Single copy
The chordate ancestor possessed a single copy of the Brachyury gene for notochord acquisition
... new copy with a secondary function, and that the duplication occurred very early in chordate ...a single copy in the common ancestor of chordates and a single copy of Brachyury acquired ...
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Improved Single Copy Assays for Quantification of Persistent HIV 1 Viremia in Patients on Suppressive Antiretroviral Therapy
... In summary, we have developed and validated an improved single-copy assay (iSCA) with an improved ability to detect and quantify low levels of plasma viremia. iSCA will undoubtedly be useful for future ...
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The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
... from single flow‑sorted chromosomes as ...any single‑copy sequence not only in wheat, but in any plant species where suspension of intact mitotic chromosomes suitable for flow cytometric sorting can ...
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A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome
... could place a heavy metabolic burden on the host bac- terium. In order to circumvent these and other pro- blems, we previously developed S. aureus vectors that allow cloning of genes in single copy [2,3]. ...
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Single-copy nuclear genes resolve the phylogeny of the holometabolous insects
... a single locus for phylogenetic res- olution, though useful in some circumstances, can clearly result in inaccurate ...No single gene in our dataset recovers our well-supported phylogeny that is con- gruent ...
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Deletion of a single-copy tRNA affects microtubule function in Saccharomyces cerevisiae.
... rtsl-1 mutation represents an alteration at a single genetic locus that confers suppression of the cold- sensitive tub2-104 allele and heat sensitivity.. When the rtsl-[r] ...
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Can too many copies spoil the broth?
... increasing copy number did not increase titer when using the GAP promoter, but when using the AOX1 promoter, titer increased from a single copy clone to two copies and then reduced dra- matically (to ...
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The cHS4 Insulator Increases the Probability of Retroviral Expression at Random Chromosomal Integration Sites
... a single copy of the different recombinant retroviruses, we observed that the cHS4 insulator increases the probability of expression at ran- dom integration sites from 7 of 34 (or 21%) to 25 of 34 (or 74%) ...
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DNA SEQUENCE COMPARISON AMONG CLOSELY RELATED DROSOPHILA SPECIES IN THE MULLERI COMPLEX
... We examined the extent of hybridization and the thermal stability of both single-copy and middle-repetitive DNA sequences from five Drosophila species in the muller[r] ...
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A Novel PCR Assay for Quantification of HIV-1 RNA
... a single-copy assay (SCA) that has become the definitive assay for residual viremia in patients on HAART whose plasma HIV-1 RNA levels are below the limit of detec- tion of the clinical assay (10, ...the ...
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Chromatin Structure and Differential Accessibility of Homologous Human Mitotic Metaphase Chromosomes
... by single copy DNA FISH probes ...DA. Single copy FISH probes detecting no DA ...for single copy FISH as described previously (15,16). Single copy probes detecting ...
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NONIDEAL STATISTICS AND POSITIVE CORRELATION IN PHAGE RECOMBINATION: STUDIES WITH Λ TANDEM DUPLICATION PHAGES
... Second, comparison of the frequency of second recombinations among the triplication (and single-copy) products of like-parent recombination with that among unselected [r] ...
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Loss of heterozygosity: what is it good for?
... Haplo-insufficiency: Single copy gene ...(particularly copy-number neutral LOH) are enriched for deleterious mutations and increased promoter methylation, while copy-number loss LOH regions ...
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GENE ACTIVITY DURING DEVELOPMENT OF THE CELLULAR SLIME MOLD DICTYOSTELIUM DISCOIDEUM
... mRNA molecules contain short and repetitive sequences associated with them and exclude the notion that 90% of the molecules are transcribed solely from single-copy sequence[r] ...
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Distinct Roles of Candida albicans-Specific Genes in Host-Pathogen Interactions
... a single copy of PGA16 at the RP10 locus was sufficient to increase virulence in the mouse model of disseminated candidiasis but did not increase pathoge- nicity in an in vitro epithelial infection ...
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Characterization of Hortulanus Endogenous Murine Leukemia Virus, an Endogenous Provirus That Encodes an Infectious Murine Leukemia Virus of a Novel Subgroup
... a single copy in the genome of Mus spicilegus, was remarkable for characteristics that suggested that it was ancient and related to the hypothetical common ancestor of murine leukemia viruses (MLVs) and ...
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Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris
... the higher production of the isolated strains (Fig. 2). The yield of the strains with the reference construct increases from 1.0 (single copy) to 7.9 (the best strain, 2.0 mg/mL zeocin), while for the UNall ...
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Rates of DNA evolution in Drosophila depend on function and developmental stage of expression.
... The results indicate: (1) cDNA is less diverged than total single-copy DNA; (2) cDNA sequences are not in the rapidly evolving fraction of the single-copy genome of Drosop[r] ...
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Comparison of the urinary excretion of quercetin glycosides from red onion and aglycone from dietary supplements in healthy subjects: A randomized, single-blinded, cross-over study.
... Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single ...
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Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
... increased copy number. The resulting strains Fig. 5 Single copy starting clones result in higher copy number ...final copy numbers after plate PTVA and 24 h liquid PTVA (L24) for two ...
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