Sequencing primers for the 373A automatic sequencer
Genome Sequencer System. Amplicon Sequencing. Application Note No. 5 / February
... The processing and sequencing of amplicons with the Genome Sequencer System is quite flexible and allows for a wide range of experimental design. A researcher can choose a variety of options regarding ...
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Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
... using sequencing primers with 40–80 bp neutral polynucleotide tails ...tailed primers, however, could in some cases form secondary structures between the tail and the primer 3′ end resulting in a ...
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ABI PRISM 377 DNA Sequencer For Sequencing and GeneScan Analysis Software Applications
... © Copyright 2000, Applied Biosystems For Research Use Only. Not for use in diagnostic procedures. NOTICE TO PURCHASER: This instrument, Serial No. , is Authorized for use in DNA sequencing and fragment analysis. ...
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Evaluation of the Sequencing Pipeline for the Oxford Nanopore MinION Long-read DNA Sequencer
... total sequencing capacity of the MinION was still far below what would be required for an entire human genome sequencing ...the sequencing capacity of the MinION was only likely to increase further ...
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Rigorous Design of Moving Sequencer Atomic Broadcast with Malicious Sequencer
... moving sequencer based atomic broadcast. In moving sequencer mechanisms, there must be some process that is responsible for ...this sequencer will not be fixed for whole ...a sequencer in a ...
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Universal Primers for Detection and Sequencing of Hepatitis B Virus Genomes across Genotypes A to G
... genomes of HBV (12). This is because the primer set amplifies the cccDNA of HBV, instead of the virion DNA. Using a single primer set, the Taq polymerase cannot extend through the virion DNA due to the presence of ...
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Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing based quantification
... exhibit sequencer-specific quantitative bias in gene expression counts, sequencer size bias can influence both the inter- pretation of results and the economics of sequencing in cases where library ...
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Y-200 Multi-Stage Sequencer
... TempTracker Mod+ Hybrid Multi-Boiler Modulating Controller The TempTracker MOD+ Hybrid is available as a loose option and controls up to four MVB, XTherm, XFyre and Raytherm boilers for precise load tracking of space ...
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Genome Sequencer 20 System. First to the Finish.
... Genome Sequencer 20 System across a variety of bacterial genomes are presented in Table ...genome sequencing of bacterial strains up to an x-fold coverage, consensus contigs were generated based on mapping ...
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Automatic DNA sequencing for electrophoresis gels using image processing algorithms
... DNA sequencing can be defined by ...fore, automatic analysis of the gel image could pro- vide more information that is usually ignored by human ...an automatic procedure to analyze DNA gel images ...
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Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers.
... identification of P. aeruginosa, P. cepacia, and P. pickettii, it was found that P. cepacia possessed only minor size polymorphisms but appeared to have considerable sequence polymorphism within the ITS. We observed ...
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A comparison of automatic cell identification methods for single cell RNA sequencing data
... Conclusions We present a comprehensive evaluation of automatic cell identification methods for single-cell RNA sequencing data. Generally, all classifiers perform well across all datasets, in- cluding the ...
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Semi-Automatic Time-Series Transfer Functions via Temporal Clustering and Sequencing
... For future work, the algorithm could become more auto- matic by algorithmically estimating k,w, and γ. If we can integrate spatial filtering and locality into our clustering, se- quence confidence would be increased ...
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Genetic barcoding of marine zooplankton using the MinIon sequencer
... Firstly, all samples are weighed in Eppendorf tubes. For each 10 mg of gel, 10 µL capture buffer type 3 is added. However, if the gel weighs less than 300 mg, still 300 µL is used. The samples are allowed to incubate at ...
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Utility of redesigned cpn60 UT primers and novel fungal specific cpn60 primers for microbial profiling
... genome sequencing for many years. Another first generation sequencing method by Maxam and Gilbert involved nucleobase specific chemical modification of DNA, followed by cleavage of DNA at site adjacent to ...
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AutoPrime: selecting primers for expressed sequences
... While automatic generation of primers for expressed sequences is possible, current primer design software is inable to cope with the positional restrictions imposed by the quality requirements for RT-PCR ...
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Rigorous Design of moving Sequencer Omission Tolerant Atomic Broadcast
... moving sequencer based atomic broadcast. In moving sequencer mechanisms, there must be some process that is responsible for ...this sequencer will not be fixed for whole ...a sequencer in a ...
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A Semantic Web Annotation Tool for a Web-Based Audio Sequencer
... The project has followed web standards and principles with the goal of making music compositions part of the Semantic Web. Future plans consist in extending the tool by developing new user interfaces to annotate other ...
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DNA SEQUENCING (using an ABI automated sequencer)
... Add approximately 5 volumes of the PB buffer (Qiagen DNA purification kit) to each tube. Mix thoroughly and return to the water bath. Place one Qiagen spin column into the Collecti[r] ...
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DewesoftX SEQUENCER SOFTWARE USER MANUAL. Sequencer V20-1
... In the property panels we need to enter the Tab and the Button name. The TabName is the name of the main tabs of DewesoftX® . In our case I want the software to go to Ch. Setup – Storing screen that the user can enter ...
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