Shiga-like toxin
Evaluation of oligonucleotide probes for identification of shiga like toxin producing Escherichia coli
... Four synthetic oligonucleotide probes representing different regions of the Shiga-like toxin I SLT-I structural genes and one oligonucleotide derived from the SLT-II gene of Escherichia [r] ...
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Processing and trafficking of Shiga like toxin 1 in eukaryotic cells
... 1993 Proteolytic cleavage at arginine residues within the hydrophilic disulphide loop of the Escherichia coli Shiga-like toxin 1 A subunit is not essential for cytotoxicity.. Calderwood [r] ...
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Genome sequences of the Shiga-like toxin-producing Escherichia coli NCCP15655 and NCCP15656
... the Shiga toxin and the LEE island harboring the type III secretion system are the major features of EHEC strains ...the Shiga-like toxin, but not genes related to the LEE ...
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Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Macrophage Colony-Stimulating Factor (hGM-CSF) in Escherichia coli and Its Recognition by Reciprocal Antibodies
... of Shiga-like toxin and hGM-CSF was ...of Shiga-toxin is located at the 3’-end of the gene and the carboxy terminus of the protein ...of Shiga-toxin in place of the ...
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Purification of Shiga-like Toxin from Escherichia coli O157: H7 by a Simple Method
... Stx-like toxin causes gastrointestinal symptoms including diarrhea and intestinal ...This toxin is a hexamer protein with a molecular weight of ...the Shiga-like toxin, which can ...
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A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A2 and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model
... Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC) strains are important food-borne pathogens rep- resenting the major etiological agents of hemorrhagic colitis and hemolytic uremic ...
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Shiga-Like Toxin from Local Isolates of Escherichia
... Shiga-like toxin proven to trigger T47D cell entering apoptosis, late apoptosis, and necrosis stages. 164[r] ...
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Close association of verotoxin (Shiga like toxin) production with enterohemolysin production in strains of Escherichia coli
... coli strains of O group 26 were positive for enterohemolysin than for verotoxin production 4; unpublished data and by the finding that VT+ strains of O groups 113 and 114 were hemolysin [r] ...
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Cytosolic entry of Shiga like toxin A chain from the yeast endoplasmic reticulum requires catalytically active Hrd1p
... the toxin subunit to be perceived as ‘misfolded’ by ER quality control ...both toxin subunits, Hrd1p L74 is strongly required for RTA dislocation but has no obvious role for ...
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The biological properties of Shiga like toxin I
... Shiga toxin and the Escherichia coli Shiga-like toxins SLTs are type 2 ribosome inactivating proteins RIPs, exhibiting a specific RNA N-glycosidase activity comparable to that of the pla[r] ...
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Development and evaluation of enzyme linked immunosorbent assays for detection of shiga like toxin I and shiga like toxin II
... Although the SLT-II ELISA was specific for the SLT-II variant produced by porcine edema strains, most of the isolates examined produced levels of toxin 50 CDo per ml in E.. coli culture [r] ...
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Rapid method to detect shiga toxin and shiga like toxin I based on binding to globotriosyl ceramide (Gb3), their natural receptor
... Because these well-defined toxins are emerging as the most important and clinically relevant cytotoxins produced by members of the family Enterobacteriaceae, we have developed a rapid di[r] ...
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Detection of Shiga toxin producing Shigella dysenteriae type 1 and Escherichia coli by using polymerase chain reaction with incorporation of digoxigenin 11 dUTP
... A technique has been developed for the detection of Shiga toxin- and Shiga-like toxin type I ShT/SLT-Iproducing Shigella dysenteriae type 1 and Escherichia coli by using the polymerase c[r] ...
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Comparative Genome Analysis of Three Pathogenic Strains of E. coli, Salmonella and Shigella
... The comparative analysis of coding regions of virulence factor suggests that Shiga-like toxin Stx1A and Stx1B of E.coliO157:H7 str sakai were 99% similar with stxA and stxB of Shigella dysenteriae ...
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Comparative Genome Analysis of three Pathogenic Strains of E. Coli, Salmonella and Shigella
... The comparative analysis of coding regions of virulence factor suggests that Shiga-like toxin Stx1A and Stx1B of E.coliO157:H7 str sakai were 99% similar with stxA and stxB of Shigella dysenteriae ...
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Sensitive method for detecting low numbers of verotoxin producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin
... The reference VTEC strain H.30 classified as a high-level producer of Shiga-like toxin [17] and strains RDEC-1, E74/68, and E851/71 classified as low-to-moderate producers of Shiga-like [r] ...
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Experimental non O group 1 Vibrio cholerae gastroenteritis in humans
... In this study, 27 volunteers received one of three non-O group 1 Vibrio cholerae strains in doses as high as 10(9) CFU. Only one strain (strain C) caused diarrhea: this strain was able to colonize the gastrointestinal ...
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Verocytotoxin expression in Escherichia coli
... strains were used in neutralisation assays (Head et al., 1988b). Also Dickie et al. (1989) found that antisera from VT2 producing strain 3657 (serogroup C157:H7) and K12 (pEBl) could not completely neutralise the VT ...
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Rapid Detection of Enterohemorrhagic Escherichia coliby Real Time PCR with Fluorescent Hybridization Probes
... 14. Paton, J. C., and A. W. Paton. 1998. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11:450–479. 15. Riley, L. W., R. S. Remis, S. D. Helgerson, H. ...
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A Poly N Acetylglucosamine−Shiga Toxin Broad Spectrum Conjugate Vaccine for Shiga Toxin Producing Escherichia coli
... Shiga toxins are the principal cause of the diarrhea and HUS associated with STEC infections (8). They are categorized into two antigenically distinct groups, Stx1 and Stx2, which are potent cy- totoxins composed ...
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