single molecule sequencing DNA
Single molecule detection and DNA sequencing by synthesis
... To examine the PEM surface charge, we exposed positively and negatively terminated PEM surfaces to dATP-TMR, which carries effectively three negative charges per molecule at pH8.0. Thus we expected to detect much ...
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Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
... bisulfite sequencing in combination with chemical or enzymatic conversion ...SMRT sequencing, the kinetic signatures of 5mC, 5hmC, 5fC, and 5caC are different, there is the potential for direct ...
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Single molecule protein sequencing based on the superspecificity of tRNA synthetases
... Both DNA and RNA carry a uniform negative charge along their backbone, so they flow from cis to trans in Figure 1 ...of DNA, RNA, or protein through the pore causes a reduction in the pore current that ...
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Mutation assay using single-molecule real-time (SMRTTM) sequencing technology
... Mutation assays capable of detecting somatic mutations at very low frequencies are important in the areas of environ- mental mutagenesis, carcinogenesis, epidemiology, and regulatory science. They are especially ...
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Detection of low frequency <em>FGFR3</em> mutations in the urine of bladder cancer patients using next-generation deep sequencing
... with DNA markers due to the high abundance of normal DNA background present in the ...ultradeep sequencing technology to detect single mutant molecules of fibroblast growth factor receptor 3 ...
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Genome scaffolding and annotation for the pathogen vector Ixodes ricinus by ultra long single molecule sequencing
... high sequencing quality were blast-searched against proteins annotated in ...bind DNA, RNA or protein and to be involved in gene transcription, DNA replication, translational regulation, protein ...
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Resolving the Complexity of Human Skin Metagenomes Using Single Molecule Sequencing
... We demonstrated that long-read data, while lower in coverage and depth, can reconstruct community composition very simi- larly to short-read data. However, consistent with a recent report using synthetic long-read ...
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Single molecule sequencing reveals the molecular basis of multidrug resistance in ST772 methicillin resistant Staphylococcus aureus
... A sequencing library (SMRTbell) was generated from approximately 2 ug of genomic DNA sheared to an average fragment length of 10 ...using DNA Template Prep Kit 2.0 (3 – 10 Kb). The sequencing ...
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Covalent DNA Modifications in Phage and Bacterial Dynamics
... covalent DNA modifications that neutralize ...bacterial DNA and DNA from purified virus like particles (VLPs) were sequenced using Illumina and Pacific Bioscience single-molecule ...
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5935.pdf
... by sequencing represents a remarkably simple and generic approach for analysis of the global architectures of functionally important RNAs or ...or DNA strand is completely general and should inspire ...
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Sequencing of RAS/RAF pathway genes in primary colorectal cancer and matched liver and lung metastases
... a single molecule ...the DNA, followed by gapfilling through extension and ligation, resulting in a circular ...tification. Sequencing reads are aligned and reads shar- ing the same unique ...
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Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA
... the single-strand libraries contain higher complexity after the exome enrichment, namely 55–87% unique molecules, compared to previously reported double-strand library preparations of Illumina protocols with ...
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Exploiting single molecule transcript sequencing for eukaryotic gene prediction
... 800 μmol m −2 s −1 for 4 h prior to harvest. The heat treatment was performed by exposing the plants to a temperature of 35 °C for 3 h prior to harvest. All plants were harvested 30 days after germination, and the ...
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Advances in single molecule nucleic acid sequencing
... orders of magnitude less than current DNA sequencing methodology. Perhaps the most significant advantage of our SMS system is its unmatched level of parallelism. A 1″ × 1″ area is capable of holding ...
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Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies
... system’s single molecule nature and low input DNA/RNA requirements (400–1000 amol) are particularly advantageous for settings with limited cell/nucleic acid quantities (Goren et ...for DNA and ...
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Single Molecule Real Time Sequencing Combined with Optical Mapping Yields Completely Finished Fungal Genome
... few DNA transposon ...the DNA sequence (34, ...the DNA sequence and four retrotransposon families (VdLTRE8 to VdLTRE11) lacking ORFs (see Table S4 in the supplemental ...the DNA sequence are ...
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Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism
... Fig. 3 Graphical circular maps of T. brockianus GE-1 replicons. The complete genome of T. brockianus GE-1 is composed of a single circular chromosome that consists of 2,035,182 bp (a) and two circular plasmids, ...
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Ensemble and single-molecule fluorescence studies of DNA mismatch repair initiation by MutS
... fluorescent molecule in solution, where freely tumbling molecules exhibit a lower net anisotropy value than molecules interacting with larger binding molecules ...(ie. DNA bound by MutS). However, if the ...
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Binding dynamics of a monomeric SSB protein to DNA : a single molecule multi process approach
... and single-molecule data (Figures 4–8) indicate that two SsoSSB monomers can be ef- ficiently accommodated in a 12-mer ssDNA with nanomo- lar ...to DNA (62), no evidence for the formation of ...
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Recognition of base J in duplex DNA by J-binding protein
... to DNA Substrates Containing Multiple Js— During the titration experiment, using the Tel-4J substrate, we see an additional slower migrating species in the native gel in reactions containing higher concentrations ...
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