Single Molecule Fluorescence Microscopy
Towards Single-molecule Fluorescence Microscopy on a Mobile Phone Using Plasmonic Nanohole.
... of single-molecule microscopy dates back to the pioneering work from Moerner and Kador, who used absorption to detect dye molecules embedded in a crystalline matrix at cryogenic ...years, ...
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Dynamics of DNA Mismatch Repair Initiation Complexes Revealed by Single Molecule Fluorescence.
... In the liposome encapsulation experiments, we used 70nM MutS, which is expected to give a higher tetramer fraction than at 10 nM. We repeated two encapsulation type experiments for donor/acceptor labeled MutS at 7 fold ...
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Vogelsang, Jan (2009): Advancing single-molecule fluorescence spectroscopy and super-resolution microscopy with organic fluorophores. Dissertation, LMU München: Fakultät für Physik
... in fluorescence spectroscopy and microscopy this has long appeared to be a necessary ...modern fluorescence-based ...of fluorescence microscopy and different ...
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Ensemble and single-molecule fluorescence studies of DNA mismatch repair initiation by MutS
... force microscopy uncovered a second, unbent DNA conformational state induced by MutS at a mismatch, suggesting that there may be an equilibrium between bending and unbending that is essential for mismatch repair ...
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Orthogonal Probing of Single-Molecule Heterogeneity by Correlative Fluorescence and Force Microscopy
... bend angle distribution was fi t using the sum of two folded Gaussians, where the fi rst population (64% of all complexes) represents the state wherein EcoRV does not impose a protein- induced bend angle, and the second ...
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Frontiers in fluorescence microscopy
... a single point mutation (Thr 203 _His) in avGFP (Patterson and Lippincott- Schwartz, ...no fluorescence emission when excited at 480 – 510 ...green fluorescence (excitation: 504 nm; emission: 517 ...
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Medicinal value of asiaticoside for Alzheimer’s disease as assessed using single molecule detection fluorescence correlation spectroscopy, laser scanning microscopy, transmission electron microscopy, and in silico docking
... form a beta strand–turn–beta strand motif that con- tains two parallel, in-register beta sheets that are formed by residues 18–26 (β1) and 31–42 (β2). Inter- molecular side chain contacts are formed between the ...
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Single Molecule investigations of DNA Looping Using the Tethered Particle Method and Translocation by Acto-Myosin Using Polarized Total Internal Reflection Fluorescence Microscopy
... The molecule “walks” to the right towards the “barbed” end of the actin filament, typically taking 36 nm steps so that binding occurs on an actin monomer that is favorably oriented on the pseudo-repeat of the ...
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Control of Single Molecule Fluorescence Dynamics by Stimulated Emission Depletion
... of single molecule fluorescence can be readily observed using continuous wave (CW) illumination ...a single-photon avalanched diode (SPAD, SPCM-AQR-14, EG&G) are ...
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Observation of Single-Molecule Rotational Diffusion at Microsecond Timescale by Polarized Fluorescence Correlation Spectroscopy
... stock at 950 ◦ C. 2.) SWNTs were ‘picked-up’ directly from the CVD substrate onto conventional silicon AFM cantilevers, and the same substrate was also used for the following three steps to minimize fabrication time. 3.) ...
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Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications
... fluorescence microscopy, fluorophores are considered as point sources of light, whose emitted photons are collected by the objective and eventually projected onto the detector unit (Figure ...a single GFP ...
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Superresolution fluorescence microscopy for 3D reconstruction of thick samples
... whole single-molecule ...the single-molecule spots was corrected as shown in Additional file 1: Figure ...immobilized single-molecules at varying z-positions with a 100 nm ...individual ...
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Single-molecule localization microscopy analysis with ImageJ
... localization microscopy (PALM/FPALM) [11, 12] and (direct) stochastic optical reconstruction microscopy (STORM/dSTORM) [13, 14], which are all based on separating single photoswitchable fluorophores ...
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Q&A: Single-molecule localization microscopy for biological imaging
... To photoactivate and excite fluorophores in the sample, it is necessary to add the appropriate laser lines to an existing microscope. The choice of the lasers used depends on the activation and excitation spectra of the ...
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Analytical tools for single-molecule fluorescence imaging in cellulo
... ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become ...commonplace. Single-molecule ...
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Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide
... and single-molecule detection of freely-diffusing ...Normalized fluorescence emission spectra of free Alexa488 SDP, Alexa488-labelled disaccharide 6 and Alexa488-labelled ...c) Fluorescence ...
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Combined single-molecule force and fluorescence measurements for biology
... some single-molecule experiments ...resolution single-molecule experiments, sug- gesting that quantum dots may become invaluable in the field of single-molecule probes ...a ...
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Light sheet fluorescence microscopy for neuroscience
... induced aberrations such that they closely approximate the cubic modulation necessary to form an Airy light sheet [96].Vettenburg et al. [77] have shown that Airy beams yield thinner light sheets over larger FOVs when ...
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Monitoring HIV-1 Assembly in Living Cells: Insight from Dynamic and Single Molecule Microscopy
... optical microscopy has overcome the old resolution barrier -the diffraction limit- enabling visualization of previously invisible molecular details in biological ...resolution microscopy (SRM) techniques ...
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Detecting the Conformation of Individual Proteins in Live Cells using Single Molecule Fluorescence Resonance Energy Transfer.
... The use of smFRET in vivo to determine individual protein conformation has not been achieved because of the inherent difficulty of measuring two-color signal from single molecules in the optically noisy ...
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