Shiga-Toxin Producing E. Coli (STEC)
Disease severity of Shiga toxin-producing E. coli O157 and factors influencing the development of typical haemolytic uraemic syndrome: a retrospective cohort study, 2009–2012
... The fi nal multivariable model identi fi ed those cases diagnosed by serological testing as at an increased risk of tHUS. This is most likely due to reversed causality, as serological testing is more frequently undertaken ...
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Prevalence and distribution of the stx1, stx2 genes in Shiga toxin producing E. coli (STEC) isolates from cattle
... The seasonal shedding of STEC and distribution of stx ranged from 8.69 to 40.9%. There are significant seasonal differences in the levels of shedding of stx gene-carrying bacteria. The results revealed decrease in ...
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Usability and Performance of CHROMagar STEC Medium in Detection of Shiga Toxin Producing Escherichia coli Strains
... CHROMagar STEC medium to grow Shiga toxin-produc- ing and other diarrheagenic ...E. coli strains was investigated by using a deep- frozen collection of STEC, enteropathogenic ...
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Characterization of Shiga toxin-producing Escherichia coli (STEC) in feces of healthy and diarrheic calves in Urmia region, Iran.
... pathogenic E. coli strains, which typically carry certain virulence gene patterns associated with specific pathotypes (1), commensal ...E. coli strains rarely contain virulence genes ...called ...
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... of shiga toxin producing ...the STEC isolates, RAPD fingerprinting assay was used [17,18] in which PCR was performed at least three times by different technicians in 50μl reaction volumes ...
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Shiga Toxin Producing Escherichia coli in Children: Diagnosis and Clinical Manifestations of O157:H7 and Non O157:H7 Infection
... of STEC are that laboratories perform both a culture for specific detection of O157:H7 and an assay for Shiga toxins ...for E. coli O157:H7 by culture, only 3% used a Shiga toxin ...
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Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC
... for STEC and for use in the development of a sensitive and specific immunodiagnostic assay for the detec- tion of STEC that would avoid expensive reagents and equip- ...the STEC strains and its non- ...
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Shiga Toxin Producing Escherichia coli: a Single Center, 11 Year Pediatric Experience
... of Shiga toxin-producing Escherichia coli (STEC) in chil- dren with diarrheal illness treated at a tertiary care center, ...for Shiga toxin, or the simultaneous use of ...
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A Poly N Acetylglucosamine−Shiga Toxin Broad Spectrum Conjugate Vaccine for Shiga Toxin Producing Escherichia coli
... microbes producing the native, highly acetylated PNAG (24), including a variety of ...E. coli strains isolated from the urinary tract ...against STEC or any other pathogen confined to the gas- ...
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9-2-2015 12:00 AM A Plant-Made Vaccine Candidate To Protect Ruminants Against Shiga Toxin-Producing Escherichia Coli
... to E. coli and efficiency is dependent on translation initiation, elongation, and termination (Lithwick and Margalit, 2003; Sugiura, ...between E. coli and N. tabacum plastids, all ...
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Antibiotic Resistance, Virulence Gene, and Molecular Profiles of Shiga Toxin Producing Escherichia coli Isolates from Diverse Sources in Calcutta, India
... of STEC is complicated by its ubiquitous nature and lack of heterogeneity between clonal ...of E. coli causing enterohemorrhagic disease is restricted by clonal strains that are responsible for the ...
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Genotypes and phenotypes of Shiga toxin-producing <em>Escherichia coli</em> (STEC) in Abeokuta, Southwestern Nigeria
... STEC-infected individuals cannot be overemphasized as it further complicates the disease condition through the release of toxins from the Gram-negative wall of the bacterium. It further clarifies that the role of ...
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Role of antigen-43 on biofilm formation and horizontal antibiotic resistance gene transfer in non-O157 Shiga toxin producing Escherichia coli strains
... by E. coli and other bacteria such as Listeria monocytogenes and Yersinia entero- colitica (17, ...of E. coli to glass (17). It has long been known that E. coli can adhere to ...
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Development of a Rapid PCR Method Using the Insertion Sequence IS1203 for Genotyping Shiga Toxin Producing Escherichia coli O157
... The genomes of most bacteria contain distinct classes of insertion sequences (IS). IS typing involves utilizing the diver- sity of insertion sites. For some bacterial species, IS typing can be utilized for genotyping ...
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Genetic Heterogeneity of Shiga Toxin Producing Escherichia coli Strains Isolated in São Paulo, Brazil, from 1976 through 2003, as Revealed by Pulsed Field Gel Electrophoresis
... the STEC strains that were of different serotypes and that were isolated in Sa ˜o Paulo between 1976 and ...among STEC strains in Fin- land and showed a high degree of similarity among O26:H11 STEC ...
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Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans
... methods. STEC isolates were serotyped according to Bockemühl et ...against E. coli O antigens 1 to 173 and H antigens 1 to ...[verotoxin-producing E. coli reverse passive latex ...
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Evaluation of Enzyme Immunoassays and Real Time PCR for Detecting Shiga Toxin Producing Escherichia coli in Southern Alberta, Canada
... negative-control STEC stool samples were included for extraction and amplification; and positive DNA and water controls were incorporated for ...(E. coli O157 Sakai strain) containing from 10 8 cells ...
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Evaluation of CHROMagar STEC and STEC O104 Chromogenic Agar Media for Detection of Shiga Toxin Producing Escherichia coli in Stool Specimens
... reported STEC outbreak in 1982 (12), various methods for the detection of STEC, especially ...E. coli O157: H7, which is the most prevalent group of STEC, have been devel- oped ...(13). ...
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Shiga Toxin 1c Producing Escherichia coli Strains: Phenotypic and Genetic Characterization and Association with Human Disease
... harboring E. coli isolates belonged to 22 serotypes, and each produced Stx1c as demonstrated by latex ...an STEC autoagglutinating ...Stx1c-producing E. coli isolates were mostly ...
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Subtyping Method for Escherichia coli Shiga Toxin (Verocytotoxin) 2 Variants and Correlations to Clinical Manifestations
... by Shiga toxin-producing Escherichia coli (STEC), alternatively known as verocytotoxin-produc- ing ...E. coli, account for the most severe symptoms among those caused by ...
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