Single-Chain Antibodies
Isolation of High-Affinity Single-Chain Antibodies against Mycobacterium avium subsp. paratuberculosis Surface Proteins from Sheep with Johne's Disease
... paratuberculosis antibodies to be used as a separation tool to detect ...SurfS2.2 single-chain antibodies can bind to and selectively isolate target ...polyclonal antibodies may be very ...
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Rey, Clémence (2006): Single chain antibodies against the 37 kDa/67 kDa laminin receptor as tools for prion diseases therapy. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... specific antibodies as powerful therapeutic/prophylactic ...polyclonal antibodies are inappropriate for therapeutic applications, we developed monoclonal single chain antibodies (scFv) ...
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Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for Cancer-Targeted Therapy
... monoclonal antibodies, some important problems have been reported; these include high production cost, low tissue penetration, and the human anti-mouse antibody response (HAMA ...synthesized antibodies are ...
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Cell-Type-Specific Gene Transfer into Human Cells with Retroviral Vectors That Display Single-Chain Antibodies
... displayed single-chain antibodies (scAs) directed against a carcinoembryonic antigen-cross-reacting cell surface protein, we have shown that an efficient, cell-type-specific gene delivery can be ...
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The expression and metal affinity purification of single-chain antibodies
... As a purification process, the losses are generally higher than those obtained using mucin-affinity chromatography, with the exception of imidazole elution methods. Imidazole elutions provide high yields but poor ...
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Toward highly efficient cell-type-specific gene transfer with retroviral vectors displaying single-chain antibodies.
... different antibodies, one directed against a cell surface protein and the other directed against the viral envelope (Env) protein, were linked by streptavidin at their carboxy termini to direct a retroviral vector ...
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Selection and Evaluation of Specific Single Chain Antibodies against CD90, a Marker for Mesenchymal and Cancer Stem Cells
... These antibodies are small and effective and can be easily expressed in functional form in Escherichia coli ...these antibodies allows for more rapid and even penetration to and into tumors and other ...
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Amann, Maria (2009): Anti-tumor activity, pharmacology and toxicology of EpCAM/ CD3-bispecific single-chain antibodies. Dissertation, LMU München: Fakultät für Biologie
... Offner et al. showed that the BiTE-induced cytolytic immunological synapse was indistin- guishable in composition, sub-domain arrangement and size from regular MHC I-peptide (erbB2) induced synapses and was formed even ...
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Isolation of Neutralizing Human Single Chain Antibodies Against Conserved Hemagglutinin Epitopes of Influenza a Virus H3N2 Strain
... enables the virus to be released from the host cell and is required for influenza virus replication (5). The influenza A genome contains eight gene segments of single-stranded, negative-sense RNA that encodes for ...
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Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... produce single-chain antibodies (scFv) against Clostridium difficile toxin ...Recombinant single-chain antibodies were expressed in Escherichia ...the single-chain ...
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Analysis of the relationship between end-to-end distance and activity of single-chain antibody against colorectal carcinoma
... bivalent single-chain antibodies targeting colorectal cancer with linkers of 5-15 amino acids ...bivalent single-chain antibody's affinity and ...
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Stop-Frame Filming and Discovery of Reactions at the Single-Molecule Level by Transmission Electron Microscopy
... sufficient single-molecule contrast and, most importantly, the molecule must be unreactive until sufficient energy is delivered from the e-beam to break the bonds between the atoms and initiate ...a single ...
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Effector Functions of Camelid Heavy-Chain Antibodies in Immunity to West Nile Virus
... Clearance of pathogens by antibodies is initiated when an antigenic site is bound by the CDRs of the immunoglobulin V domain. Binding may neutralize viruses, toxins, or enzymes deployed by the pathogen. While ...
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Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... The aim of this study was to obtain the recom- binant murine single-chain variable fragment anti- body (scFv) to carboxyterminal end of human pro- tein C light chain. ScFv-antibodies could be ...
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Loose fit polypseudorotaxanes constructed from γ CDS and PHEMA PPG PEG PPG PHEMA
... Cyclodextrins (CDs) are a family of cyclic oligosaccharides composed of 6, 7 or 8 glucose units linked via α-1,4-glycosidic bonds. Due to the presence of a hydrophobic inner cavity with different geometric dimensions, ...
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Isolation and characterization of camelid single domain antibodies against HER2
... We immunized a male llama (Lama glama) with recombinant human HER2 ectodomain (Cat. No. HE2- H5225; ACROBiosystems, Beijing, China) as previously described [5–7]. Briefly, the animal was immunized sub- cutaneously five ...
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Fungal Lipids: The Biochemistry of Lipid Accumulation - Volume 5 Number 5 (Oct. 2014) - IJCEA
... in single cell oils (SCOs) accumulated by oleaginous fungi centers around the ability of these microorganisms to convert agro-industrial surpluses and residues into lipids as potential alternative to edible plant ...
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Have you ever seen a phonon?
... In both these cases, the periodicity is broken, Bloch’s theorem no longer applies, and there are no analytic solutions to compare with. Of course, at the same time the wave numbers are no longer good quantum numbers, but ...
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Hematologic Indices, Inflammatory Markers, Symptoms and Routine Diagnostic Tests in Childhood Brucellosis
... polymerase chain reaction (PCR), and serologic tests which detect anti-Brucella antibodies, the most accepted of them being tube Wright agglutination ...
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Stop-Frame Filming and Discovery of Reactions at the Single-Molecule Level by Transmission Electron Microscopy
... to provide an alternative way to study the mechanisms of chemical reactions. The combination of sub-angstrom spatial resolu- tion with a larger overall fi eld of view and an image capture rate signi fi cantly faster than ...
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