Screening a cDNA library for ACC synthase
Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes
... the library have no known ...the library and their corresponding predicted functions is included as supplementary information [see Additional file ...1]. Screening of transporter cDNA ...
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Identification of stroke associated antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)
... Toshio Machida 1 , Motoo Kubota 2 , Eiichi Kobayashi 3 , Yasuo Iwadate 3 , Naokatsu Saeki 3 , Akira Yamaura 4 , Fumio Nomura 5 , Masaki Takiguchi 6 and Takaki Hiwasa 6* Abstract Background: Because circulating antibodies ...
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Screening of an endothelial cDNA library identifies the C terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations
... By screening a cDNA library from human umbilical artery endothelial cells with serum from an SLE patient with psychosis, we identified one positive strongly reactive clone encoding the C-terminal ...
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Liu, Zhijie (2010): Identification of Novel Genes for the Development of a Rapid Diagnostic Test for Theileria uilenbergi Infection by Screening of a Merozoite cDNA Library. Dissertation, LMU München: Tierärztliche Fakultät
... In einem ersten Verfahren führten PCR Amplifikationen der Genfragmente und anschließende Sequenzierung zu der Entdeckung von 12 einzelnen Klonen. Klone 2, 9 und 26 wiesen einen hohen Grad an Identität auf, besonders am ...
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Screening lambda cdna or genomic libraries
... for cDNA library, ~10 hrs for a genomic ...the library to be plated for screening at a density such that the plaques can grow to about 1 mm in diameter before they begin to just barely touch ...
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Regulation of ethylene biosynthesis via ACC synthase protein stability
... Results Isolation of suppressors of ACS5 overexpressor mutants To find suppressors of ACS5 overexpression, we used a DEX-inducible myc-tagged ACS5 transgenic line that has a ctr-like phenotype when grown in the presence ...
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Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... subtracted cDNA library is obtained, it is impor- tant to confirm that individual clones indeed represent differentially expressed ...tial screening of the subtracted library helps to ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... Arabidopsis cDNA library insertion, but only 35 colonies out of that were able to show blue staining in X-gal ...of cDNA expression it might be concluded that a few proteins have a potential for true ...
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Generation and ethylene production of transgenic carnations harboring ACC synthase cdna in sense or antisense orientation
... ‘Nora’ genomic DNA. The appearance of two DNA fragments in the Hind III digests coincided with the presence of a Hind III cleavage site in the DC-ACS1 genomic DNA, which was deduced from the Hind III cleavage site at the ...
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Generation and ethylene production of transgenic carnations harboring ACC synthase cdna in sense or antisense orientation
... ‘Nora’ genomic DNA. The appearance of two DNA fragments in the Hind III digests coincided with the presence of a Hind III cleavage site in the DC-ACS1 genomic DNA, which was deduced from the Hind III cleavage site at the ...
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In Silico Screening of the Library of Pyrimidine Derivatives as Thymidylate Synthase Inhibitors for Anticancer Activity
... and library design A library of the molecules was generated by performing structural modifications in descending order on five series of pyrimidines each containing 124 ...silico screening flow chart ...
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Identification of immune protective genes of Eimeria maxima through cDNA expression library screening
... The effective antigens also could be masked by the dilu- tion effect during cDELI. For example, if one sub-library contains 100 plasmids during cDELI, each individual plasmid will be delivered in 1/100th of the ...
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ACC Synthase Genes Related to Cold-dependent Ripening in Pear Fruit
... a cDNA clone with homology to an actin sequence (AF386514) was used as an internal control in gene expression ...the cDNA from the highest expressing ...
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Reduced ethylene production in transgenic carnations transformed with ACC oxidase cdna in sense orientation
... carnation ACC synthase (DC-ACS1, s/aACS transgenes) or ACC oxidase (DC- ACO1, s/aACO transgenes) in sense or antisense orientation or mutated carnation ethylene receptor cDNA (DC-ERS2’) by ...
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Profiling of ACC synthase gene (ACS11) expression in Arabidopsis induced by abiotic stresses
... from cDNA and the AtACS11 promoter, including the 1076 bp immediately upstream of the 5 ′ -untrans- lated region of the AtACS11 mRNA was amplified from genomic DNA (Additional file 1: Table ...for cDNA were ...
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Screening of the MayBridge Rule of 3 Fragment Library for trypanocidal compounds that interact with the myo inositol 3 phosphate synthase from Trypanosoma brucei
... Inositol-3-phosphate synthase (INO1) has previously been genetically validated as a drug target against Trypanosoma brucei, the causative agent of African sleeping ...the library are also reported, with the ...
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Exploring the apple genome reveals six ACC synthase genes expressed during fruit ripening
... Fig. 2. Fruit firmness, ethylene production and expression of apple ACS genes in GD fruits. A, Fruit firmness and ethylene production of GD fruits stored at room temperature after harvest. The numbers under the X-axis ...
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Analysis of MaACS2, a stress-inducible ACC Synthase Gene in Musa acuminata AAA Group Cultivar Pisang Ambon
... Quantification of all gene expressions from all samples was performed using SYBR Green as fluorescence dye. Melting curve analysis was done to check for primer dimer formation and specificity of the primers. The melting ...
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Increasing the library size in cDNA display by optimizing purification procedures
... The library size is critical for selection in evolutionary molecular engineering (directed ...Although cDNA display has become a promising in vitro display technology by overcoming the instability of mRNA ...
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ACCOUNTING COURSE QUALITY STANDARDS ACC ACC ACC ACC ACC ACC ACC ACC
... 4.2 Prepare journal entries for treasury stock transactions, using the cost method.. 4.3 Allocate cash dividends between common and preferred stock, in accordance with income sharing [r] ...
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