Single Chain Fragment Variable
<p>Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis</p>
... Background: Anti-neutrophil cytoplasmic autoantibodies (ANCA) associated vasculitis is a pauci-immune disease with the inflammation of the small blood vessels. The efficacies of antibody drugs for induction therapies of ...
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Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris
... bodies [29], scFv-Fc [30], scFv-immunotoxins [31,32] and full-length IgG [33,34]. Moreover, favorable protein folding by P. pastoris seems to play a fundamental role in the stability and activity of a single ...
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Correctness and accuracy of template based modeled single chain fragment variable (scFv) protein anti breast cancer cell line (MCF 7)
... Figure 3a shows several amino acid variations and insertion regions, especially between the heavy-chain amino acid sequence alignment with the 3BKY template and as shows in Figure 3b the[r] ...
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Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... The aim of this study was to obtain the recom- binant murine single-chain variable fragment anti- body (scFv) to carboxyterminal end of human pro- tein C light chain. ScFv-antibodies ...
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Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... our single-chain antibodies as the capture antibody in the ELISA procedure, although under these conditions the small scFv molecule can undergo partial denaturation ...of single-chain antibody ...
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Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes
... as single chain variable fragment (scFv) [10], disulfide-stabilized Fv antibody fragment (ds-Fv), ds-scFv, single chain antibodies (sdAb) and ...antibody fragment ...
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Synthesis of a soluble flag tagged single chain variable fragment (scFv) antibody targeting Cucumber Mosaic Virus (CMV) coat protein
... A single chain variable fragment (scFv) antibody, targeted to CMV coat protein, was constructed with mRNA from the spleen of a CMV coat protein-immunized ...the variable heavy (V H ) ...
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Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
... addition, single chain variable fragments (scFvs) contain the complete antigen binding site, and specific scFvs can bind to and neutralize ...
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Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for Cancer-Targeted Therapy
... of single-chain antibodies (scFvs) compared to monoclonal antibodies in cancer immunotherapy, specific anti-CTLA-4 scFvs (single-chain variable fragment) were selected in this ...
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Current applications of antibody microarrays
... scFvs: single‑chain variable fragments; Fab: fragment antigen‑binding; CD: cluster of differentiation; ELISA: enzyme‑linked immunosorbent assays; DNA: deoxyribonucleic acid; MS: mass ...
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... cytes by other Plasmodium species, such as P. falciparum. The erythrocyte-binding capabilities of several proteins have been examined, including merozoite surface protein 1, reticulocyte binding protein, and DBP, which ...
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A novel placental like alkaline phosphate promoter driven transcriptional silencing combined with single chain variable fragment antibody based virosomal delivery for neoplastic cell targeting
... a single chain variable fragment antibody (scFv) based chimeric F-virosomal delivery system based on PAP, which is antigenically indistinguishable from PLAP ...
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Display of single-chain variable fragments on bacteriophage MS2 virus-like particles
... molecules. Single-chain variable fragments (scFvs) are modified ver- sions of full antibodies that contain the antigen binding site, but they are also more amenable to genetic manipu- lation than the ...
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Adenovirus-mediated anti-AEG-1 ScFv expression driven by stathmin promoter inhibits tumor growth in cervical cancer
... Specific single-chain variable antibody fragment (ScFv) is one kind of minisize ...by variable heavy chain and light chain domains with a polypeptide linker, which ...
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Gene therapy for carcinoma of the breast: Genetic ablation strategies
... anti-ErbB-2 single-chain variable fragment antibody (sFv), we were able to demonstrate downregulation of cell surface ErbB-2 and a corresponding specific growth inhibi- tion of cells ...
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Extracellular vesicles expressing a single-chain variable fragment of an HIV-1 specific antibody selectively target Env+ tissues
... a single chain variable fragment (scFv) of a high affinity HIV-1 Env-specific monoclonal antibody 10E8 to deliver chemotherapeutic drug curcumin (Cur) or apoptosis-inducing miR-143 to the ...
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Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... The results of fluorescence microscopy demonstrated that the isolated scFv was linked to the KDR3 cell surface, indicating the proper functioning of the scFv. The fluorescence signal in these cells was slightly lower ...
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Cloning approach and functional analysis of anti intimin single chain variable fragment (scFv)
... an EPEC isolate. Subsequent tests underway in our labora- tory could demonstrate if the scFv reacts with the different types of intimin expressed by EPEC and EHEC. These data will be extremely important, since the ...
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Periplasmic secretion, extraction and purification of recombinant tagless anti tumor necrosis factor (TNF) alpha single chain variable fragment (scFv) in E coli
... scFv fragments consist of variable domains of the heavy and light chains linked by a flexible linker. They were described as small fragments capable of retaining the binding activity of the full IgG molecule, ...
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Expression of a Functional Single-Chain Variable-Fragment Antibody against Complement Receptor 1 in Streptococcus gordonii
... Streptococcus gordonii, an oral commensal organism, is a candidate vector for oral-vaccine development. Previous studies have shown that recombinant S. gordonii expressing heterologous antigens was weakly immunogenic ...
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