Solutions for acute hippocampal brain slices
Chronic nicotine treatment differentially modifies acute nicotine and alcohol actions on GABA A and glutamate receptors in hippocampal brain slices
... and a4b2* nAChRs mediate the enhancement of GABAergic transmission in the hippocampal CA1 neuronal circuitry. As ethanol inhibits the a7* nAChRs, it neutralizes the nicotine potentiation of glutamatergic ...
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A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
... prepared slices, was a prominent margin due to-as suggested here- errors in ...the slices pre- sented with noticeable frames of PI-detectable ...thus slices with such a prominent margin distorted the ...
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Dense arrays of micro-needles for recording and electrical stimulation of neural activity in acute brain slices
... in acute slices of brain tissue. Approach. Acute slices, arguably the closest in-vitro model of the brain, have a damaged surface ...in acute cortical slices with a ...
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Targeting neurotransmitter receptors with nanoparticles in vivo allows single molecule tracking in acute brain slices
... organotypic slices, which provide the great advantage of an easy and direct access to superficial cells 15 ...thick acute brain slices has thus been a major challenge that has bogged down our ...
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Original Article Dexmedetomidine inhibits epileptiform activity in rat hippocampal slices
... of hippocampal brain slic- es prepared from 3- to 6-week-old Sprague- Dawley rats (80-150 g, either ...The brain of each rat was then rapidly removed from the skull and placed in a cold (3-4°C), ...
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β-Adrenoceptor activation depresses brain inflammation and is neuroprotective in lipopolysaccharide-induced sensitization to oxygen-glucose deprivation in organotypic hippocampal slices
... and slices were assorted into four groups with one slice from each individual mouse per experimental group and six slices per ...group. Slices were incubated with propi- dium iodide (PI, ...
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Verbal Episodic Memory Alterations and Hippocampal Atrophy in Acute Mild Traumatic Brain Injury
... the acute phase, volume normalization in the subacute phase, and subsequent volume reduction in the chronic phase following an ...the brain lead to oxidative stress causing cellular dysfunction and death ...
Non-associative hippocampal long-term depression (LTD) and associative neocortal long-term potentation of synaptic transmission in rat brain slices
... the slices with acsf containing 25 mM Mg^"^ and 2 mM ...of slices to 25 mM Mg^"^ 2 Ca^^ resulted in block of both orthodromic and antidromic responses at all depths within the slice in 2 to 5 ...
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Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi Electrode Array System and Laser Confocal Calcium Imaging
... Electric signals in the brain can be measured by the conventional electrophy- siological methods. By intracellular or extracellular recording, direct measure- ment from a single neuron or neural area is possible ...
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Administering Copper Blocks CA1 Neuron Hyper Excitability in Rat Hippocampal Slices
... the acute copper administration and its subsequent discontinuity can be related to the over-ex- pression of glutamatergic receptors as a consequence of temporal blocking of the glutamatergic ...in ...
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Functional MRI during hippocampal deep brain stimulation in the healthy rat brain
... maps. Slices progress from most anterior at the left to most posterior at the ...The hippocampal struc- tures (HC), lateral thalamic structures (lThal), medial thalamic structures (mThal), septal nuclei ...
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Plastination and Staining of Brain Slices Using Two Different Dehydration Methods
... fixative solutions, mainly those which contain formaldehyde, are considered toxic and prolonged exposure to them in an indoor environment may be a risk factor for people who handle the specimens or inhale the ...
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beta Hydroxybutyrate fuels synaptic function during development Histological and physiological evidence in rat hippocampal slices
... pocampal slices were not observed after the suckling period, suggesting that in the mature brain, the failure to use D - b HB as an energy source is not simply explained by a decrease in circulating levels ...
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A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
... the brain macrophages, ...the brain has been studied extensively [7-11], most progress on the understanding of the role of microglia in inflammation has come from cell culture and slice culture ...
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Research Article Properties of Taurine Release in Glucose-Free Media in Hippocampal Slices from Developing and Adult Mice
... developing brain taurine release is much larger in magnitude than in the adult brain, which could be a reason for the well- known greater tolerance of developing nervous tissue to lack of ...
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Assessment of glutamatergic synaptic transmission and plasticity in brain slices: relevance to bioelectronic approaches
... and brain biochemistry for the imple- mentation of bioelectronic approaches in the ...vivo hippocampal slices and electrophysiological ...The hippocampal slice is an ideal preparation because ...
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Temporal Lobe Epilepsy With Hippocampal Sclerosis in Acute Lymphoblastic Leukemia
... with hippocampal sclerosis (MTLE-HS) is characterized by intractable epilepsy with complex partial seizures (CPSs) of temporal origin and temporal sclero- sis and represents the fi nal common pathway of several ...
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Measurement of network activity in acute slices of the healthy and parkinsonian striatum on microelectrode arrays
... in acute slices In order to understand neural activity in neurological diseases, it is possible to prepare neural tissue from lab animals, which can then be kept alive or even grown into a new network by ...
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Interactive Effects of Exercise, Sex Hormones, and Transient Congenital Hypothyroidism on Long-Term Potentiation in Hippocampal Slices of Rat Offspring
... Transverse slices (450 μm) were prepared with a Vi- broslice (Campden Instruments, Silbey, ...The slices were stored in a brain slice keeper oxygenated with 95% O 2 /5% CO 2 at room temperature for ...
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Investigating the synchronization of hippocampal neural network in response to acute nicotine exposure
... Using borosilicate glass electrodes (borosilicate micropi- pettes, WPI, Sarasota, FL) with a pulled tip of about 1 μm, extracellular field potential recordings were made on the stratum pyramidale of the CA1 cell layer. ...
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