Semi-quantitative reverse transcriptase polymerase chain reaction
Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total RNA ...
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Detection of Norwalk virus in stool specimens by reverse transcriptase polymerase chain reaction and nonradioactive oligoprobes
... For agarose gels, 21 coded stool specimens were purified by Sephadex G-200 spin-column chromatography, amplified by RT-PCR by using Norwalk virus NV-5'Pol and NV-3'Pol primers (left lane[r] ...
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An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
... Abstract Background: To perform a retrospective analysis of patients with IgM antibodies to dengue fever infection to determine the serotypes present by molecular techniques. A representative sample (~20%/per year) of ...
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Canine Distemper Virus detection based in Hemaglutinine Gene as target in Reverse Transcriptase-Polymerase Chain Reaction
... Canine Distemper have become a major concern within the veterinary clinical work. Thus, the appearance of many cases of canine distemper in adult animals with their vaccination plan up to date has alarmed veterinarians. ...
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Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases
... 44. Cuadros M, Talavera P, Lopez FJ, Garcia-Perez I, Blanco A, Concha A. Real- time RT-PCR analysis for evaluating the Her2/neu status in breast cancer. Pathobiology. 2010;77:38–45. 45. Baehner FL, Achacoso N, Maddala T, ...
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Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ...
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Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real time reverse transcriptase polymerase chain reaction to predict recurrence of gastric adenocarcinoma
... The semi-quantitative nature of traditional PCR technol- ogy has made it difficult to differentiate baseline gene expression levels in normal tissues from increased gene expression levels in cancer, thereby ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... 1.4.4.2.1 Impactors Impactor samplers operate by causing airborne micro-organisms to deposit on solid or semi-solid surfaces. The surfaces may be an adhesive substance such as gelatin or nutrient agar. If agar is ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... To confirm the specific target amplification, the real-time amplification was followed by melting curve analysis from 65°C to 95°C. Non-template controls (NTC, consisting of nuclease-free H 2 O) were used as negative ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed ...
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Detection of Rhinovirus, Respiratory Syncytial Virus, and Coronavirus Infections in Acute Otitis Media by Reverse Transcriptase Polymerase Chain Reaction
... AOM, acute otitis media; RSV, respiratory syncytial virus; HRV, human rhinovirus; RT-PCR, reverse tran- scriptase polymerase chain reaction; HCV human coronavirus; NPA, nasopharyngeal as[r] ...
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Deteksi Dan Identifikasi Chrysanthemum Stunt Viroid Pada Tanaman Krisan Menggunakan Teknik Reverse Transcriptase Polymerase Chain Reaction (Detection and Identification of Chrysanthemum Stunt Viroid on Chrysanthemum Using Reverse Transcriptase Polymerase
... Katakunci: Chrysanthemum stunt viroid (CSVd); Reverse transcriptase polymerase chain reaction; Dendranthema grandiflora Kitam; DNA sequencing ABSTRACT. Chrysanthemum stunt viroid (CSVd) ...
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Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real time reverse transcriptase polymerase chain reaction
... PCR reaction started, were determined simultaneously for the messages of both target gene and β-actin gene in the same sample tube when the intensity of the reporter fluorescent signal reached 10 times the ...
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Reference genes for quantitative reverse transcription polymerase chain reaction expression studies in wild and cultivated peanut
... We have assessed the stability of ten candidate reference genes for qRT-PCR normalization using an entire data- set and eight samples subsets of leaves and roots from wild relatives and [r] ...
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A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... Then we constructed and optimized the duplex real-time RT-PCR assay by adjusting the concentrations of primers and probes. Using a series of dilutions of transcripts containing target genes as template, we showed that ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The first step in RT-qPCR based gene expression analysis is the faithful conversion of RNA to cDNA by reverse transcription. Therefore, the first step would be the development of an RT enzyme which is able to ...
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Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a LightCycler. The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H 2 O up ...
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A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... Background and objectives: Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the ...
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The relationship between quantitative human epidermal growth factor receptor 2 gene expression by the 21 gene reverse transcriptase polymerase chain reaction assay and adjuvant trastuzumab benefit in Alliance N9831
... if quantitative levels of the ERBB2 gene ex- pression level as assessed by messenger RNA coding for the HER2 protein quantified by the 21-gene assay and re- ported as the HER2 single gene score are predictive of ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... were less prone to detachment during processing, so these were chosen for the in situ RT-PCR experiments. Whilst the standard de-waxing step was carried out to remove the embedding material, the extensive ...
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