Site-Specific Integration Using Phage φ C31 Intergrase
The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase
... exquisite site se- lectivity for productive synapsis, and the architecture of the active recombination ...attP×attB integration reaction by the phage A118 ...
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Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage φC31
... unidirectional site-specific recombination between its attB and attP recognition ...this site-specific integration system also functions efficiently in Drosophila melanogaster in cul- ...
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Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
... cells. Site-specific integration of the viral genome was assessed by PCR amplification of the ITR-AAVS1 junctions by using two sets of nested primers that anneal to the viral ITR and to the ...
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Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185
... We next examined the components of the protein-DNA complexes using anti-His and anti-Flag antibodies which were specific for rTRP-185 and rRep68, respectively. Anti-Flag an- tibody caused a supershift of ...
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HMEJ-mediated efficient site-specific gene integration in chicken cells
... Site-specific integration of foreign genes under en- dogenous promoters or as fusion with an endogenous protein has the advantage of utilizing endogenous gene regulatory ...labeling using a ...
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Combining Comprehensive Analysis of Off Site Lambda Phage Integration with a CRISPR Based Means of Characterizing Downstream Physiology
... at phage integration into secondary ...of phage integration in the presence of the attB site and revealed that lambda targets over 300 unique sites in the host genome for ...that ...
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Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
... system using an episomal Epstein-Barr virus shuttle vector was recently developed to study the adeno-associated virus (AAV) site-specific integration event in chromosome ...after ...
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Site directed biotinylation of filamentous phage structural proteins
... of phage Pro- tein 3 has already been ...peptide specific phages can be produced carrying endogenous biotin moieties with- out the concern of effecting the properties of the affinity ligand and shown to ...
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Isolation of isoform specific binding proteins (Affimers) by phage display using negative selection
... Active Site of the Target Protein The biotinylation reagent (EZ-Link™ NHS-Biotin) reacts with primary amines such as the amino termini of polypeptides or the amine of the side chain of lysine ...
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Rapid establishment of a HEK 293 cell line expressing FVIII BDD using AAV site specific integration plasmids
... Xiaomei Liu, Han Ping and Chun Zhang * Abstract Background: Stable human cell lines have gradually become the preferred system for large scale production of recombinant proteins for clinical applications because of their ...
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Long-Range Targeted Manipulation of the Drosophila Genome by Site-Specific Integration and Recombinational Resolution
... attachment site for the phiC31 integrase previously targeted to the nbs gene, we integrated an 80-kb genomic fragment at its endogenous locus to generate a tandem duplication of the ...a site-specific DNA ...
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Using Phage as A Highly Specific Antibiotic Alternative Against Methicillin Resistance Staphylococcus aureus (MRSA)
... used phage as an antimicrobial agent and have shown them to be effective at destroying target bacteria 33, 40-43 .... Phage are highly specific and are only capable of lysing bacteria and have not ...
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Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... chosen specific site on the cellular ...for site-specific ...triggers site-specific integration at AAVS1 of an ITR-flanked cassette in a ligand- dependent manner, as ...
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Phage-displayed peptide targeting on the Puumala hantavirus neutralization site.
... RESULTS Selection of Puumala virus envelope glycoprotein-specific phage-displayed peptides mimicking neutralizing antibodies. Peptides were selected from a random heptapeptide library flanked by cysteines ...
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Feasibility study for the implementation and the integration of earthquake early warning systems to national scale and for specific site
... magnitude using only three and six stations indicates that: a) location errors larger than 20 kilometers affect mainly offshore sources; b) the combination of a dense seismic network such as the RAN and a robust ...
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Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... purified using a single-step gravity column method described by Auricchio et ...AAVS1 integration by PCR-Southern ...was specific for the right AAV ITR (ITR1, 5⬘-AGGAACCCCT AGTGATGGAG-3⬘), and the ...
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Genome, Integration, and Transduction of a Novel Temperate Phage of Helicobacter pylori
... among phage integrases (PFAM00589): Y354 (corre- sponding to the catalytic site Y342 of phage Int) and the con- served tetrad amino acids (R221, K256, R321, and H344 corre- sponding to R212, K235, ...
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Adeno-Associated Virus Site-Specific Integration and AAVS1 Disruption
... available integration sub- strate ...of integration. Presentation of an integration sub- strate during the time of AAVS1 rearrangement creates the opportunity for a copy choice mechanism to integrate ...
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The phage integrase vector pIPI03 allows RecA independent, site specific labelling of Staphylococcus lugdunensis strains
... A site-specific integrative plasmid for the use in ...attachment site was cloned into the thermosensitive plasmid ...recA-independently, site-specifically and irreversibly into the ...
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C31 Program Proposal Kit
... makes specific decisions about particular ...the C31 website, and it is very important that all producers familiarise themselves with what can and cannot be done within their ...
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