Single-chain recombinant protein
Construction, expression and binding specificity of bispecific CD3 × VEGFR 2 and CD3 × NCAM antibodies in the single chain and diabody format
... between protein modifications present only on tumor cells but not on differentiated cells that express the same protein, by cost-effective production, antibody stability in the human circulation and by ...
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... molecules. Recombinant PvRII (C4-to-C7 cysteine-rich region of PvDBP II) protein of SK-1 strain isolated in South Korea was produced using a previously reported method ...of recombinant PvRII was ...
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<p>Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis</p>
... a single-chain antibody specific for activated ...fusion protein as a potential immunosuppressive agent for organ ...binds recombinant transmem- brane TNF-alpha and activates immune effector ...
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Characterization of a shorter recombinant polypeptide chain of bone morphogenetic protein 2 on osteoblast behaviour
... Reagent, Roche Applied Science, Germany) according to the manufacturer’s instructions. The concentration and quality of the total RNA samples was analyzed by Nanodrop (Thermo Fisher Scientific Inc.). Com- plementary DNA ...
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Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... our single-chain antibodies as the capture antibody in the ELISA procedure, although under these conditions the small scFv molecule can undergo partial denaturation ...of single-chain antibody ...
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Periplasmic secretion, extraction and purification of recombinant tagless anti tumor necrosis factor (TNF) alpha single chain variable fragment (scFv) in E coli
... Based on the specific interaction of immobilized metal ion on the chromatographic matrix and the protein of interest, IMAC was used to purify the protein. The epoxy groups present in the matrix were coupled ...
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Single-Chain Antibody Displayed on a Recombinant Measles Virus Confers Entry through the Tumor-Associated Carcinoembryonic Antigen
... superfamily protein class coupled with the dem- onstration that similar display of EGF and IGF allows fusion through the cognate tyrosine kinase receptors (28) is consistent with a rather flexible receptor usage ...
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Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory
... All genetic constructs were designed to create precise amino-terminal translational fusions of the scFv-coding sequences with the E. carotovora pelB signal sequence to target the scFvs for secretion by the Sec pathway. ...
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Differentiation of Candida albicans and Candida dubliniensis by Using Recombinant Human Antibody Single Chain Variable Fragments Specific for Hyphae
... light chain variable regions was used to select phage clones capable of binding to the surfaces of viable ...identified. Single-chain antibody variable fragments (scFv) derived from two of these ...
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Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... sequence of protein C, was performed in the Labora- tory of Peptide Immunogens of the State Scientific Centre Institute of Immunology FMBA. According to results of mass-spectroscopy and analytical HPLC the ...
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Structural and kinetic comparison of recombinant human single and two chain tissue plasminogen activator
... state protein fluorescence were also noted with denaturation of these plasminogen activators, as well as in the quenching of intrinsic fluorescence of the reduced, alkylated species by ...the single free ...
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Engineered recombinant protein products of the avian paramyxovirus type 1 nucleocapsid and phosphoprotein genes for serological diagnosis
... Currently, serological testing for avian avulavirus anti- bodies, in particular of APMV-1, as employed, e.g., for surveillance purposes, is traditionally based on HI assay or indirect ELISAs using whole virus antigen [2] ...
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Retargeting of Adenovirus Vectors through Genetic Fusion of a Single-Chain or Single-Domain Antibody to Capsid Protein IX
... a protein that was incorporated into ...this protein was efficiently incorporated into the Ad capsid ...capsid protein content showed that pIX-GFP was efficiently incorporated into the Ad capsid ...
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Inexpensive Designer Antigen for Anti-HIV Antibody Detection with High Sensitivity and Specificity
... multiple recombinant proteins for reliable di- agnosis of HIV infections adds to the cost of these EIA ...a single recombinant multiepitope protein (MEP) antigen, consisting of several ...
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Biosynthesis, purification, and characterization of the human coronavirus 229E 3C-like proteinase.
... activity albeit, in the case of N3029P, with a significant reduc- tion. Second, we replaced Glu-3074 with His. Glu-3074 is the only acidic amino acid between the catalytic His-3006 and Cys-3109 residues that is strictly ...
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Production of a Candidate Recombinant Protein Vaccine for Mannheimia haemolytica in Lettuce and Tobacco Chloroplasts
... by protein post- translational modifications (explored in section ...this recombinant protein may be more useful than ...ER-targeted protein may be caused by protein modifications that ...
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Targeted tumor killing via an intracellular antibody against erbB 2
... a recombinant adenovirus encoding an anti-erbB-2 single chain antibody to achieve targeted tumor cell killing in vivo and can show significantly prolonged survival of animals carrying a human ovarian ...
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Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis
... the recombinant Baylisascaris procyonis RAG1 protein (rBpRAG1) was developed, and early testing showed no cross- reactivity with Toxocara infections ...the recombinant protein rBpRAG1 was ...
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Cloning, expression, and immunocharacterization of surface protein containing an altered thrombospondin repeat domain (SPATR) from Plasmodium knowlesi
... infection is fast spreading and potentially dangerous, which makes accurate diagnosis vital for effective treat- ment of this parasitic disease. However, P. knowlesi para- sites are often misdiagnosed as Plasmodium ...
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In vivo activity of plant based interleukin 12 in the lung of Balb/c mouse
... commercial recombinant, rIL-12). IL-12 protein level in lungs was higher in mice treated with IL-12 than that seen in control mice at all evaluated time points (Figure 1A), which indicates that IL-12 was ...
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