Screening for GAV using a capture ELISA
Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA
... 3.4. Detection of circulating IL-10 after E. tenella infection IL-10 is a key immunoregulator during infection with pathogens including intracellular protozoa (Gazzinelli et al., 1996; Wilson et al., 2005). As in ...
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Monoclonal antibody based capture ELISA in the diagnosis of previous dengue infection
... vaccination screening in areas where the dengue seroprevalence is > 80% in children aged 9 ...antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the diagnosis of previous dengue ...
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Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA
... Through screening of hybridomas derived from mice immunized with γ-irradiated Nipah virus, we identified two secreted antibodies; one reactive with the nucleocapsid (N) protein and the other, the phosphoprotein ...
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Library screening using ELISA Screening of a PS-SCL for inhibition of mab binding to antigen by ELISA 65
... ONE-BEAD ONE-COMPOUND COMBINATORIAL LIBRARY METHOD approach to identify ligands that bind specifically to yeast (Candida albican) cell surface (unpublished results). In addition to on-bead binding assay, we and others ...
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JE Detect TM IgM ANTIBODY CAPTURE ELISA (MAC-ELISA)
... Dilute the 10X Wash Buffer to 1X using Biological or High-Grade Water. To prepare a 1X wash buffer solution, mix 120 ml 10X wash buffer with 1080 ml distilled (or deionized) water and rinse out any crystals. Swirl ...
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A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial
... The immunization schedule was continued with four more injections at 4-5-day intervals. Five days after the last injection, the spleen of the mouse was removed, spleen cells from the immunized mice were isolated and ...
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ELISA-VIDITEST anti-cmv IgM capture
... h 8. PROCESSING OF RESULTS 8.1 Processing of results for Qualitative screening interpretation 1. Compute the mean absorbance of the two wells of Calibrator (CAL). If Calibrator was applied as triplet and if any of ...
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Screening of metal oxides for Hg0 capture
... calculated using FactSage ...parameters using the reactions of the metal oxides with Hg 0 are summarized in Table ...calculated using FactSage ...
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In silico screening of carbon-capture materials
... CCS. Using this analysis, we have screened hundreds of thousands of zeolite and zeolitic imidazolate framework structures and identified many different structures that have the potential to reduce the parasitic ...
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Microarray ELISA for Autoantibody Screening in Connective Tissue Diseases
... Due to this, the correct diagnosis of ACTD depends not only on the clinical presentation of the patient, but on the determination of the autoantibody profile in the patients’ serum. The first and most essential step in ...
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In house ELISA screening using a locally isolated Leptospirain Malaysia: determination of its cut off points
... available ELISA kits using non-pathogenic ...in-house ELISA using a locally isolated Leptospira strain IMR/175 as the antigen for the detection of Leptospira IgM anti- bodies among patients in ...
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Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogansserovar Copenhageni (M20) derived antigen
... our ELISA IgM assay with a leptospir- osis-negative/HIV-positive serum was identified in this ...on ELISA-IgM testing, in addition to the other diseases included in our negative control ...of ...
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Performance analysis of anaplasma antibody competitive ELISA using the ROC curve for screening of anaplasmosis in camel populations in Egypt
... χ(2) = 41.8, p value ≤ 0.001 and origin: χ(2) = 42.56, p value ≤ 0.001). These may be due to the lack of a proper distribution of health policies in most of the areas and the origin of animals as a source of disease ...
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Evaluation of a commercial Erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples
... 2. Diagnostic methods 2.1. Detection of BVDV by IPX The 220 samples from the field and the 23 samples from PI calves were also tested for BVDV by IPX. The test was carried out on 96-well plates using low-passage ...
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The Screening, Fabrication and Production of Microalgae Biocomposites for Carbon Capture and Utilisation
... Abstract The use of microalgae for carbon dioxide sequestration and as a feedstock for biodiesel production has been a topic of active research since the late 1950s. It has not been adopted as a technology due to the ...
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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
... test (PRNT) which is considered to be the “gold standard technique”. Comparison of VNT and PRNT results The same results (positive/negative) were obtained using CPE- and PCR-based VNTs. Titres were also highly ...
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Establishment of antigen capture ELISA detection method for H9 subtype avian influenza virus HA protein
... By using double antibody sandwich method to establish antigen capture ELISA method have very strict requirements of antibody specificity, and obtain specific antibodies requires high purity of immune ...
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Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito
... antigen capture ELISA for the diagnosis of JEV infection by using the MAb 4D1 against E protein of JEV and the PcAb of JEV derived from the ...proposed ELISA in this report is shown to hold ...
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Copro antigen capture ELISA for the detection of Teladorsagia (Ostertagia) circumcincta in sheep: improvement of specificity by heat treatment
... copro-antigen capture ELISA for the detection of Teladorsagia circumcincta infection in sheep was developed and ...specificity using faeces from animals carrying a range of monospecific infections ...
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Rapid Screening of Toxigenic Vibrio cholerae O1 Strains from South Iran by PCR-ELISA
... ABSTRACT Background: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The ...
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