Scaffold proteomic software used for iTRAQ analysis
iTRAQ-based quantitative proteomic analysis provides insight for molecular mechanism of neuroticism
... proteome. iTRAQ was used to identified the characterize differentially expressed pro- teins between high and low neuroticism, which found that SNCA, ATP7A, GNG2, CDK6, MPO, AZU1, HIST1H2BH, ITGAM and MMP9 ...
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Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis
... Confirmation of heat stress-induced proteins in mouse sperm by western blotting To validate the proteomics finding, the proteins related to sperm motility and energy metabolism AKAP4, SPESP1, ODF1, ODF2, GAPDHS, and ...
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Potential tumor biomarkers identified in ovarian cyst fluid by quantitative proteomic analysis, iTRAQ
... Epithelial ovarian cancer consists of at least five different histological subtypes, and no known biomarker covers all histologies as a single marker [24]. To increase our chances of finding a true novel biomarker, we ...
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iTRAQ based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt
... we used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS methods to analyze changes in the proteome of the regenerating newt limb that occurred 2 h and 8 h after ...
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An iTRAQ-based proteomic analysis reveals dysregulation of neocortical synaptopodin in Lewy body dementias
... However, it is difficult to collect matched brain samples at various time points during the temporal evolution of the disorder. Prospective studies through neuroimaging or systems biological approaches involving CSF ...
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Quantitative proteomic analysis by iTRAQ® for the identification of candidate biomarkers in ovarian cancer serum
... DIGE analysis of immunode- pleted sera that levels of AACT and LRG1 expression are increased in ovarian cancer sera, and validated this by Western blot ...was used for the initial validation, as the ...
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iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress
... 2.3. Protein extraction Samples were initially frozen dried. The powder was resus- pended in 200 m l of dissolution buffer (0.5 M triethyl ammonium bicarbonate at pH 8.5). The protein disul fide bonds of the solution ...
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ITRAQ-based quantitative proteomic analysis of processed Euphorbia lathyris L. for reducing the intestinal toxicity
... study used iTRAQ labeling followed by 2D-LC- MS/MS for the quantitative proteomic analysis of intes- tine samples from KM mice with different groups and control to discover candidate ...
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Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches
... proteins. iTRAQ has become one of the major quantification tools used in differential proteomic research ...the iTRAQ technique involves a combination of multi- dimensional liquid ...
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Identification of proteins associated with pyrethroid resistance by iTRAQ based quantitative proteomic analysis in Culex pipiens pallens
... vectorbase.org/) [37] using the Mascot software (version 2.3.02, Matrix Science, UK). For protein identification and quantification, a peptide mass tolerance of 10 ppm was allowed for intact peptide masses and ...
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iTRAQ-based proteomic profiling of Vibrio parahaemolyticus under various culture conditions
... as iTRAQ labeling at N-terminus and lysine residues, cysteine modi- fication by Methylmethanethiosulfonate as fixed modifica- tions, and trypsin as a ...were used for further ...LC–MS/MS analysis ...
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Original Article Pathway analysis of esophageal squamous cell carcinoma using iTRAQ-based quantitative proteomic approach
... Received May 25, 2016; Accepted August 4, 2016; Epub October 1, 2016; Published October 15, 2016 Abstract: Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common digestive cancers. Many proteins and ...
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Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots
... the analysis of membrane proteins and resulted in comparable results for protein/peptide iden- tification and reproducibility ...the proteomic workflow furthermore offers the opportunity to determine the pI ...
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<p>Serum Derived Exosomes From Pancreatic Cancer Patients Promoted Metastasis: An iTRAQ-Based Proteomic Analysis</p>
... Although our study has a relatively small sample size and the age gap between patients is large, our results showed that the levels of some differentially expressed proteins from serum-puri fi ed exosomes varied signi fi - ...
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iTRAQ-based proteomic analysis of the reproductive cycle characteristics of Hainan Black Goat, the unique species in China, Hainan Province
... was used to desalt the hydrolyzed peptide fragment. Certain iTRAQ labeling reagents were taken out and added 50 μl isopropanol to each of the tube, then ...was used to dissolve the peptide fragment, ...
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iTRAQ-Based Proteomic Analysis reveals possible target-related proteins and signal networks in human osteoblasts overexpressing FGFR2
... Osteoblasts were harvested on ice in phosphate-buffered saline (PBS) and centrifuged. To prepare cell protein, 10 6 cells were lysed during 15 min on ice in 100 μl of lysis buffer (150mM NaCl, 1% Nonidet P-40, 0.1% SDS, ...
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iTRAQ-based proteomic analysis to identify the molecular mechanism of Zhibai Dihuang Granule in the Yin-deficiency-heat syndrome rats
... commonly used to treat YDH syndrome ...monly used for purging pathogenic fire and expelling ...been used not only in YDH syndrome management, but also to treat the concomitant symp- toms of other ...
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Comparative iTRAQ based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis
... were used to vali- date the proteome by using the activity of cytochrome c oxidase (CCO) and hemocyanin content: 5 for the con- trol and 50 for the experimental ...
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Proteomic Profiling of Cerebrospinal Fluid by 8-Plex iTRAQ Reveals Potential Biomarker Candidates of Alzheimer’s Disease
... Statistical Analysis The iTRAQ reporter ion abundances in MS/MS scans were also evaluated using Protein Pilot ™ Software for relative quantitation of ...peptide used for protein ...
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iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus)
... Acquisition and use of animals were approved by the La- boratory Animal Care Committee of the China Ministry of Health. As described previously by our laboratory [15], nine male Daurian ground squirrels were obtained ...
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