Single chain variable fragment antibody (scFv)
A novel placental like alkaline phosphate promoter driven transcriptional silencing combined with single chain variable fragment antibody based virosomal delivery for neoplastic cell targeting
... a single chain variable fragment antibody (scFv) based chimeric F-virosomal delivery system based on PAP, which is antigenically indistinguishable from PLAP ...
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Expression of a Functional Single-Chain Variable-Fragment Antibody against Complement Receptor 1 in Streptococcus gordonii
... Oral colonization. The oral colonization of 4-week-old BALB/c mice (female; n ⫽ 3) with S. gordonii SecCR1 and S. gordonii SL3 was carried out using methods described previously (16). S. gordonii SL3 is a ...
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Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... purified scFv binding affinity when comparing them with other antibodies, it was found that the given antibody had acceptable binding ...that scFv fragments have been used to the cell surface ...
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The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis
... a single-chain variable fragment antibody against the green fluorescent protein (GFP- specific scFv) protein was used ...The variable frag- ment encoding DNA-sequence ...
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Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... our single-chain antibodies as the capture antibody in the ELISA procedure, although under these conditions the small scFv molecule can undergo partial denaturation ...of ...
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Extracellular vesicles expressing a single-chain variable fragment of an HIV-1 specific antibody selectively target Env+ tissues
... Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, depending on the size of the structures, are nano-sized, membrane-enveloped structures containing mostly proteins and nucleic acids ...
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Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
... addition, single chain variable fragments (scFvs) contain the complete antigen binding site, and specific scFvs can bind to and neutralize ...with scFv or siRNA-NP604 ...
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Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains
... simpler antibody fragments with full antigen bind- ing capacity have been developed for research purposes, where biological activity is more important than structural authenticity and correct glycosylation ...
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... cytes by other Plasmodium species, such as P. falciparum. The erythrocyte-binding capabilities of several proteins have been examined, including merozoite surface protein 1, reticulocyte binding protein, and DBP, which ...
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<p>Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis</p>
... of antibody drugs for induction therapies of vasculitis vary among ...a single chain Fv region (ScFv) with vasculitis-specific therapeutic ...human ScFv based on the therapeutic ...
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Periplasmic secretion, extraction and purification of recombinant tagless anti tumor necrosis factor (TNF) alpha single chain variable fragment (scFv) in E coli
... Plasmid, E. coli strains, antigen and antibody, pET-20b(+) vector (Novagen-Cat. # 69739-3) used as the expression vector as it carries an N-terminal pelB signal sequence for periplasmic localization. E. coli ...
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Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris
... [29], scFv-Fc [30], scFv-immunotoxins [31,32] and full-length IgG ...a single domain antibody frag- ment against botulinum neurotoxin in comparison to the same produced in ...
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Gene therapy for carcinoma of the breast: Genetic ablation strategies
... anti-ErbB-2 single-chain variable fragment antibody (sFv), we were able to demonstrate downregulation of cell surface ErbB-2 and a corresponding specific growth inhibi- tion of cells ...
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Synthesis of a soluble flag tagged single chain variable fragment (scFv) antibody targeting Cucumber Mosaic Virus (CMV) coat protein
... A single chain variable fragment (scFv) antibody, targeted to CMV coat protein, was constructed with mRNA from the spleen of a CMV coat protein-immunized ...the variable ...
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Cloning approach and functional analysis of anti intimin single chain variable fragment (scFv)
... One milliliter of the growth culture was centrifuged at 12,000 × g for 10 min and washed three times in PBS buffer. The pellet was permeabilized in 4% Triton X-100 under constant shaking (200 rpm) for 10 min and washed ...
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Adenovirus-mediated anti-AEG-1 ScFv expression driven by stathmin promoter inhibits tumor growth in cervical cancer
... Specific single-chain variable antibody fragment (ScFv) is one kind of minisize ...by variable heavy chain and light chain domains with a polypeptide linker, ...
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Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... of scFv 9E was inserted into the pET-22b vector by the NcoI/NotI sites ...for scFv 9E molecular weight (about 30 kDа) were found ...the scFv 9E production was considerably intensified. Expectedly, ...
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The Antiviral Mechanism of an Influenza A Virus Nucleoprotein Specific Single Domain Antibody Fragment
... expressed variable domains of alpaca heavy-chain-only antibodies (VHHs) that target IAV ...a single variable binding ...~15-kDa antibody domains can bind with high affinity and spec- ...
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Platelet-Targeted Delivery of Peripheral Blood Mononuclear Cells to the Ischemic Heart Restores Cardiac Function after Ischemia-Reperfusion Injury
... therefore their function may be driving the paracrine-mediated cardiac-repair mechanisms when delivered immediately after AMI. While the roles of monocytes and macrophages are well characterized, there is little known ...
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Targeting CEA in Pancreas Cancer Xenografts with a Mutated scFv-Fc Antibody Fragment
... anti-CEA scFv-Fc H310A antibody frag- ment ...our scFv-Fc (H310A) antibody ...anti-CEA scFv-Fc (H310A) antibody fragment for in vivo antigen-specific tumor targeting of ...
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