Semi-quantitative Reverse Transcript Polymerase Chain Reaction
Measurement of bioprocess containment by quantitative polymerase chain reaction
... 1.4.4.2.1 Impactors Impactor samplers operate by causing airborne micro-organisms to deposit on solid or semi-solid surfaces. The surfaces may be an adhesive substance such as gelatin or nutrient agar. If agar is ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... To confirm the specific target amplification, the real-time amplification was followed by melting curve analysis from 65°C to 95°C. Non-template controls (NTC, consisting of nuclease-free H 2 O) were used as negative ...
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Reference genes for quantitative reverse transcription polymerase chain reaction expression studies in wild and cultivated peanut
... We have assessed the stability of ten candidate reference genes for qRT-PCR normalization using an entire data- set and eight samples subsets of leaves and roots from wild relatives and [r] ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The first step in RT-qPCR based gene expression analysis is the faithful conversion of RNA to cDNA by reverse transcription. Therefore, the first step would be the development of an RT enzyme which is able to ...
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Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a LightCycler. The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H 2 O up ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... Modifications to the technique are detailed that preserved visible tissue and cellular structures and improved transcript detection whilst preventing significant generation of artefacts. Background Prior to the ...
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Establishment of real time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene
... time quantitative PCR became an attractive method to study gene expression because of its low inter-assay and intra- assay variablilty and its equivalent or greater analytical sensitivity in comparison with ...
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The combinatorial approach of laser-captured microdissection and reverse transcription quantitative polymerase chain reaction accurately determines HER2 status in breast cancer
... be semi-quantitatively assessed by im- munohistochemistry (IHC) [25, 31, ...real-time polymerase chain reaction (qPCR) has been suggested as simple and cost-effective alter- native to FISH and ...
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A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
... Results High-throughput primer validation procedure A collection of primer pairs from PrimerBank covering most known mouse genes was tested by QPCR, agarose gel electrophoresis, sequencing and BLAST. An overview of the ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and data normalisation were presented in the 'Materials and ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and data normalisation were presented in the 'Materials and ...
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Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases
... 44. Cuadros M, Talavera P, Lopez FJ, Garcia-Perez I, Blanco A, Concha A. Real- time RT-PCR analysis for evaluating the Her2/neu status in breast cancer. Pathobiology. 2010;77:38–45. 45. Baehner FL, Achacoso N, Maddala T, ...
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Gene expression of the renin angiotensin system in human tissues Quantitative analysis by the polymerase chain reaction
... the polymerase chain reaction (PCR) after reverse transcription to detect renin- angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... Fig.28C: Relative fold change of CRBN mRNA level in peripheral blood (PB) mononuclear cells of female patient with with del(5q) and trisomy 8 in different clones during len[r] ...
Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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THE advent of the polymerase chain reaction in the
... Ruane and Austin first identified 21 snakes from museum collections (primarily the California Academy of Sciences and the Museum of Comparative Zoology at Harvard University), representi[r] ...
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Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
... pylon detection by RT-PCR assay, the production of cDNA was performed in a total volume of 30 pl containing PCR buffer with the above concentrations of each deoxynucleotide, 180 ng of HP[r] ...
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One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses
... antibodies, reverse transcription-polymerase chain reaction (RT- PCR), and electron microscopy, all of which have played major roles in the diagnosis of filovirus in- ...
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Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total RNA ...
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