S/sup 2/I cells
Quantum dots modulate intracellular Ca<sup>2+</sup> level in lung epithelial cells
... many cells that strongly responded to the QD deposition (Figure ...10 s, matching the response time of the TEER previously shown in Figure ...[Ca 2+ ...
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Novel intravaginal nanomedicine for the targeted delivery of saquinavir to CD4<sup>+</sup> immune cells
... In brief, SQV was dissolved in methanol, making a 10 mg/mL SQV stock solution. SQV (50 µ L) was combined with 30 mg/mL of PLGA–carboxylic acid ethyl acetate solution (575 µ L), forming the organic phase. The organic ...
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Changes in the number of CD31<sup>−</sup>CD45<sup>−</sup>Sca-1<sup>+</sup> cells and Shh signaling pathway involvement in the lungs of mice with emphysema and relevant effects of acute adenovirus infection
... of 2 × sodium dodecyl sulfate (SDS) loading buffer ...6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue, 5% 2-mercaptoethanol) for 10 min at 95 ° ...for 2 h at 25 ° ...
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Effect of pedicle fixation combined with <sup>125</sup>I seed implantation for metastatic thoracolumbar tumors
... tumor cells, stabilizing spinal ...root; 2) there is a pathological vertebral fracture caused by tumor damage, and there is existing or predicted spinal cord instability; 3) there is intractable pain, and ...
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Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles
... manufacturer’s guidelines. Purified HPCs (95% ± 3% [n = 3]) were positive for CD34. HPCs were cultured for 12 days in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco, Paisley, UK) supplemented with 10% fetal bovine ...
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Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway
... endothelial cells Western blotting analysis Diabetic mouse aortic and rat aortic endothelial cells were homogenized in ...for 2 h at room tem- perature. Membranes were incubated with 2 μg/mL ...
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Defective eosinophil chemotaxis to eotaxin in a patient with chronic lower baseline CD4<sup>+ </sup>T-lymphocytes and elevated CD8<sup>+</sup> T cells
... Results: The patient’s eosinophils had defective chemotaxis, shape changes, and F-actin reor- ganization against eotaxin, when compared to the controls. CCR3 surface and total expression was highest in allergic subjects ...
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The role and significance of VEGFR2<sup>+</sup> regulatory T cells in tumor immunity
... Recently, there has been an emergence of many targeted drugs, like EGFR inhibitor, tyrosine kinase inhibitor (TKI), monoclonal antibodies, and so on. Many TKIs like sunitinib and sorafenib can inhibit tumor growth via ...
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PD-1 blockade restores impaired function of ex vivo expanded CD8<sup>+</sup> T cells and enhances apoptosis in mismatch repair deficient EpCAM<sup>+</sup>PD-L1<sup>+</sup> cancer cells
... cytotoxicity in colorectal cancer cells in the presence of a PD-1 mAb. We observed an insignificant apoptotic effect in MMR proficient TECs in the presence of PD-1 mAb after 48 hrs (Figure S1C). Therefore, MMR ...
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CD44<sup>+</sup>/CD24<sup>-</sup> breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold
... MDA-MB-435S cells exhibit various 3D morphologies in response to different 3D culture scaffolds, which were identified by light micro- scopy and localization of F-actin in colonies at the endpoint of 5 days ...
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Blockage of store-operated Ca<sup>2+</sup> entry antagonizes Epstein–Barr virus-promoted angiogenesis by inhibiting Ca<sup>2+</sup> signaling-regulated VEGF production in nasopharyngeal carcinoma
... HK1 cells with the infectious rEBV, which served as a visible indicator for the EBV-positive ...EBV-positive cells that were captured after the co-cultivated EBV ...of cells were selected by ...
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Alteration of gene expression profile in CD3<sup>+</sup> T-cells after downregulating MALT1
... > 2-fold: 17 genes; > 4-fold: 1 gene) were upregulated and 854 genes ( > 2-fold: 104 genes, > 4-fold: 1 gene) were downregulated at least ...
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Aberrant histone modification in CD19<sup>+</sup> B cells of the patients with chronic lymphocytic leukemia
... It was shown that the expression level of SIRT1 and EZH2 mRNA in patients with CLL was significantly higher than that of healthy controls (P = 0.03 and P = 0.02, respec- tively), as shown in Figures 2A and 3A. We also ...
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Mechanotransduction is required for establishing and maintaining mature inner hair cells and regulating efferent innervation
... Fig. 4 IHCs from Myo7a fl/fl Myo15-cre +/- conditional knockout mice acquire their mature phenotype. a, b MET currents from IHCs of control Myo7a fl/fl (left, P10) and knockout Myo7a fl/fl Myo15-cre + /- (right, P9) mice. The ...
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<p>Isovitexin Suppresses Cancer Stemness Property And Induces Apoptosis Of Osteosarcoma Cells By Disruption Of The DNMT1/miR-34a/Bcl-2 Axis</p>
... Emerging evidence has indicated that epigenetic regula- tion acts as a driver of tumorigenesis by regulating stem-like features of CSLCs. 6,7 Aberrant DNA methylation can pro- mote progression of OS. 8,9 DNA ...
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Bisphenol A promotes cholesterol absorption in Caco-2 cells by up-regulation of NPC1L1 expression
... In sup- porting this hypothesis, when Caco-2 cells were stimu- lated with BPA, we did find significantly increased expression of the SREBP-2 in the cells, and when SREBP-2 was ...
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Frequent infiltration of S-100 protein<sup>+</sup> CCR5<sup>+</sup> immature dendritic cells in damaged bile ducts of primary biliary cirrhosis compared to cholangiocellular carcinoma
... Figure 2 Immunohistochemistry and in situ hybridization demonstrating chemokine expression in primary biliary cirrhosis (stage ...epithelial cells of the bile ...epithelial cells of the bile ...
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Downregulation of CD44 reduces doxorubicin resistance of CD44<sup>+</sup>CD24<sup>-</sup> breast cancer cells
... stem cells were plated at a concentration of 1000 cells/mL in serum-free DMEM-F12, supplemented with 10 ng/mL basic fibroblast growth factor (bFGF), 20 ng/mL epidermal growth factor (EGF), 5 ng/mL insulin, ...
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Baicalin suppresses proliferation, migration, and invasion in human glioblastoma cells via Ca<sup>2+</sup>-dependent pathway
... U251 cells were treated with 0, 25, 50, 100, 200, and 300 µ M baicalin for 48 hours and then ...for 2 hours at room temperature, the blots were incubated at 4 ° C over- night with separate primary ...
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<em>N</em>-n-butyl haloperidol iodide inhibits H<sub>2</sub>O<sub>2</sub>- induced Na<sup>+</sup>/Ca<sup>2+</sup>-exchanger activation via the Na<sup>+</sup>/H<sup>+</sup> exchanger in rat ventricular myocytes
... (BCECF-AM, 2 µ M) in modified Krebs solution (135 mM NaCl, 5.9 mM KCl, 1.5 mM CaCl 2 , ...MgCl 2 , ...The cells were then washed three times and incubated for an additional 45 minutes in fresh ...
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