Scratch wound
Royal jelly derived proteins enhance proliferation and migration of human epidermal keratinocytes in an in vitro scratch wound model
... vitro scratch wounding model. The proportion of wound healing induced by Fraction 2 ...the wound scratching assay were reconstituted in serum-free medium which had no effect on cell ...the ...
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A high throughput cell migration assay using scratch wound healing, a comparison of image based readout methods
... two approaches using the results from one 384 well plate of a screen for small molecules that affect wound healing [Figure 4B]. The automated analysis values for each well are plotted and the average lamellar ...
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Measuring cellular migration with image processing
... these scratch wound assays, most of the analysis are limited to the visual observation of a series of microphotographs [2], measurements are generally performed manually, and in the few cases where image ...
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Ellagitannin-rich cloudberry inhibits hepatocyte growth factor induced cell migration and phosphatidylinositol 3-kinase/AKT activation in colon carcinoma cells and tumors in Min mice
... stimulation, scratch wound closure of HT29 and HCA7 cells took over 24 ...by scratch wound healing without HGF treatment in the APC-mutated HT29 cells (Figure 3E; ...accelerated scratch ...
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<p>OGDH promotes the progression of gastric cancer by regulating mitochondrial bioenergetics and Wnt/β-catenin signal pathway</p>
... It was observed that OGDH knockdown BGC823 cells migrated signi fi cantly slower than the control group, and overexpression of OGDH accelerated cell migration through the scratch wound ( [r] ...
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Kunitz type protease inhibitor EgKI-1 from the canine tapeworm Echinococcus granulosus as a promising therapeutic against breast cancer
... time scratch wound assay analysis using the IncuCyte system revealed that ...the scratch wound in control wells decreased eventually with ...The wound width was significantly larger in ...
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Exogenous H2S modulates mitochondrial fusion–fission to inhibit vascular smooth muscle cell proliferation in a hyperglycemic state
... and scratch wound repair assays, decreases proliferative protein (PCNA and cyclin D1) expression, and reduces ROS production in the cytoplasm and ...
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Wound healing activity of standardized brazilin rich extract from Caesalpinia sappan heartwood
... acute. Wound healing is a complex biochemical process where the cell structures are restored to normalcy, which depend on cell proliferation and migration, basically fibroblast ...initiate wound healing ...
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Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1
... using scratch wound ...A scratch wound was generated in the cell monolayer and the degree of wound closure determined 24 hours ...in wound width after 24 hours ± standard error ...
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Upregulation of long noncoding RNA CCAT1-L promotes epithelial–mesenchymal transition in gastric adenocarcinoma
... Objective: In this study, we aimed to investigate the role of a long-chain noncoding RNA, colorectal cancer-associated transcript 1-long (CCAT1-L) in gastric adenocarcinoma. Patients and methods: Expressions of CCAT1-L ...
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A homeopathic remedy from arnica, marigold, St John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts
... cellular wound fill, the “net effect” of all cellular events contribut- ing to the in vitro wound healing process, and has been proven as a valuable tool to obtain first insights into how preparations can ...
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Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repair
... vitro scratch wound assay, we observed that exposure to the ANXA1 mimetic peptide Ac2-26 (3 μM) enhanced wound closure in IECs com- pared with control cells ...on wound closure was confirmed ...
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<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>
... in-vitro scratch wound assay, cell adhesion, cell viability and in-vivo experimental procedures of excision wounds were con- ducted, along with histopathological and immunohisto- chemical studies to con fi ...
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Original Article FL118 inhibited the proliferation of colorectal carcinoma cells via upregulating CDC73
... Linear wound tracks were generated with 10 ul micropipette tip, and discard the non-adherent cell and washed with PBS two ...after scratch- ing was recorded, three independent scratch- wound ...
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A Comparative Topical Wound Healing Study Of 5-Amino Salicylic Acid As Ointment And Gel By In-Vitro And In Vivo Models
... Abstract:- Wound healing is a restoration process of tissue repair in response to an ...the wound healing potential of 5- ASA by was studied by in vitro and by in vivo ...vitro scratch wound ...
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Bioassay-Guided Fractionation of Wound Healing Active Compounds From Piper nigrum L. Berries Extract in Malaysia
... the wound healing property of ...Significant wound healing properties screened using the scratch wound assay was observed through the cell migration assay in fraction number three (PNE3) out ...
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Original Article The role of EphB4 and IGF-IR expression in breast cancer cells
... Cells were plated in 96-well plates with 1000 cells/well in serum-containing medium. Cells were switched to serum-free media (SFM) for 24 h and then treated separately with 10 ng/ml IGF-I for 10 min, 50 µM LY294002 for ...
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Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice
... length, total loops and total branching points compared to controls, and the CM from RANKL + harmine group exhibited much stronger pro-angiogenic effect on HMECs (Figure 2B-D). However, this effect was blocked by ...
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Enhanced Cutaneous Wound Healing by the Leaf Extract of Achillea eriophora D.C. Using the In Vitro Scratch Assay
... reported wound healing activity of Calendula officinalis extract by stimulating in vitro and in vivo proliferation of dermal fibroblasts, highly attributed to the ...
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<p>1,25(OH)<sub>2</sub>D<sub>3</sub> Strengthens the Vasculogenesis of Multipotent Mesenchymal Stromal Cells from Rat Bone Marrow by Regulating the PI3K/AKT Pathway</p>
... 1 µM), the proliferation of MSCs was analyzed by Cell Counting Kit-8 (CCK-8), and the migratory capability was measured by Transwell assays and cell scratch tests. Capillary-like structure formation was observed ...
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