short hairpin
Review on: Short Hairpin Ribonucleic Acid (RNA)
... 25-nucleotide short interfering RNAs (siRNAs) are the key effector molecules of RNAi in Caenorhabditis elegans ...encoded short hairpin RNAs ...
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Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
... antiviral short interfering RNAs (siRNAs), we established a plasmid-based test system express- ing the mRNA of DsRed2 fused with mRNA sequences of single viral genes, to which certain siRNAs were ...as ...
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Short hairpin RNA targeting 2B gene of coxsackievirus B3 exhibits potential antiviral effects both in vitro and in vivo
... Methods: To evaluate the impact of RNA interference on viral replication, cytopathogenicity and animal survival, short hairpin RNAs targeting the viral 2B region shRNA-2B expressed by a [r] ...
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Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery
... Abstract: Two different disulfide (SS)-containing poly(amidoamine) (PAA) polymers were constructed using guanidino (Gua)-containing monomers (ie, arginine [Arg] and agmatine [Agm]) and N,N′-cystamine bisacrylamide (CBA) ...
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Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA
... by short hairpin RNAs (shRNAs), is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNAs (dsR- NAs) by sequence-specific post-transcriptional silencing of homologous ...
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Selective gene silencing by viral delivery of short hairpin RNA
... of short double- stranded (ds) interfering RNA oligonucleotides (siRNA), where the gene silencing effect is only transient in nature, and possibly not suitable for all applications; or ii) nuclear delivery of gene ...
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Short hairpin looped oligodeoxynucleotides reduce hepatitis C virus replication
... Background: Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Standard therapy consists of a combination of interferon-alpha and ...
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Short hairpin loop structured oligodeoxynucleotides reduce HSV 1 replication
... ble-stranded hairpin loop-structured ODNs with one strand completely complementary to the target mRNA – can reduce HSV-1 replication in ...that hairpin loop- structured ODNs have an antiviral effect on ...
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The NPR1 ortholog PhaNPR1 is required for the induction of PhaPR1 in Phalaenopsis aphrodite
... using short hairpin sequences to examine whether it is required for PhaPR1 transcript accumulation during virus infection and resistance against ...PhaNPR1 short hairpin sequence, the tran- ...
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Silencing B7-H1 enhances the anti-tumor effect of bladder cancer antigen-loaded dendritic cell vaccine in vitro
... containing short hairpin RNA (shRNA) sequence aimed at B7-H1 and then infected DCs to investigate whether B7-H1 knockdown could augment the immune function of DCs and further elicit a more potent anti-tumor ...
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Depletion of Kcnq1ot1 non coding RNA does not affect imprinting maintenance in stem cells
... chromosome. Short hairpin RNA (shRNA)- mediated Kcnq1ot1 RNA depletion in embryonic stem (ES), trophoblast stem (TS) and extra-embryonic endoderm stem (XEN) cells had no observable effect on imprinted gene ...
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Therapeutic benefit derived from RNAi mediated ablation of IMPDH1 transcripts in a murine model of autosomal dominant retinitis pigmentosa (RP10)
... exceedingly mild retinopathy, with electroretinographic (ERG) waveforms still detectable and the bulk of the outer nuclear layer (ONL) of the retina remaining intact, at 2 years of age (12). These data strongly suggest ...
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Human Cytomegalovirus Infection Induces Adipocyte-Like Lipogenesis through Activation of Sterol Regulatory Element Binding Protein 1
... using short hairpin RNA (shRNA) showed that SREBP1 cleavage and the induction of lipogenic genes and lipid synthesis are all inhibited in HCMV-infected ...
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<p>New Aspects of Ultrasound-Mediated Targeted Delivery and Therapy for Cancer</p>
... as an encapsulated shell to protect siRNA from being degraded by ribonucleases, meanwhile in combination with ultrasound microbubbles to synergistically transfect VEGF-siRNA. Results showed signi fi cantly higher siRNA ...
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Original Article A RTK-based functional RNAi screen reveals determinants of PTX-3 expression
... a short hairpin RNA (shRNA) library representing the full comple- ment of 56 human RTKs (Table 1) for genes whose inhibition could impair PTX-3 expression in U937 ...
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Adenovirus VA1 Noncoding RNA Can Inhibit Small Interfering RNA and MicroRNA Biogenesis
... Artificial short hairpin RNAs (shRNAs) (the structures of shRNAs are very similar to the structures of pre-miRNAs) have been widely used as precursors of siRNAs in ...
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<p>CircCDYL Serves as a New Biomarker in Mantle Cell Lymphoma and Promotes Cell Proliferation</p>
... Methods: In this study, we detected the expression level of circCDYL in the plasma of MCL patients compared to healthy donors by the quantitative reverse transcription polymer- ase chain reaction. The diagnostic value of ...
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Enhancement of Hepatitis B Virus Replication by Androgen and Its Receptor in Mice
... (AR) short hairpin RNA (shRNA) into the liver of naïve mice by hydrodynamic injection revealed that the effect of androgen on HBV was dependent on its receptor, which apparently could also stimulate HBV ...
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Polycomb recruitment attenuates retinoic acid induced transcription of the bivalent NR2F1 gene
... by short hairpin RNA (shRNA) technology enhanced the RA-associated transcription of Nr2F1, Nr2F2, Meis1, Sox9 and BMP2, but had no effect on the Hoxa5, Hoxa1, Cyp26a1, Cyp26b1 and RARb 2 transcript levels ...
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Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma
... Two NPC cell lines, TW01 (provided by CH Tsai, National Taiwan University, Taiwan) and BM1 (provided by YS Chang, Chang Gung University, Taiwan), were used [53]. All cell lines were maintained in Dulbecco’s modified ...
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