Sample collection and nucleic acid extraction
Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNA
... extract nucleic acid for use in amplification assays is the potential for cross-contamination of negative specimens as a consequence of aerosolization, faulty robotics, or robotic ...after extraction ...
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Real Time PCR for detection of herpes simplex virus without nucleic acid extraction
... Discussion The data described in this work indicate that real-time PCR for the detection of herpes simplex viruses can be per- formed without previous extraction and purification of nucleic acid from ...
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Comparative Evaluation of Three Commercial Systems for Nucleic Acid Extraction from Urine Specimens
... automated nucleic acid extraction plat- forms that can process a low number of clinical specimens are ...the extraction of BKV from urine specimens using real-time PCR, even though the miniMAG ...
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Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens
... QIAGEN extraction varied between ⫺ ...the nucleic acid ...easyMAG extraction, even when the specimen was stored for more than 1 year at ⫺ 70°C in the absence of a stabilizing lysis buffer, ...
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Nucleic Acid Electrophoresis Cell
... Convection is the most effective way to distribute heat evenly. The upper buffer dampens temperature fluctuations in the gel, and adds to the reproducibility of each run. The contact between the buffer and the gel plate ...
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Peptide Nucleic Acid Microarrays
... Peptide Nucleic Acid Microarrays Anette Jacob, Ole Brandt, Achim Stephan, and Jörg D. Hoheisel Summary A fast and economical procedure for the production of peptide nucleic acid (PNA) ...
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An Introduction to Peptide Nucleic Acid
... Furthermore, as the PNA probe is uncharged excess PNA probe will not migrate in an electrical field and the electrophoresis therefore offers a facile separation of bound and unbound PNA probe. This allows for elimination ...
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Quantitative and Qualitative Chemical Extraction of Deoxyribo Nucleic Acid DNA from Human Cell Organelles
... The non polar tail of SDS interact with non polar ends of the phospholipid to lyse the double layered membrane of cell organelles. The lysis make the enclosed DNA of cell organelles to come out in the buffer solution. ...
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
... Gel extraction was followed and modified from QIAquick Gel Extraction Kit (Qiagen ...ml collection tube in 650 μl increments, and centrifuged at ≥10,000 x g for 1 ...
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Search for a Prion-Specific Nucleic Acid
... 5A). After an initial decrease up to about 50 kJ/m 2 , the curves nearly level off. We observed two effects that might be responsible for the nonlinearity. First, the solutions exhibited a fairly high optical density, ...
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Polysaccharides as initiators of nucleic acid polymerization
... range 30 ˚C to 80 ˚C . Further temperature increase in the empirical sample resulted in the destruction of the poly- nucleotide. As can be seen from the above, PU demonstrated a significant catalytic activity in ...
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PNA Biosensors for Nucleic Acid Detection
... the sample handling and measurement steps, biosensors eliminate the need for the sample preparation and hence offer great promise for numerous on-site analytical applications (for which rapid, low-cost ...
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Rapid Development of Nucleic Acid Diagnostics
... background sample set also illustrates the reason why there have been many environmental false positives when using assays that have not been screened in this ...the sample with a single signature or ...the ...
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Development of Sorbents for Extraction and Stabilization of Nucleic Acids
... batch sample of target adsorbed on material was prepared. The sample was then divided into aliquots, and the supernatants were separated from the precipitated ...control sample consisting of the ...
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Detection of Methicillin Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real Time PCR
... Andrea J. Grisold, 1 * Eva Leitner, 1 Gerhard Mühlbauer, 2 Egon Marth, 1 and Harald H. Kessler 1 Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz, 1 and Roche Diagnostics GmbH, A-1211 Vienna, 2 Austria ...
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Setting up a NIPD Biobank of maternal samples: Sample collection, Storage and DNA extraction protocols
... Respect the fundamental rights of study individuals declared in the Charter of Fundamental Rights of the European Union and in the Directive 95/46/EC on the protection of individuals wit[r] ...
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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
... PNA FISH for the detection of BCR-ABL1 T315I mutation In addition, for the detection of T315I mutation in pro- genitor cells we set up a method based on fluorescently- labelled PNA probe (PNA FISH). CD34 + cells were ...
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Nucleic acid detection in the diagnosis and prevention of schistosomiasis
... DNA extraction and require new strategies to be ...contaminate nucleic acid amplification under unsuit- able laboratory conditions, thus giving rise to false- positive ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... [62] nucleic acid capture ...electrochemical nucleic acids probe made of PNA which exhibits high sensitivity and specificity when challenged with heterogeneous samples of ...
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