Single-chain antibody variable fragment
Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
... MDCK cells were washed 3 times with PBS and fixed in cold fixative solution (acetone: methanol, 3:1) for 10 min at 4°C followed by 3 washes with PBS and incubation for 1 h at 37°C with individual primary antibodies. The ...
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Synthesis of a soluble flag tagged single chain variable fragment (scFv) antibody targeting Cucumber Mosaic Virus (CMV) coat protein
... and transformed E. coli DH5 α (supE44, hsdR17, recA1, endA1, gyrA96, thi-1, relA1) with the construct. T7 (5'-TAA TAG ACG ACT CAC TAT AGG G-3') and SP6 (5'-CTA TTT AGG TGA CAC TAT AG-3') primers were used for PCR ...
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Cloning approach and functional analysis of anti intimin single chain variable fragment (scFv)
... One milliliter of the growth culture was centrifuged at 12,000 × g for 10 min and washed three times in PBS buffer. The pellet was permeabilized in 4% Triton X-100 under constant shaking (200 rpm) for 10 min and washed ...
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<p>Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis</p>
... The selected clone groups were divided into four groups: Gr-1, Gr-2, Gr-3, and Gr-4 (Figure S1). The sequence encod- ing VasSF was subcloned to optimize expression in E. coli. One Gr-1 clone (subclones Rq01, G1LQq, G1Qq, ...
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Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein
... The variable regions of the heavy and light chains (VH and VL, respectively) of an antibody are essential for the recog- nition of ...antigens. Single-chain variable-region fragments ...
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Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris
... bodies [29], scFv-Fc [30], scFv-immunotoxins [31,32] and full-length IgG [33,34]. Moreover, favorable protein folding by P. pastoris seems to play a fundamental role in the stability and activity of a single ...
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Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes
... are antibody moieties, and especially engineered antibody fragments [13] such as single chain variable fragment (scFv) [10], disulfide-stabilized Fv antibody ...
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A novel placental like alkaline phosphate promoter driven transcriptional silencing combined with single chain variable fragment antibody based virosomal delivery for neoplastic cell targeting
... Background: Placental like alkaline phosphate (PLAP), an oncofetal antigen, is highly expressed in germ cell, cervical, ovarian and several other tumour types but minimally in normal tissues. The expression of a PLAP ...
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The Antiviral Mechanism of an Influenza A Virus Nucleoprotein Specific Single Domain Antibody Fragment
... expressed variable domains of alpaca heavy-chain-only antibodies (VHHs) that target IAV ...a single variable binding ...~15-kDa antibody domains can bind with high affinity and spec- ...
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Periplasmic secretion, extraction and purification of recombinant tagless anti tumor necrosis factor (TNF) alpha single chain variable fragment (scFv) in E coli
... alpha antibody, administered every four weeks as a subcutaneous injection in psoriatic arthritis: Twenty-four- week efficacy and safety results of a randomized, placebo- controlled ...
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Adenovirus-mediated anti-AEG-1 ScFv expression driven by stathmin promoter inhibits tumor growth in cervical cancer
... Specific single-chain variable antibody fragment (ScFv) is one kind of minisize ...by variable heavy chain and light chain domains with a polypeptide linker, which ...
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Single Chain Fragment Variables Antibody binding to EGF Receptor in the Surface of MCF7 Breast Cancer Cell Line: Application and Production Review
... ScFv antibody is neutralising antibodies, which, they can connect with toxins and viruses and terminate them with high ...rabies-neutralising single chain fragment variable ...
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Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... primary antibody of mouse anti-myc antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was added with the ratio of 1 to ...secondary antibody of the HRP-conjugated anti- mouse IgG (Santa Cruz ...
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Enhanced Bovine Herpesvirus Type 1 Neutralization by Multimerized Single-Chain Variable Antibody Fragments Regardless of Differential Glycosylation
... their variable-region fragments (7) makes these proteins suitable for clinical diagnosis, immunodiag- nostics, and ...A single-chain variable fragment (scFv) is an example of a ...
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The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis
... a single-chain variable fragment antibody against the green fluorescent protein (GFP- specific scFv) protein was used ...The variable frag- ment encoding DNA-sequence derived ...
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Expression of a Functional Single-Chain Variable-Fragment Antibody against Complement Receptor 1 in Streptococcus gordonii
... the antibody to bind to the Fc ␥ III/II receptors to eliminate background binding in subsequent ...(1/200) antibody was added to the first three aliquots, and PBS-BSA buffer was added to the fourth ...
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... Phage display antibodies offer a way to produce high-affinity single-chain variable fragment (scFv) derivatives of human an- tibodies of “natural host” origin (8). Our goal was to produce ...
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Current applications of antibody microarrays
... scFvs: single‑chain variable fragments; Fab: fragment antigen‑binding; CD: cluster of differentiation; ELISA: enzyme‑linked immunosorbent assays; DNA: deoxyribonucleic acid; MS: mass ...
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Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... Therefore, the recombinant scFv antibodies to important component of anticoagulation blood system protein C were obtained. It was shown that the selected antibodies could be used in enzyme- linked immunosorbent assay and ...
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Extracellular vesicles expressing a single-chain variable fragment of an HIV-1 specific antibody selectively target Env+ tissues
... Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, depending on the size of the structures, are nano-sized, membrane-enveloped structures containing mostly proteins and nucleic acids ...
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