siRNA delivery
Strategies for ocular siRNA delivery: Potential and limitations of non-viral nanocarriers
... bind siRNA without the need for covalent attachment or encapsulation, and 2) the ability of amine containing cationic polymers to provide endosomal buffering and escape for intracytosolic siRNA ...acid ...
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Surface De-PEGylation Controls Nanoparticle-Mediated siRNA Delivery In Vitro and In Vivo
... NP-mediated siRNA delivery in vitro and in vivo. The siRNA NP platform (Figure 1a) was formulated by self-assembling a lipid-PEG layer on the surface of a poly(lactide-co-glycolide) (PLGA) polymer ...
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pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery
... intracellular siRNA delivery. Intracellular siRNA would regulate PLK1 expression and influence cell cycle, which resulted in a cell cycle arrest and apoptosis-inducing ...
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Comparing different siRNA delivery systems to target Diuraphis noxia
... three siRNA delivery techniques were compared, a novel siRNA delivery method developed and lastly, the methylation patterns in Dncprr1-8 were investigated as it has been reported that the ...
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Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer
... including siRNA condensation capacity, cell viability, and transfection ...transferring siRNA in ...with siRNA was analyzed by fluorescence imaging and immunofluorescent ...
10
Targeted siRNA Delivery Methods for RNAi-Based Therapies
... Topical delivery of siRNA is an attractive strategy to treat skin disorders that are resistant to drug treatment, including allergic contact dermatitis ...Topical siRNA has the potential to ...
214
Low molecular weight chitosan conjugated with folate for siRNA delivery in vitro: optimization studies
... Abstract: The low transfection efficiency of chitosan is one of its drawbacks as a gene delivery carrier. Low molecular weight chitosan may help to form small-sized polymer-DNA or small interfering RNA ...
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Development of a Cationic Mucic Acid Polymer-Based Nanoparticle siRNA Delivery System
... (mRNA). siRNA is a promising candidate as a therapeutic for silencing one or more overexpressed or mutated messenger RNA (mRNA) sequences encoding any type of protein that drives many types of malignant ...
261
ATP-activated decrosslinking and charge-reversal vectors for siRNA delivery and cancer therapy
... its siRNA loading ...of siRNA. We also demonstrated a new mechanism of siRNA release from PBA-diol crosslinked polycations undergoing disassembly and charge ...accelerated siRNA release was ...
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Development of Non-Covalent Functionalization of Carbon Nanotubes for siRNA Delivery
... condense siRNA electrostatically. Gel shift assay was used to assess the siRNA binding capacity of ...free siRNA migrates along the gel while the migration of siRNA bound to DGI/C would be ...
203
Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
... the delivery system influences intracellular uptake of ...intracellular delivery needs to be determined for each delivery ...intracellular delivery of siRNA compared with nanoparticles ...
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Optimization of siRNA Delivery Method into the Liver by Sequential Injection of Polyglutamic Acid and Cationic Lipoplex
... therapeutic siRNA targets, including cholesterol biosynthesis, fibrosis, hepatitis and hepatocellular ...of siRNA delivery is optimal for liver siRNA therapy, concerted effort from researchers ...
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The pH-Triggered Triblock Nanocarrier Enabled Highly Efficient siRNA Delivery for Cancer Therapy
... of delivery systems in the past ...for siRNA delivery have been developed and it is gradually revealed that enhancing siRNA release from endosomes into cytosol is a very important factor for ...
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Chaperonin–dendrimer conjugates for siRNA delivery
... well siRNA) and the cell viability was measured after 72 ...scrambled siRNA (10 pmol per well) to these cells. THS–PAMAM/KIF11 siRNA reduced the viability of the cells to 79% ± 17% normal- ized to ...
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PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells
... of siRNA by the “pro- ton sponge” effect ...the delivery efficiency for gene silencing, we further co-delivered siRNAs against Cdk1 and Cdk2 that are pivotal cell cycle-regulatory molecules ...siRNAs ...
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SiRNA Delivery with PEGylated Graphene Oxide Nanosheets for Combined Photothermal and Genetherapy for Pancreatic Cancer
... into siRNA delivery systems for biomedical applications [4, 34, ...for siRNA-mediated ...of siRNA in vivo [43], we utilized multifunctional GO as the gene delivery system to target ...
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Research progress on siRNA delivery with nonviral carriers
... Unfortunately, siRNA duplexes are not ideal drug-like ...their delivery, stability, and off-target effects. Delivery systems provide solutions to many of the challenges facing siRNA ...for ...
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RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model
... To investigate the siRNA delivery and silencing effect of pRNA-3WJ RNPs in vivo, U87EGFRvIII-Luc cell-induced brain tumor was prepared into two groups of mice (n=5). At 5, 7 and 9 days post-surgery, 1 mg/kg ...
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Application of quantum dots as vectors in targeted survivin gene siRNA delivery
... are versatile photostable probes that offer an added dimension to improve the power of RNAi as an experimental tool. QDs as luminescent probes for numerous biological and biomedi- cal applications have become an area of ...
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A dual function TAR Decoy serves as an anti HIV siRNA delivery vehicle
... rev siRNA target site corresponding to our previously reported site II was cloned in the siCHECK plasmid ...the siRNA effect, the TAR miRNA was treated with Calf intestinal alkaline phosphatase (CIAP) to ...
9