Signal peptides
Unexpected Diversity of Signal Peptides in Prokaryotes
... the signal peptide cleavage motifs above, which originated from bottom-up proteomics data, with intact protein mass spectrometry ...the signal peptide cleavage (see Materials and ...identified signal ...
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Signal peptides for recombinant protein secretion in bacterial expression systems
... universal signal peptide exists that promotes the best possible secretory production of any desired target proteins in any chosen bacterial expression ...fitted signal peptide has to be identified for every ...
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Extensive sequence turnover of the signal peptides of members of the GDF/BMP family: exploring their evolutionary landscape
... of signal peptides within the same phylogenetic ...the signal peptide (BMP4), passing through cases of overall conservation excluding the signal peptide (GDF8), to cases of rather low ...
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Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli
... Large volume fed-batch cultures were performed in a DasGip fermentor (SaniSure Inc, Moorepark, CA, USA) with 1 L working volume. Feed solutions, cul- ture medium and other supplements were prepared as previously ...
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A Genetic Programming Method for the Identification of Signal Peptides and Prediction of Their Cleavage Sites
... A signal peptide is a short region of amino acid residues situated at the N-terminal part of some peptide ...monly, signal peptides are referred to as the address tags within the cell since they ...
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Stable Signal Peptides and the Response to Secretion Stress in Staphylococcus aureus
... intact signal peptides (6, 7), cleaved not at the SPase cleavage site, but rather within the signal peptide itself ...intact signal peptide ...intact signal peptides affect the ...
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Original Article Molecular characterization of the group 9 allergen of Dermatophagoides farinae
... a signal peptides sequence from 1 to 24 amino acid residues, after removal of this sequence, the active part of Der f 9 should consist of 226 amino acid residues with a calculated molecu- lar weight of ...
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An Improved Approach for Rapidly Identifying Different Types of Gram Negative Bacterial Secreted Proteins
... N-terminal signal peptides, and a 41-dimensional vector is used to describe each protein ...N-terminal signal peptides, the predictive ability of SVM models for discriminating CSPs from NCSPs ...
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C-Terminal Signals Regulate Targeting of Glycosylphosphatidylinositol-Anchored Proteins to the Cell Wall or Plasma Membrane in Candida albicans
... short peptides from the N and C termini of the ...C-terminal signal peptides from Hwp1p to target the GFP reporter to the cell surface, (ii) to identify the site in Hwp1p, and (iii) to assess the ...
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Improving extracellular production of Serratia marcescens lytic polysaccharide monooxygenase CBP21 and Aeromonas veronii B565 chitinase Chi92 in Escherichia coli and their synergism
... Signal peptides showed large variations in secretion effi- ciencies, depending on the nature of the secreted ...suitable signal peptide for one protein may not be effi- cient for another protein (Low ...
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Non-contiguous finished genome sequence and description of Bartonella senegalensis sp. nov.
... Open reading frames (ORFs) were predicted using PRODIGAL [34] with default parameters, but pre- dicted ORFs were excluded if they spanned a se- quencing gap region. The predicted bacterial pro- tein sequences were ...
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Apoplastic recognition of multiple candidate effectors from the wheat pathogen Zymoseptoria tritici in the nonhost plant Nicotiana benthamiana
... secretion signal peptide (+ SP); expression of MgNLP lacking the secretion signal ( SP) did not induce cell death ...without signal peptides, to test the require- ment for apoplastic ...
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The Use of UniProtKB/BIOPEP for the Analysis of Oat Globulin Physicochemical Parameters and Bioactivity
... In silico hydrolysis of oat globulins with selected enzymes (BIOPEP). In silico proteolysis of 12 globulins (six complete proteins and the same number of pro- teins from which signal peptides were removed) ...
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Multicenter Evaluation of a Sequence Based Protocol for Subtyping Shiga Toxins and Standardizing Stx Nomenclature
... Nucleotide sequences of the full stx operon that encode the signal peptides 66 bp in the A and 60 and 57 bp, respectively, in the B subunits of Stx/Stx1 and Stx2, the A subunit 879 bp in[r] ...
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5586.pdf
... with signal peptides and powers their translocation through the SecYEG membrane ...native signal peptide is not only SecA2-dependent, as we observed before, but it is also influenced by the Tat ...
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Evaluation of MERTK evolution and efferocytosis signalling
... MERTK’s signal peptide and transmembrane domain, absent from TYRO3 and ...ancestral signal peptides revealed three nonsynonymous mutations in humans, with a G14C mutation producing a potential non-B ...
134
Humoral and cellular responses to a non adjuvanted monovalent H1N1 pandemic influenza vaccine in hospital employees
... To evaluate the proliferative response after pandemic A/H1N1 vaccination, we examined the percentage of pro- liferating peripheral CD4 + and CD8 + T cells following stimulation with H1N1 peptides. This analysis ...
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Recognition of nucleotides and peptides using designed betahairpin peptides and dynamic combinatorial chemistry
... The peptides were dissolved in either 20 mM Phosphate Buffer, pH ...The peptides were combined in equal ratios (100 µM each) and allowed to equilibrate via air oxidation (Figure ...
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Alteration of Substrate and Inhibitor Specificity of Feline Immunodeficiency Virus Protease
... of peptides. FIV and HIV-1 junction peptides represent the primary sequences from the viral cleavage sites (see Table ...library peptides are based on the primary sequences of the random hexamer ...
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Toward a catalog for the transcripts and proteins (sialome) from the salivary gland of the malaria vector Anopheles gambiae
... a signal peptide (Nielsen et al., 1997). In the event a signal peptide was predicted to exist, the molecular mass and the pI of the mature protein were also calculated and, when possible, the function ...
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