serovar hardjo
Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo bovis provides a sensitive diagnostic probe for bovine leptospirosis
... interrogans serovar hardjo type hardjo-bovis lanes 1, serovar hardjo type hardjoprajitno lanes 2, serovar pomona type kennewicki lanes 3, serovar grippotyphosa lanes 4, serovar copenhage[r] ...
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A Leptospira borgpetersenii Serovar Hardjo Vaccine Induces a Th1 Response, Activates NK Cells, and Reduces Renal Colonization
... Fluorescent antibody detection. Detection of Leptospira in urine and tissue was done by fluorescence microscopy following direct staining of bacteria with high-titer rabbit anti-Leptospira sera conjugated with ...
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Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms
... Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR AP-PCR fingerprinting, and the study of mapped[r] ...
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Repetitive sequences cloned from Leptospira interrogans serovar hardjo genotype hardjoprajitno and their application to serovar identification
... A series of Southern blot experiments, using 12 probes as one to four copies in the hardjoprajitno genome, confirmed the results obtained with pLl and pL590: a high degree of homology wa[r] ...
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Use of Fluorescent Amplified Fragment Length Polymorphism for Molecular Epidemiology of Leptospirosis in India
... Laboratory diagnosis and criteria for confirmation. The criteria for laboratory confirmation were based on either successful isolation of leptospires from clinical specimens or positive serology by a microscopic ...
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Use of Recombinant Flagellin Protein as a Tracer Antigen in a Fluorescence Polarization Assay for Diagnosis of Leptospirosis
... Information on the nature and identity of leptospiral anti- gens is important for elucidation of their significance in the immunity to leptospirosis and the pathogenesis and diagnosis of leptospirosis. Leptospires have a ...
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Schmid, Martin (2005): Prävalenz von Leptospirenantikörpern in bayerischen Rinderherden und von Leptospiren bei abortierten Rinderfeten. Dissertation, LMU München: Tierärztliche Fakultät
... Die Angaben der Fragebögen wurde zunächst für die EDV (Microsoft Access ® ) erfasst, anschließend zu den Herden in Bezug gesetzt und einer statistischen Bewertung unterzogen. Als serologisch positive Betriebe wurden alle ...
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Evaluation of a commercial enzyme linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection
... with serovar hardjo, 18 infected with serovar pomona, 7 in- fected with serovar copenhageni, and 1 infected with serovar australis), diag- nosed on the basis of the MAT and/or culture, ...
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Detection of leptospires in urine by polymerase chain reaction
... Clone pLBEc23s showed a strong hybridization with its homologous serovar hardjo, type hardjobovis, and with serovars balcanica, sejroe, polonica, dikkeni, nyanza, and saxkoebing.. Weaker[r] ...
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Roczek, Alexandra (2008): Entwicklung einer quantitativen PCR zum Nachweis von DNA pathogener Leptospiren in Glaskörper- und Kammerwasserproben von Pferden. Dissertation, LMU München: Tierärztliche Fakultät
... Werden beispielsweise E. coli, als Prototyp der gramnegativen Bakterien, für rund zehn Minuten gekocht, platzen die Bakterien. Bei einer anschließenden Zentrifugation löst sich die DNA aus den denaturierten Zelltrümmern. ...
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Relationship among selected Leptospira interrogans serogroups as determined by nucleic acid hybridization
... interrogans serogroup Sejroe serovar hardjo strain Hardjoprajitno, serogroup Grippotyphosa serovar grippotyphosa strain Andaman, serogroup Icterohaemorrhagiae serovar copenhageni strain [r] ...
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Comparison Study Of Toxicity Bacillus thuringensis from Soil Isolate and Mulberry Leaves in Indonesia
... The name proposed of Bacillus thuringiensis for a species of bacillus which was isolated from diseased larvae of Mediterranean flour moth Angasta (Ephestia) kuhniella Zell [3]. Later, noted infection of the larvae after ...
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Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia
... are repeated DNA sequences of varying copy number. They are caused by slipped strand mispairing during DNA replication [24,25]. VNTRs can provide information relat- ing to both the evolutionary and functional areas of ...
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Molecular Follow Up of Salmonella enterica subsp enterica Serovar Agona Infection in Cattle and Humans
... PFGE profiles. Double bands, i.e., bands containing two frag- ments of roughly the same size but visible as one thick band, are commonly produced from serovar Agona by digestion with XbaI. The presence of plasmids ...
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Evaluation of Three Newly Developed Enzyme Linked Immunosorbent Assays and Two Agglutination Tests for Detecting Salmonella enterica subsp enterica Serovar Dublin Infections in Dairy Cattle
... of serovar Dublin can be extrapolated to the serology of latent carriers of serovar ...shedding serovar Dublin after they were housed separately from the active ...any serovar Dublin-positive ...
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Coinfection with Haemophilus parasuis serovar 4 increases the virulence of porcine circovirus type 2 in piglets
... In addition, the 70 kDa heat-shock proteins (Hsp70) is one of the most important heat shock proteins, it has been proposed as one of the candidate vaccine antigens of Mhp [48, 49], we have found the Hsp70 was up- ...
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aroA Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant
... ABSTRACT Recombinant attenuated Salmonella enterica serovar Typhimurium strains are believed to act as powerful live vac- cine carriers that are able to elicit protection against various pathogens. Auxotrophic ...
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Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells
... parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) ...
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Serological studies and isolations of serotype hardjo and Leptospira biflexa strains from horses of Argentina
... The very limited number of leptospiral isolations of the less fastidious pathogenic serotypes, viz., pomona or canicola, that have been reported in horses, and the few pathogenic strains[r] ...
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Discrimination within Phenotypically Closely Related Definitive Types of Salmonella enterica Serovar Typhimurium by the Multiple Amplification of Phage Locus Typing Technique
... of serovar Typhimurium ...of serovar Typhimurium phage types as well as other ...of serovar Typhimurium phenotypically that were closely related by lysis pattern, in ...
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