Sp1 binding sites
Variable role of the long terminal repeat Sp1-binding sites in human immunodeficiency virus replication in T lymphocytes.
... 1414 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The long terminal repeat LTR of the human immunodeficiency virus HIV contains three binding sites for the transcrip[r] ...
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Examination of the roles of transcription factor Sp1-binding sites and an octamer motif in trans induction of the herpes simplex virus thymidine kinase gene.
... Several observations made attractive the hypothesis that increases in the activity or availability of Spl mediate trans induction: i experiments in which tk promoter sequences were fused[r] ...
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Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells.
... Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest The 21-bp repeat region of simian virus 40 SV40 activates viral transcription and DNA replication and contains binding si[r] ...
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SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
... putative Sp1 binding sites in Zbtb7Acore promoter sequence: primer forward, 5'- GGGAAACTGAGGCT- GATGG-3', reverse, 5'- TCAGGTGTCCCACTCCCAAC-3' for-1003 bp to -925 bp amplification, and primers for- ...
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Autostimulation of the Epstein-Barr Virus BRLF1 Promoter Is Mediated through Consensus Sp1 and Sp3 Binding Sites
... Two Sp1 sites within Rp are crucial for activation by ...contains binding sites for several other transcription factors ...two Sp1 binding sites at ⫺45 and ⫺279, two ...
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Identification and Functional Characterization of Cis-Regulatory Elements Controlling Expression of the Porcine ADRB2 Gene
... Sp1 binding sites (GC-boxes), potential recognition sites for transcription factors binding GC-rich se- quences such as early growth response gene 1 (Egr1), Myc-associated zinc finger ...
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Differential Regulation of Hepatitis B Virus Gene Expression by the Sp1 Transcription Factor
... on Sp1, and hence abolishing Sp1 binding to this enhancer ele- ment results in the suppression of all of the HBV promoter ...the Sp1 binding sites in the ENII enhancer and the ...
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HTLV 1 HBZ cooperates with JunD to enhance transcription of the human telomerase reverse transcriptase gene (hTERT)
... affinity binding sites for the yeast protein Gal4 upstream of a minimal TATA box (5XGal4-Luc) by 11-fold (Fig 6, compare lanes 3 and 2), when it was coexpressed with a Gal4-Sp1B chimeric pro- tein ...
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Functional Differences between the Long Terminal Repeat Transcriptional Promoters of Human Immunodeficiency Virus Type 1 Subtypes A through G
... complete Sp1 region through prolonged cul- turing of an attenuated HIV-1 subtype B virus, yielding a stronger LTR promoter with six Sp1 sites and a fitter virus ...of Sp1 binding ...
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Regulation of LRRK2 promoter activity and gene expression by Sp1
... putative Sp1 binding sites were functional, EMSA was conducted to examine the binding between Sp1 and these promoter regions in vitro ...3c). Sp1 expression plasmid ...
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Temozolomide analog PMX 465 downregulates MGMT expression in HCT116 colorectal carcinoma cells
... multiple Sp1 binding sites, and transcription factor Sp1 binding could promote MGMT expression(Bocangel et ...detect Sp1 expression in HCT116 cells after treatment (48 h) with ...
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“Calling Cards” for DNA-Binding Proteins in Mammalian Cells
... transcription-factor binding events throughout the development of an organism would facilitate mapping of transcriptional networks that control cell-fate ...factor SP1 fused to the piggyBac transposase ...
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MDM2 promoter SNP285 and SNP309; phylogeny and impact on cancer risk
... enhanced Sp1 transcription factor binding and elevated MDM2 ...Assessing Sp1 binding to the MDM2 promoter using surface plasmon resonance technology, we found SNP309G to enhance Sp1 ...
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CaMELS : In silicoprediction of calmodulin binding proteins and their binding sites
... CaM binding sites within a protein can help biologists in elucidating underlying biological processes at the molecular ...dependent binding and the large sequence diversity of its targets, ...
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Two Sp1/Sp3 Binding Sites in the Major Immediate-Early Proximal Enhancer of Human Cytomegalovirus Have a Significant Role in Viral Replication
... cloning sites upstream of the chloramphenicol acetyltransferase (CAT) ...with Sp1( ⫺ 55) (from position ⫺ 58 to ⫹ 53), the MIE promoter with Sp1( ⫺ 55) and Sp1(⫺75) (from ⫺77 to ⫹53), or the ...
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Structure prediction, molecular dynamics simulation and docking studies of d-specific dehalogenase from rhizobium sp. Rc1
... It was observed that the minimum constricted segment diameter of the predicted DehD active site channel was at least 0.4 Å. The electrostatic surface into this channel was highly basic compared to the rest of the ...
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Multiple high affinity binding sites for
... (1.2±0.2 mg protein ml −1 ). Following incubation at 25 °C for 45 min, the assay mixture was rapidly filtered under vacuum through Whatman GF/B glass fibre filters using a Brandel cell harvester, and rinsed four times in ...
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An overview of pharmacodynamic modelling, ligand binding approach and its application in clinical practice
... Antagonist: A drug that binds to a receptor but does not eli- cit a response is referred to as an antagonist. Importantly, the antagonist must block the action of the agonist at the receptor site. Antagonist can shift ...
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The potent enhancer activity of the polycythemic strain of spleen focus-forming virus in hematopoietic cells is governed by a binding site for Sp1 in the upstream control region and by a unique enhancer core motif, creating an exclusive target for PEBP/CBF.
... enhancer binding protein/core binding factor (PEBP/CBF) and excludes access of CAAT/enhancer binding ...the Sp1 motif in the UCR stimulates transactivation through the Ets-PEBP ...
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Methylation of Human Papillomavirus Type 16 CpG Sites at E2 Binding Site 1 (E2BS1), E2BS2, and the Sp1 Binding Site in Cervical Cancer Samples as Determined by High Resolution Melting Analysis–PCR
... and Sp1-binding site (Sp1BS) has frequently been associated with cer- vical cancer (28, 29, 35–38), and a gradual increase in methylation during lesion progression toward cancer has been reported in some ...
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