site-directed mutagenesis
Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis
... XynCDBFV (GenBank:KP691331) was synthesized in Generay (Shanghai Generay Biotech Co. Ltd.). The gene of xylanase triple mutant (Xyn-MUT) and three xyla- nase single mutants (N207S, G208S, and A210S) were constructed by ...
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Serratia marcescens B4A chitinase thermostability enhancement by S390I QuikChange site directed mutagenesis
... Serratia marcescens is one of the pioneer bacterium in chitin degradation (Watanabe et al., 1997; Bjørk et al., 2003). S. marcescens B4A chitinase (Babashpour et al., 2012) plays an important role in its plant anti- ...
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The peptide agonist-binding site of the glucagon-like peptide-1 (GLP-1) receptor based on site-directed mutagenesis and knowledge-based modelling
... used site-directed mutagenesis and pharmacological profiling to highlight nine sites as being important for peptide agonist binding and/or ...agonist-binding site itself, were found to be ...
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Cloning, production and site-directed mutagenesis of bovine pancreatic ribonuclease A in Escherichia coli
... Bamase is a ribonuclease from Bacillus amyloliquefaciens, it is composed of a single polypeptide chain with no disulfide bonds. Mossakowska and collaborators (1989) have characterized the enzyme in terms of its basic ...
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Molecular Docking and Site-directed Mutagenesis of a Bacillus thuringiensis Chitinase to Improve Chitinolytic, Synergistic Lepidopteran-larvicidal and Nematicidal Activities
... their mutagenesis leads to remarkably altered activity. Site-directed mutagenesis of these residues consistently resulted in decreased enzyme activity ...
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Site-directed mutagenesis of a conserved hexapeptide in the paramyxovirus hemagglutinin-neuraminidase glycoprotein: effects on antigenic structure and function.
... We have used site-directed mutagenesis and expression of the mutated HN protein of one member of the group, Newcastle disease virus, to explore the role of this highly conserved sequence[r] ...
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Site-Directed Mutagenesis of the Nidovirus Replicative Endoribonuclease NendoU Exerts Pleiotropic Effects on the Arterivirus Life Cycle
... On the basis of the alignment presented in Fig. 1B, both absolutely and less-conserved residues of EAV NendoU were targeted by site-directed mutagenesis and reverse genetics (Ta- ble 1). Each of the ...
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Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6
... 19]. Site- directed mutagenesis based on rational design has been widely used to identify the roles of a specific amino acid ...by site-directed ...
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Effect of site-directed mutagenesis at the GGEEF domain of the biofilm forming GGEEF protein from Vibrio cholerae
... The putative GGD(/E)EF protein VC0395_0300 from the chromosome I of V. cholerae classical strain O395 shows the presence of a GGD(/E)EF domain, as well as a PAS domain signature, as determined from domain prediction ...
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Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9
... restriction site and replaced it with another, providing for easy detection of successful ...seamless site-directed mutagenesis of the yeast genome using CRISPR-Cas9, but did not demonstrate ...
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QuikChange II XL Site-Directed Mutagenesis Kit
... XL site-directed mutagenesis kit is derived from the QuikChange II site-directed mutagenesis ...efficient mutagenesis of large or otherwise difficult-to-mutagenize plasmid ...
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“Natural Site Directed Mutagenesis” Might Exist in Eukaryotic Cells
... “Natural site-directed mutagenesis” in eukaryotic cells is a rare event, and usually results from long-term harmful-agent pressure. Harmful agent, such as CQ, can induce the eukaryotic cells, such a ...
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Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis
... random mutagenesis (a); saturation mutagenesis at residues L141, Y195, and W256 (b); and critical amino acid substitutions by site-directed mutagenesis ...
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Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.
... Plasmids. Restriction enzymes, ligase, DNA polymerase I (Klenow fragment), as well as alkaline phosphatase (calf intestinal) used in molecular cloning were purchased either from Boehringer Mannheim or New England Biolabs ...
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A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
... 'site- directed mutagenesis' an entry clone containing the target DNA fragment is again used as the template for PCR ...attL site at one end and overlapping region with mutated nucleotide(s) ...
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Attenuation of Murray Valley Encephalitis Virus by Site-Directed Mutagenesis of the Hinge and Putative Receptor-Binding Regions of the Envelope Protein
... Mutagenesis of the hinge region at E-277. Four amino acid substitutions at E-277, varying in terms of their size and hy- drophobicity, were selected for this study. At E-277, the hy- drophilic Ser residue was ...
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The phosphorylation targets of p47phox, a subunit of the respiratory burst oxidase Functions of the individual target serines as evaluated by site directed mutagenesis
... To rule out the trivial possibility that the very low oxidase activity observed in cells expressing the mutant p47P/WX resulted from a failure of the mutant to fold into a functionally a[r] ...
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Site-directed mutagenesis of polyomavirus middle-T antigen sequences encoding tyrosine 315 and tyrosine 250.
... Interestingly, it has been demonstrated that tyrosine 250 becomes a prominent in vitro phosphate acceptor site in mutant middle-T antigens lacking other tyrosine residues and also when a[r] ...
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Herpesvirus proteinase: site-directed mutagenesis used to study maturational, release, and inactivation cleavage sites of precursor and to identify a possible catalytic site serine and histidine.
... The R and M cleavage sites are indicated by arrows; three of the five conserved domains CD1 to CD3 are indicated by shaded rectangles; a 15-amino-acid epitope C3 inserted into the amino [r] ...
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Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.
... Because this protocol created a mixture of four different mutations two at each PstI site, the mutagenized PstI linear molecules Simian virus 40 SV40 T-antigen is a multifunctional prote[r] ...
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