Serine Proteinase
A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro.
... the serine proteinase (4A/4B, 4B/5A, and 5A/5B) can occur in trans (3, 9, 26), but it is unknown if they can also occur in ...the serine proteinase—a membrane- bound NS3-NS4A complex and ...
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Purification and Characterization of a 43 Kilodalton Extracellular Serine Proteinase from Cryptococcus neoformans
... Proteins secreted by fungal pathogens could be involved in their invasive process and might be useful in vaccine design (4). The identification and characterization of the C. neoformans extracellular protein is important ...
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Substrate requirements of hepatitis C virus serine proteinase for intermolecular polypeptide cleavage in Escherichia coli.
... 11 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Substrate Requirements of Hepatitis C Virus Serine Proteinase for Intermolecular Polypeptide Cleavage in Esch[r] ...
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The serine proteinase hepsin is an activator of pro-matrix metalloproteinases : molecular mechanisms and implications for extracellular matrix turnover
... implicates serine proteinases in the proteolytic cascades leading to the pathological destruction of extracellular matrices such as cartilage in osteoarthritis ...transmembrane serine proteinase ...
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Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics.
... 23, the pestivirus plO protein may be the functional homolog of HCV NS4A, because sequences in this region of the pestivirus polyprotein appear to be required for the serine proteinase-d[r] ...
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Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.
... the serine proteinase, a mutant containing an alanine substitution at position 1842 (called pACNR/ NADL S1842A) was constructed by replacing the BglI (nucleotide 6263; NADL numbering [19]) to NsiI ...
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The endogenous vascular elastase that governs development and progression of monocrotaline induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin
... The Endogenous Vascular Elastase That Governs Development and Progression of Monocrotaline-induced Pulmonary Hypertension in Rats Is a Novel Enzyme Related to the Serine Proteinase Adips[r] ...
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Characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the A bisporus SPR1 Promoter
... Foster Article Title: Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A... Characterisation of se[r] ...
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Orthopoxvirus fusion inhibitor glycoprotein SPI-3 (open reading frame K2L) contains motifs characteristic of serine proteinase inhibitors that are not required for control of cell fusion.
... The cowpox virus (CPV) SPI-3 gene (open reading frame K2L in vaccinia virus) is one of three orthopoxvirus genes whose products are members of the serpin (serine proteinase inhibitor) superfamily. The CPV ...
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Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi
... the serine, cys- teine, and metalloproteinases of Acanthamoeba were pub- lished (9, 12, 18, ...19). Serine proteinase purified from culture supernatant was reported to play roles in host tissue ...
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Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba
... the serine protease inhibitor PMSF ...of proteinase activity on cyst formation, cysteine proteinase inhibitor (E64) or serine proteinase inhib- itor (PMSF) was added to trophozoites in ...
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Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
... Amino acids substitutions to asparagine at both the P1 and P1' positions of the putative 3/4A, 4A/4B, 4B/5A cleavage sites of the nonstructural precursor polyprotein were independently i[r] ...
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Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites.
... To further refine the map of the HCV nonstructural proteins and to determine the sequence at the four potential NS3 proteinase cleavage sites, the N-terminal sequences of NS4A, NS4B, NS5[r] ...
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A new fibrinogenase from Echis multisquamatis venom is a perspective agent for limited proteolysis and defibrinogenation
... A serine proteinase with a fibrinogenase activity was isolated from the venom of viper Echis multisquama- tis. Isolation was performed by the combination of Q-sepharose and Heparine-agarose chromatography. ...
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Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection
... prophenoloxidase, serine proteinase-like protein, heat shock protein 90 and alpha-2- macroglobulin, and those involved in signal transduction, such as the14-3-3 protein epsilon and ...
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Structure function study of a plant protein proteinase inhibitor: site directed mutagenesis of Cucurbita maxima trypsin inhibitor V
... The structure of CMTI-V is also known and is similar to structures of other serine proteinase inhibitors of the Potato I family, e.g., chymotrypsin inhibitor from barley McPhalen and Jam[r] ...
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Purification and characterization of trypsin like protease from the mid guts of Helicoverpa armigera
... that serine proteinase especially trypsin like protease was predominant in midguts of fifth instar ...by serine proteinase inhibitors SBTI (soybean trypsin inhibitor) and TLCK, which are ...
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Fibroblast activation protein alpha is expressed by chondrocytes following a pro inflammatory stimulus and is elevated in osteoarthritis
... Using an active-site probe, we have identified for the first time active FAP α , a serine proteinase on the chondrocyte mem- brane. We have shown that FAP α gene expression is up-reg- ulated by ...
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Fenofibrate in the management of AbdoMinal aortic anEurysm (FAME)-2: the study protocol for a multi-centre, randomised, placebo-controlled trial
... Kallistatin is a serine proteinase inhibitor, known to be expressed in endothelial cells and vascular smooth muscle cells.33 Overexpression of human kallistatin in rodent models has been[r] ...
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Original Article Cyclosporine A improves adhesion and invasion of mouse preimplantation embryos via upregulating integrin β3 and matrix metalloproteinase-9
... implantation serine proteinase 1 (ISP1), integrin (itg) β3 and matrix metalloproteinase (MMP)-9 were determined by real time PCR and immunofluorescence, ...
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