Single cell sequencing
The single-cell sequencing: new developments and medical applications
... animals, single-cell transcriptome sequenc- ing technology is used to map the cell development of zebrafish and African cockroach embryos, providing important clues for understanding developmental ...
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Understanding tumor ecosystems by single cell sequencing: promises and limitations
... with single-cell sequencing have made meaningful progress in this ...of single cells or important ‘anchor genes’ via FISH, smFISH, immunohistochemis- try, laser capture microdissection, laser ...
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OncoNEM: inferring tumor evolution from single cell sequencing data
... recurrent sequencing errors ...yielding single nucleotide variant (SNV) data sets with large fractions of false negatives ...in single-cell sequencing data sets ...
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Single cell sequencing reveals karyotype heterogeneity in murine and human malignancies
... of single-cell sequencing were isolated from thymuses by dissecting the organ and mincing the tissue through a 100 μm cell ...a single well to serve as a positive control, as well as an ...
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Future medical applications of single-cell sequencing in cancer
... of single-cell sequencing will be in the genomic profiling of copy number or sequence mutations in CTCs and disseminated tumor cells (DTCs) (Figure ...genome sequencing of single CTCs ...
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High-resolution single-cell sequencing of malaria parasites.
... analysis. Single-cell sequencing of ...Fluorescence-Activated Cell Sorting and whole genome ...48 single-cell genomes derived from a polyclonal infection sampled in Chikhwawa, ...
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Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
... clear cell renal cell carcinoma; CD45−: leukocyte common antigen 45−; CDC27: cell division cycle 27; cDNA: complimentary DNA; CEL‑seq: cell expression by linear ampli‑ fication and ...
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Single cell sequencing in stem cell biology
... omic sequencing field has already had such great influence and has contributed so tremendously to numerous biological ...current single-cell omic se- quencing technologies, all of which are based on ...
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Holo Seq: single cell sequencing of holo transcriptome
... mRNA sequencing li- brary following the manufacturer’s protocol after poly-A selection (NEB 7530; Additional file 1: Figure ...the sequencing library, we digested the library with Not I before ...
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Single cell sequencing: a distinct new field
... from single cell RNA, leading to the detection of alternatively spliced exons ...Similarly, single cell tagged reverse-transcription (STRT) is based on the template-switching property of the ...
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PBMC fixation and processing for Chromium single cell RNA sequencing
... primary cell types, such as blood monocytes, rapidly undergo changes once isolated from whole ...stop cell stress/perturbation during the experi- ...of cell types is essential for disconnecting time ...
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Design and computational analysis of single cell RNA sequencing experiments
... in single-cell sequencing technologies and con- comitant advances in computational and statistical ...from single-cell RNA-seq, as well as other types of single-cell ...of ...
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Extraction of total RNA from single oocytes and single cell mRNA sequencing of swine oocytes
... generation sequencing, single-oocyte RNA sequencing data have been generated for mice [4–6], cattle [7], goats [8], and humans ...of single oocytes are essen- tial for a fine dissection of ...
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Current Progresses of Single Cell DNA Sequencing in Breast Cancer Research
... high-throughput sequencing data is a very complicated and time-consuming ...bulk sequencing and single cell sequencing follow a similar pipeline as briefly summarized ...of ...
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Single cell RNA sequencing to dissect the molecular heterogeneity in lupus nephritis
... by cell sorting, it was important to demonstrate the ability to differen- tiate cell types on transcriptomic data ...expected cell types, a limitation of the study was the inability to identify ...
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Going the Distance: Optimizing RNA-Seq Strategies for Transcriptomic Analysis of Complex Viral Genomes
... In this Gem, we highlight the major challenges that can arise when studying viral transcriptomes with the leading RNA-Seq technologies. The limitations are most evi- dent for viruses with large gene-dense double-stranded ...
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Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity
... SMRT Cell of PacBio ...PacBio sequencing was not proportional to their abundance in the starting ...the cell barcodes and UMIs in conjunction with the standard 10x Illumina sequencing to ...
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Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
... A. Dilution experiment summary. See section 3.5.1 for detailed information. B. Single cell amplification methods used. Protocols involve two key steps: conversion of RNA (blue) to cDNA (green), and ...
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Single-Cell Epigenomics In Cancer Research
... bisulfite sequencing, both GpC sites (methylated in accessible chromatin regions by MTase) and endogenous CpG methylation sites can be simultaneously analysed, allowing profiling of chromatin accessibility and ...
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UMI count modeling and differential expression analysis for single cell RNA sequencing
... different cell popula- tions and tissues ...of single-cell isolation, cell lysis, tran- script capture, complementary DNA (cDNA) conversion and amplification, library preparation, and ...
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