siRNA screen
Functional genome-wide siRNA screen identifies KIAA0586 as mutated in Joubert syndrome
... whole-genome siRNA experiment and tested on the second replicate and vice versa where a modest AUC of ...centriole siRNA screen, which was a whole-genome siRNA study, was designed to identify ...
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Genome Wide siRNA Screen Identifies Complementary Signaling Pathways Involved in Listeria Infection and Reveals Different Actin Nucleation Mechanisms during Listeria Cell Invasion and Actin Comet Tail Formation
... genome-wide siRNA screen not only confirmed and re- fined previously described signaling pathways involved in this process but also shed new light on the contribution of diverse cellular functions like RNA ...
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siRNA screen of the human signaling proteome identifies the PtdIns(3,4,5)P3 mTOR signaling pathway as a primary regulator of transferrin uptake
... Diced-siRNA screen of the human signaling proteome identifies regulators of transferrin ...the screen data (light blue) is compared to the stochastic noise distribution ...
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A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
... genome-wide siRNA screen with 64,755 siRNAs against 21,566 human genes using an EBOV minigenome system that we had optimized for high-throughput ...this screen, suggesting that the de novo pyrimidine ...
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Pharmacological inhibition of Bcl-xL sensitizes osteosarcoma to doxorubicin
... Our siRNA screen identifies anti-apoptosis genes such as Bcl-xL, Mcl-1 and Bfl-1, but interestingly, it also identified pro-apoptosis genes such as Bak, Bok and ...
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RNA interference screening identifies a novel role for PCTK1/ CDK16 in medulloblastoma with c-Myc amplification
... high-throughput siRNA screen targeting protein and lipid kinases in two clones of medulloblastoma DAOY cells: empty-vector-transfected (V11) or transfected with a c-Myc overexpressing construct ...the ...
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A Genome-Wide Small Interfering RNA Screen Identifies Host Factors Required for Vesicular Stomatitis Virus Infection
... Genome-wide siRNA screen identifies host factors required for VSV ...an siRNA screen specifically opti- mized to monitor a single cycle of viral infection ...this screen, a recombinant ...
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Functional genomics identifies specific vulnerabilities in PTEN deficient breast cancer
... primary siRNA screen in PTEN+ and PTEN- MCF-10A cell ...siGENOME siRNA library from Dharmacon (now ...21,121 siRNA SMARTpools (pools contain- ing 4 different siRNAs targeting each gene) was ...
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A Genome-Wide Small Interfering RNA Screen Identifies Host Factors Required for Vesicular Stomatitis Virus Infection
... secondary screen of 450 host genes isolated from the genome-wide siRNA screen ...genome-wide screen and the demonstration that these genes are required at the step of gene expression support ...
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Screening analysis of ubiquitin ligases reveals G2E3 as a potential target for chemosensitizing cancer cells
... high-content siRNA screen, please see Tab. S1. As expected, our screen identified gene products known to be involved in the DDR and DNA repair, but also in the ...
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Identifying Host Factors Important for Hantavirus Entry
... throughput siRNA screen in a human cell line at BSL-2 ...genetic screen in a human haploid cell line. Follow-up experiments using siRNA knockdown, mutant cell lines, and small molecule ...
152
Identification of Rab18 as an Essential Host Factor for BK Polyomavirus Infection Using a Whole-Genome RNA Interference Screen
... Whole-genome siRNA screening provides an unbiased tool with which to investi- gate some of the remaining ...an siRNA screen assay is finding a readout that is sensitive, cost efficient, and suitable ...
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TRAF6 Affects RAC1 Expression and Apoptosis in SK Hep1 Cells
... TRAF6 siRNA, the expression of RAC1 mRNA significantly decreased (P < ...FADD siRNA and RIP1 siRNA, there was no significant change in RAC1 mRNA (P > ...
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RNAi mediated knock down of Dab and Numb attenuate Aβ levels via γ secretase mediated APP processing
... Western blot analysis was performed as described by Xie et al. [25]. Briefly, 40 μg of total protein of each sample was subjected to SDS-polyacrylamide gel electro- phoresis using 4-20% gradient Tris/glycine gels under ...
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Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
... nanoparticles, and nontargeted EHCO/siRNA nanoparticles modified with arginine-alanine-aspartic acid (RAD)-PEG- Mal, and PEG-Mal, were prepared as control nanoparticles. The mean diameter of all the nanoparticles ...
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Water soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA
... of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery ...as siRNA platform for pulmonary ...of siRNA into sub-300 nm colloidal complexes (at rela- ...
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Development of Non-Covalent Functionalization of Carbon Nanotubes for siRNA Delivery
... the siRNA between FGIS/C and GIS/C in the treated tumors as a whole but the localization of siRNA in the tumor cells which express high level of ...[48], siRNA specifically downregulate a gene and ...
203
Diacylglycerol kinase α controls RCP-dependent integrin trafficking to promote invasive migration.
... and siRNA of DGK- had minimal effects upon 36:1 lipids, demonstrating that PLD activity was ...and siRNA against DGK- were upon the C38 lipids, in particular 38:3, 38:4, and 38:5, pointing to selectivity ...
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Tumor specific delivery and therapy mediate by integrin β6- target immunoliposomes for β6-siRNA in colon carcinoma
... We have previously found that integrinβ6 was able to induce the migration and invasion of colon cancer cells by up-regulation of MMP-3/MMP-9 [23, 24]. In the meantime, the migration and invasion of colon cancer cells ...
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Analysis by siRNA_profile program displays novel thermodynamic characteristics of highly functional siRNA molecules
... the siRNA antisense ...user-friendly siRNA design algorithm with multiple options for minimizing unspe- cific siRNA ...synthetic siRNA molecules, in addition to CpG ...
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