Single Molecule Real Time (SMRT™) DNA Sequencing
Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics
... parallel sequencing has emerged as a standard diagnostic tool in the medical set- ...read single molecule sequencers resolve these ...native DNA. The first commercially available long read ...
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Detecting DNA Base Modifications Using Single Molecule, Real-Time Sequencing
... bisulfite sequencing to convert unmethylated cytosine nucleotides to uracil nucleotides, and comparison of sequence reads from bisulfite-treated and untreated samples 2 .... SMRT sequencing, in ...
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Single molecule detection and DNA sequencing by synthesis
... visualize single GFP, most preceding studies used 2 to 8 kW/cm 2 illumination from ...of single GFP molecules within 10 to 100 msec ...observe single GFP ...the time constant for bleaching is ...
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The properties and applications of single molecule DNA sequencing
... RNA sequencing should also be possible with this system, but this has not been reported yet for natural RNA molecules because nucleotides bind repeatedly to the reverse transcriptase before nucleotide ...
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Phased diploid genome assembly with single-molecule real-time sequencing
... novo sequencing projects are shifting towards more heterogeneous samples, such as outbred, wild type diploid, polyploid non-model organisms, or highly rearranged disease samples including human ...
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Single-Molecule Real-Time (SMRT) Full-Length RNA-Sequencing Reveals Novel and Distinct mRNA Isoforms in Human Bone Marrow Cell Subpopulations.
... both time consuming and computationally costly with unsatisfactory ...Greater sequencing depth is one potential remedy to consider and appears to improve the detection of complex transcripts only in ...
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Mutation assay using single-molecule real-time (SMRTTM) sequencing technology
... Mutation assays capable of detecting somatic mutations at very low frequencies are important in the areas of environ- mental mutagenesis, carcinogenesis, epidemiology, and regulatory science. They are especially ...
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A nanofluidic device for real-time visualization of DNA-protein interactions on the single DNA molecule level
... Single DNA molecule techniques have revolutionized our understanding of DNA –protein ...the DNA molecule studied is attached to a bead or a ...of DNA molecules in nano ...
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Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
... bisulfite sequencing in combination with chemical or enzymatic conversion ...in SMRT sequencing, the kinetic signatures of 5mC, 5hmC, 5fC, and 5caC are different, there is the potential for direct ...
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Single Molecule Real Time Sequencing Combined with Optical Mapping Yields Completely Finished Fungal Genome
... Next-generation sequencing (NGS) technologies have increased the scalability, speed, and resolution of genomic se- quencing and, thus, have revolutionized genomic ...genome sequencing initiatives typically ...
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Single-Molecule Real-Time Transcript Sequencing Facilitates Common Wheat Genome Annotation and Grain Transcriptome Research
... transcriptome sequencing for obtaining full-length transcript sequence information in plants Full-length transcript sequence information is very use- ful for both genome annotation and gene function stud- ies in ...
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Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies
... of DNA/cDNA material is required to optimally load each sequencing channel, although lower quantities can be used for less than optimal aligned read yields per ...manufacturer-supplied sequencing ...
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Resolving the complete genome of Kuenenia stuttgartiensis from a membrane bioreactor enrichment using Single-Molecule Real-Time sequencing
... PacBio sequencing in 2012 and 2014, respectively), we observed the emergence of a new Kuenenia strain that quickly established dominance, outcompeting the KUST strain that had been stable for many years 20 ...a ...
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Real-time pathogen surveillance systems using DNA sequencing
... in real-time and provide up to date data for integration by public health investigators rather than trying to find the information in a published ...surveillance sequencing is limited to food borne ...
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Real-Time DNA Sequencing Using Detection of Pyrophosphate Release
... 5), sequencing by mass spectrometry (6), and An approach for real-time DNA sequencing without single molecule detection ...of DNA polymerase corporation of each ...
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Single Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis
... fluorescent molecule in the field of view will emit, including non-specifically bound labeled nucleotides and other auto-fluorescent ...a single DNA ...labeled molecule within this region ...
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Advances in single molecule nucleic acid sequencing
... growing DNA strand and that the fluorophore was able to be efficiently cleaved using near-UV irradiation, allowing the continuous identification of the template ...a time is able to be incorporated, ...
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Nanoplumbing for a Single Molecule Study of DNA.
... for single molecule experiments of DNA-proteins interaction in real-time, specifically involving DNA and two ...lambda DNA in a ...in DNA extension exposed to ...
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Profiling infectious disease via single-cell and single-molecule sequencing
... cell-free DNA “Cell-free DNA (cfDNA) in biological fluids provides a rich window into human ...microbial DNA, as well as falsely-assigned taxa, thereby greatly improving the resolution of metagenomic ...
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Chapter 9 - Single Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis
... in single molecule sequencing by fluorescence is useful for reducing noise and false positive signals from unspecific binding of nucleotides, and is applicable in other situations where a tightly ...
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