexneri strains with Congo red dye ...of S. flexneri to enter ...
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Temperature-regulated expression of outer membrane proteins in Shigella flexneri
... isolate SH057 expressed at 37, 38.5, and 40°C were loaded in each well and profiled using PROTEAN II xi (Bio-Rad). The OMPs obtained were resuspended with sample buffer containing 0.1% SDS and 2-β-mercap- toethanol ...
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Quality of commercially produced Shigella serogrouping and serotyping antisera
... 1 antiserum gave 4+ reactions with all serotype 1 strains tested, but the other Roach serotyping antisera were negative with 17 of 26 homologous strains of S.. flexneri antisera gave str[r] ...
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Evaluation of Molecular Methods for Serotyping Shigella flexneri
... Phylogenetic analysis of the whole-genome sequences of 124 isolates indicated that the serotype, regardless of whether it was phenotypically or genotypically determined, was a weak predictor of phylogenetic relationships ...
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Epidemic characterization and molecular genotyping of Shigella flexneri isolated from calves with diarrhea in Northwest China
... China. S. flexneri 2a was first isolated from a yak with diarrhea in Tibet in ...among S. flexneri isolates and tested for antimicrobial susceptibil- ity ...
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Linalool, citral, eugenol and thymol: control of planktonic and sessile cells of Shigella flexneri
... Therefore, the naturally occurring phytochemicals studied in this paper, specially citral and timol isolated or combined could be used for microbiological control in the food industry substituting chemical compounds. The ...
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Escape of Actively Secreting Shigella flexneri from ATG8/LC3 Positive Vacuoles Formed during Cell To Cell Spread Is Facilitated by IcsB and VirA
... and S. flexneri, and the en- suing recruitment of LC3 (11, 27, ...WT S. flexneri, FIG 5 Scheme describing the role of the effectors IcsB and VirA in the escape of actively secreting ...
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Shigella flexneri Regulation of ARF6 Activation during Bacterial Entry via an IpgD Mediated Positive Feedback Loop
... at S. flexneri entry ...for S. flexneri entry and for ARF6 recruitment to entering bacteria by treating cells with SecinH3, a small-molecule cytohesin family-specific inhibitor (42) that ...
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Shigella flexneri Adherence Factor Expression in In Vivo-Like Conditions
... The mutational and complementation analyses demonstrated functional adherence roles for the products of the lpfA, fimA, and csgAB structural genes. While additional direct evidence of each structure is required and such ...
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Multi Drug Resistant Shigella flexneri – An Emerging Threat to Community
... healthcare facilities to the public. Though the patient was treated successfully, but spread of such clones may pose a greater threat to the community and make the situation bleaker with limited therapeutic options as ...
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Characterization of endemic Shigella flexneri strains in Somalia: antimicrobial resistance, plasmid profiles, and serotype correlation
... Characterization of Endemic Shigella flexneri Strains in Somalia: Antimicrobial Resistance, Plasmid Profiles, and Serotype Correlation MARIASSUNTA CASALINO,'* MAURO NICOLETTI,2 ANNAMARIA[r] ...
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Sensitive receptor specified enzyme linked immunosorbent assay for Escherichia coli verocytotoxin
... It is of interest that the strains of Shigella sonnei and Shigella flexneri used in this study were negative in both the RELISA and the cytotoxicity assay Table 2, but other investigator[r] ...
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Preparation, characterization and bioactivity evaluation of Co(II), Ni(II) and Cu(II) complexes containing phthalylsulphathiazole and benzoate ligands
... confirm the entry of the ligands into the coordination sphere. The electronic spectral data indicate the probable geometry of the complexes. The antimicrobial and anti fungal screening of the complexes against the ...
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American Journal of Infectious Diseases and Microbiology
... was significant (p<0.0001). The bacterial species most represented in the stool samples were: Shigella flexneri (1.16%), Vibrio cholerae (1.14%), Salmonella sp. (0.74%), Escherichia coli (0.62%), Campylobacter ...
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Cardiolipin Synthesis and Outer Membrane Localization Are Required for Shigella flexneri Virulence
... Cardiolipin synthesis is required for plaque formation. To determine the role of cardiolipin in S. flexneri pathogenesis, we performed plaque assays (47) with each of the cardiolipin synthesis mutants. Plaque ...
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Statistical Analysis of Strength Property of Soil
... Shigella flexneri, Kosakonia sacchari and Enterobacter species, Klebsiella vericola, Escherichia vulneris, and Escherichia coli strains on the basis of 16s rRNA sequences Submission to NCBI Gene ...
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