small interfering RNA (siRNA)
A novel dendritic nanocarrier of polyamidoamine-polyethylene glycol-cyclic RGD for “smart” small interfering RNA delivery and in vitro antitumor effects by human ether-à-go-go-related gene silencing in anaplastic thyroid carcinoma cells
... of RNA interference techniques is promising in gene therapeutic approaches, especially for ...of small interfering RNA (siRNA) delivery, a triblock dendritic nanocarrier, ...
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Sorting nexin-4 regulates β-amyloid production by modulating β-site-activating cleavage enzyme-1
... Scrambled small interfering RNA; siRNA: Small interfering RNA; siSNX4: Small interfering RNA mixture; SNX: Sorting nexin; SNX4: Sorting nexin-4; ...
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Original Article TRAF4 promotes the growth and invasion of colon cancer through the Wnt/β-catenin pathway
... and small interfering RNA (siRNA)-mediated gene knockdown of TRAF4 significantly inhibited cell proliferation, invasion and tumorigenesis, both in vitro and in vivo, but induced apoptosis in ...
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In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi
... Using small size (small interfering) RNA (siRNA), the effect was observed on the parasite viability, the mf release, the adverse effect on the embryogenesis in the female worm, and ...
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Original Article Neuroepithelial transforming gene 1 controls proliferation and migration of non-small cell lung cancer A549 cells via regulating RhoA
... Non small-cell lung cancer (NSCLC), the main cause of cancer deaths worldwide, lacks effective treat- ...by small interfering RNA, and then Real-time quantitative PCRs (qRT-PCRs) and western ...
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... of small interfering RNA (siRNA)-mediated silencing of NAMPT and LYST on cell proliferation and apoptosis were evaluated in RPMI 8226 myeloma ...in siRNA-treated cells was estimated by ...
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Incorporation of osteogenic and angiogenic small interfering RNAs into chitosan sponge for bone tissue engineering
... two small interfering RNAs (siRNAs), ie, small interfering RNA targets casein kinase 2 interaction protein 1 (siCkip-1) and small interfering RNA targets soluble ...
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Original Article LOXL4 is downregulated in hepatocellular carcinoma with a favorable prognosis
... LOXL4 small interfering RNA (siRNA) interference to investigate the biological function of LOXL4, LOXL4 immunohistochemical staining was performed using a tissue microarray containing 298 HCC ...
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Original Article miR-10b represses the proliferation and invasion of prostate cancer by targeting LRH1
... Abstract: Liver receptor homolog 1 (LRH1) plays an important role in the onset and progression of many cancer types. However, the role of LRH1 in prostate cancer has not been well investigated. In this study, the ...
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Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis
... with small interfering RNA (siRNA) for NFκB1 or a scrambled sequence control siRNA, after which the cells were further incubated in culture medium with stem cell factor (10 ng/ml), ...
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Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases
... The rotavirus nonstructural protein NSP1 acts as an antagonist of the host antiviral response by inducing degradation of key proteins required to activate interferon (IFN) production. Protein degradation induced by NSP1 ...
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<p>Latest Advances of Long Non-Coding RNA SNHG5 in Human Cancers</p>
... non-coding RNA; SNHG5, small nucleolar RNA host gene 5; ncRNA, non-coding RNA; miRNA, microRNA; siRNA, small interfering RNA; tRNA, transfer RNA; rRNA, ...
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Transient and Stable Knockdown of the Integrase Cofactor LEDGF/p75 Reveals Its Role in the Replication Cycle of Human Immunodeficiency Virus
... Transient small interfering RNA-mediated knock- down of LEDGF/p75 in HeLaP4 cells resulted in a three- to fivefold inhibition of HIV-1 (strain ...hairpin RNA (shRNA) targeting this ...
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Determining the Involvement and Therapeutic Implications of Host Cellular Factors in Hepatitis C Virus Cell-to-Cell Spread
... intracellular RNA was purified using an ABI PRISM 6100 Nucleic Acid PrepStation (Applied Biosys- tems), as per the manufacturer’s ...Purified RNA was used for cDNA synthesis using TaqMan reverse ...
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Therapeutic application of RNAi: is mRNA targeting finally ready for prime time?
... speed, RNA interference (RNAi) has advanced from its basic discovery in lower organisms to becoming a powerful genetic tool and perhaps our single most promising biotherapeutic for a wide array of dis- ...
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Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 3 Inhibits Gamma Interferon and Major Histocompatibility Complex Class II Expression
... by RNA interference (RNAi) and genome-wide mRNA expression pro- files were determined by microarray ...An siRNA without ho- mology to any human or viral gene (siN) served as a ...
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Expression of the RNA binding protein RBM3 is associated with a favourable prognosis and cisplatin sensitivity in epithelial ovarian cancer
... Total RNA isolation (RNeasy, QIAgen, Hilden, Ger- many), cDNA synthesis (Reverse Transcriptase kit, Applied Biosystems, Warrington, UK) and quantitative real-time PCR (qRT-PCR) analysis with SYBR Green PCR master ...
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<p>The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface</p>
... Purpose: Zirconia is one of the most promising implant materials due to its favorable physical, mechanical and biological properties. However, until now, we know little about the mechanism of osseointegration on ...
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Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery
... for siRNA delivery to DCs to suppress immune responses ...of siRNA-loaded lipid ...of siRNA delivery system (Figure 2A, ...groups. siRNA molecules had been designed to target CD40, CD80 and ...
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Antitumor therapeutic application of self-assembled RNAi-AuNP nanoconstructs: Combination of VEGF-RNAi and photothermal ablation
... therapeutic siRNA loading capacity and efficiently complexed with a cationic agent of branched polyethylenimine (PEI) to form condensed and compact nanoparticles, denoted as PEI/RNAi-AuNP, for effective ...
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