Soft agar
Organ culture of seminiferous tubules using a modified soft agar culture system
... colonization of neonate mouse and spermatogonial stem cells in a SACS, along with leukemia inhibitory factor (LIF) and glia cell line-derived neurotrophic factor (GDNF). Supplementation of melatonin and these factors in ...
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The effects of melatonin on colonization of neonate spermatogonial mouse stem cells in a three-dimensional soft agar culture system
... The soft agar culture system (SACS) is a qualitative, three- dimensional cell culture structure that was first used for clonal expansion of bone marrow cells and exploration of factors that are associated ...
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Systematic analysis of secreted proteins reveals synergism between IL6 and other proteins in soft agar growth of MCF10A cells
... After 24 hours, the infected MCF10A cells were tryp- sinized, counted, and seeded in puromycin (1 μ g/ml) containing soft agar (2 × 10 6 cell/15 cm dish). The dishes were maintained for four weeks, and 15 ...
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Encapsulation of Staphylococcus aureus isolates from mastitic milk: relationship between capsular polysaccharide types 5 and 8 and colony morphology in serum soft agar, clumping factor, teichoic acid, and protein A
... Encapsulation of Staphylococcus aureus Isolates from Mastitic Milk: Relationship between Capsular Polysaccharide Types 5 and 8 and Colony Morphology in Serum-Soft Agar, Clumping Factor, [r] ...
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Demonstration of the capsular antigens of bovine group B streptococci by the serum soft agar method
... Serum-soft agar analyses of the diffuse-type growth of the conventional encapsulated group B streptococcal serotypes Ia 090, Ib H36B, Ic A909, II 18RS21, and III D136C and the compact-ty[r] ...
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FAK is a Critical Regulator of Neuroblastoma Liver Metastasis
... used soft agar colony assay to assess for anchorage-independent cell growth, which is a well-established indicator of tumorigenicity of cancer cells in vitro ...
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Summary: Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives
... In a soft agar colony formation assay, IO and 5I-IO showed potent effects; 5SA-IO was excluded from the assay. With increasing concentrations, compound-treated cells[r] ...
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TAZ and YAP are frequently activated oncoproteins in sarcomas
... TAZ (WWTR1) and YAP are transcriptional coactivators and oncoproteins inhibited by the Hippo pathway. Herein we evaluate 159 sarcomas representing the most prevalent sarcoma types by immunohistochemistry for expression ...
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MiR-384 inhibits the proliferation of colorectal cancer by targeting AKT3
... MTT, soft agar and colony formation assay were performed with the stable cells of SW480/miR-384, SW480/Vector, HCT116/miR-384 and HCT116/Vector ...of soft agar and colony formation assay of ...
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A benign cultured colon adenoma bears three genetically altered colon cancer oncogenes, but progresses to tumorigenicity and transforming growth factor beta independence without inactivating the p53 tumor suppressor gene
... We describe the spontaneous progression of a colon adenoma cell line to tumorigenicity and growth factor independence. This system allows direct comparison of biologic stages of malignant progression with alterations of ...
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Amplification-driven BCL6-suppressed cytostasis is mediated by transrepression of FOXO3 and post-translational modifications of FOXO3 in urinary bladder urothelial carcinoma
... growth, (D) upregulated CCND1, CDK4, CCNE1, CDK2, E2F1 and TFDP1 protein levels compared to the control (pHaloTag transfectant), however, notably downregulated TP53, pTP53(S15), pTP53(S20), CDKN1A, CDKN1B, CDKN1C, ...
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Adaptor protein Ruk/CIN85 modulates resistance to doxorubicin of murine 4T1 breast cancer cells
... – Soft agar colonies formation ability of 4T1 cells with different levels of Ruk/ cIN85 expression levels in the presence or absence of doxorubicin (Wt – wild-type 4t1 cells; Mock – 4t1 cells transfected ...
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Key roles of necroptotic factors in promoting tumor growth
... Thus far our experiments suggest a clear role for all three necroptotic genes in sustaining the tumorigenicity of malignant cells. We next carried out experiments to examine whether the chemical compound necrosulfonamide ...
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TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways
... using soft agar formation assays, whose results showed that TLE3 overexpression also repressed the proliferation of SW480 and Ls174t cells in soft agar, as both the number and size of colonies ...
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Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration
... Polarity proteins play an important role in regulating the protein complexes that form tight, adherence and gap junctions. The apical localization of Par3 initiates cell- cell junctions and it localizes to tight ...
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L 428 nodular sclerosing Hodgkin's cell secretes a unique transforming growth factor beta active at physiologic pH
... Biological activity of A Hodgkin's TGF was compared with TGF-f I from - platelets and is shown to have similar biological activities: A AKR-2B colony formation in soft agar, B inhibition[r] ...
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Iridin Attenuates the Migration and Attachment Independent Growth of Lung Adenocarcinoma Cells
... Abstract: Lung adenocarcinoma is the leading cause of lung cancer related deaths worldwide. The low survival rates associated with this cancer are being attributed to metastasis and recurrence which contribute to the ...
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Murine gamma interferon inhibits v-mos-induced fibroblast transformation via down regulation of retroviral gene expression.
... Thus, although the soft agar growth of 3T3mos4 No LT R mos DISCUSSION We show here that murine recombinant IFN-y exerts a strong, but reversible, inhibition of retroviral gene expression[r] ...
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Alterations of the three short open reading frames in the Rous sarcoma virus leader RNA modulate viral replication and gene expression.
... Reverse transcriptase assays on samples of extracellular media assay 1 provided a quantitative measure of the number of released viral particles; focus formation assays in soft agar assa[r] ...
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Preclinical validation of 3-phosphoinositide-dependent protein kinase 1 inhibition in pancreatic cancer
... assessed by soft agar assays (Fig. 3 a-c, Additional file 2: Figure S3a). PDK1 inhibition also reduced anchorage- independent growth in CFPAC-1 (Additional file 2: Figure S1c,d), PANC-1 (Additional file 2: ...
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